HSP40/DNAJB1 Antibody (OTI1F9)

Images

 
Western Blot: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp40 monoclonal antibody (HepG2: human; HeLa: human; SVT2: ...read more
Immunocytochemistry/ Immunofluorescence: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp40.
Immunohistochemistry-Paraffin: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) Staining of paraffin-embedded Human prostate tissue using anti-Hsp40 mouse monoclonal antibody.
Flow Cytometry: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ...read more
Western Blot: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp40 (Right lane) cDNA for 48 hrs and lysed. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.6 mg/ml

Order Details

HSP40/DNAJB1 Antibody (OTI1F9) Summary

Immunogen
Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DNAJB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP40/DNAJB1 Antibody (OTI1F9)

  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • DnaJ (Hsp40) homolog, subfmaily B, member 1
  • dnaJ homolog subfamily B member 1
  • DnaJ protein homolog 1
  • DNAJ1
  • DNAJB1
  • Hdj1
  • hDj-1
  • Heat shock 40 kDa protein 1
  • heat shock 40kD protein 1
  • Heat shock protein 40
  • HSP40
  • HSPF1
  • HSPF1Hdj1
  • Human DnaJ protein 1
  • radial spoke 16 homolog B
  • RSPH16B
  • Sis1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DNAJB1
Entrez