Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA, 2 Plate

Images

 
Lysates prepared from heat-shocked, human Jurkat cells were incubated in wells coated with HSP-70 Duoset IC Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample ...read more
Jurkat cells were heat-shocked at 44ºC for 30 mins and allowed to recover for 4 hrs at 37 ºC. Following cell lysis, HSP-70 was quantified with the DuoSet IC. The same lysates were also immunoblotted (inset) with Rb ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

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Catalog# & Conjugate Size Price

Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA, 2 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
HSPA1A

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DYC1663-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA, 2 Plate

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

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Publications for HSP70/HSPA1A (DYC1663-2)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Ovine.


Filter By Application
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Filter By Species
Human
(12)
Mouse
(6)
Rat
(1)
Ovine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using DYC1663-2 Applications Species
P Innamarato, J Morse, A Mackay, S Asby, M Beatty, J Blauvelt, S Kidd, JE Mullinax, AA Sarnaik, S Pilon-Thom Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors BMC Cancer, 2021;21(1):756. 2021 [PMID: 34187428] (Mouse) Mouse
RJ Playford, N Choudhry, P Kelly, T Marchbank Effects of Bovine Colostrum with or without Egg on In Vitro Bacterial-Induced Intestinal Damage with Relevance for SIBO and Infectious Diarrhea Nutrients, 2021;13(3):. 2021 [PMID: 33809940] (Human) Human
T Ratschker, L Egenberger, M Alev, L Zschiesche, J Band, E Schreiber, B Frey, A Derer, C Alexiou, C Janko Mitoxantrone-Loaded Nanoparticles for Magnetically Controlled Tumor Therapy-Induction of Tumor Cell Death, Release of Danger Signals and Activation of Immune Cells Pharmaceutics, 2020;12(10):. 2020 [PMID: 32992645] (Human) Human
M Hader, DP Savcigil, A Rosin, P Ponfick, S Gekle, M Wadepohl, S Bekeschus, R Fietkau, B Frey, E Schlücker, US Gaipl Differences of the Immune Phenotype of Breast Cancer Cells after Ex Vivo Hyperthermia by Warm-Water or Microwave Radiation in a Closed-Loop System Alone or in Combination with Radiotherapy Cancers (Basel), 2020;12(5):. 2020 [PMID: 32349284] (Human) Human
B Theivanthi, KS Evans, NC DeVito, MP Plebanek, M Sturdivant, LP Wachsmuth, AK Salama, Y Kang, D Hsu, JM Balko, DB Johnson, M Starr, AB Nixon, A Holtzhause, BA Hanks A tumor-intrinsic PD-L1-NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32017708] (Mouse) Mouse
A Kühnel, D Schilling, SE Combs, B Haller, M Schwab, G Multhoff Radiosensitization of HSF-1 Knockdown Lung Cancer Cells by Low Concentrations of Hsp90 Inhibitor NVP-AUY922 Cells, 2019;8(10):. 2019 [PMID: 31569342] (Human) Human
T Haider, E Simader, O Glück, HJ Ankersmit, T Heinz, S Hajdu, LL Negrin Systemic release of heat-shock protein 27 and 70 following severe trauma Sci Rep, 2019;9(1):9595. 2019 [PMID: 31270381] (Human) Human
J Nam, S Son, LJ Ochyl, R Kuai, A Schwendema, JJ Moon Chemo-photothermal therapy combination elicits anti-tumor immunity against advanced metastatic cancer Nat Commun, 2018;9(1):1074. 2018 [PMID: 29540781] (Mouse) Mouse
A Dyer, Y Di, H Calderon, S Illingwort, G Kueberuwa, A Tedcastle, P Jakeman, SL Chia, A Brown, MA Silva, D Barlow, J Beadle, T Hermiston, DJ Ferguson, B Champion, KD Fisher, LW Seymour Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory Mediators Mol Ther Oncolytics, 2017;4(0):18-30. 2017 [PMID: 28345021] (Human) Human
Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 27994152] (Rat) Rat
Show All 22 Publications.

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Bioinformatics

Gene Symbol HSPA1A