HSP90 beta Antibody - BSA Free

Western Blot: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: HSP90 beta Antibody [NBP1-77563] - Analysis of HSP901b in: 1. HeLa, 2. Ntera 2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. PC12 and 8. COS7.

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP1-77563] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry: HSP90 beta Antibody [NBP1-77563] - Staining of HSP901b in mouse prostate using DAB with hematoxylin counterstain.

Simple Western: HSP90 beta Antibody [NBP1-77563] - Simple Western lane view shows a specific band for Hsp90B in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HSP90 beta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSP90 beta Antibody - BSA Free (NBP1-77563) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-HSP90 beta Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal portion of the human Hsp90B protein (between residues 650-724) [UniProt P08238]
• Localization: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSP90AB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Simple Western 1:1000
  • Western Blot 1 ug/ml
• Application Notes: This HSP90-1B antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections, and Western blot where a band is seen ~83 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 93 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Immunogen has 86% identity to cow.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSP90 beta Antibody - BSA Free

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), also known as HSPC2, HSPCB and HSP90B, is a chaperone that plays an integral part in signal transduction, protein folding and degradation, cell cycle regulation, and protein maturation. Specifically, HSP90AB1 is a highly conserved chaperone which interacts with other cochaperones to assist in either the folding or refolding of proteins. HSP90AB1 is a constitutive form of the two major HSP90 proteins and is found primarily in the cytosol along with the inducible form, HSP90AA1. HSP90B is a target molecule for treatment of Helicobacter pylori (H. pylori)-induced gastric injury due to an interaction of HSP90B1 with Rac1 ultimately leading to increased production in reactive oxygen species (ROS) in gastric epithelial cells (PMID: 20451655). HSP90B in conjunction with HSP70 also plays a role in HIF-1alpha stability via ubiquitin and O(2)-independent regulation (PMID: 19802386). HSP90 antibodies are useful tools for studying cancer or other diseases brought on by cellular stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP90 beta Antibody (NBP1-77563)(1)

Publication using NBP1-77563

• He X, Smith SE, Chen S et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche Cell reports 2021-09-07 [PMID: 34496236]

Bioinformatics

• Gene Symbol: HSP90AB1
• Entrez:
  • 767874(Bovine)
  • 3326(Human)
  • 15516(Mouse)
  • 301252(Rat)
• Uniprot:
  • P08238()