HDAC5 [p Ser498] Antibody - BSA Free

Western Blot: HDAC5 [p Ser498] Antibody [NB100-81953] - Analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5 (Phospho-Ser498) antibody

Immunohistochemistry-Paraffin: HDAC5 [p Ser498] Antibody [NB100-81953] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81953 incubated with blocking peptide(right).

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HDAC5 [p Ser498] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HDAC5 [p Ser498] Antibody - BSA Free (NB100-81953) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 498 (T-Q-SP-S-P).
• Modification: p Ser498
• Specificity: Detects endogenous levels of HDAC5 only when phosphorylated at serine 498.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC5 [p Ser498] Antibody - BSA Free

• Antigen NY-CO-9
• EC 3.5.1.98
• FLJ90614
• HD5
• Histone Deacetylase 5
• KIAA0600
• NY-CO-9

Background

HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC5
• Entrez:
  • 10014(Human)
  • 15184(Mouse)
  • 84580(Rat)
• Uniprot:
  • Q9UQL6(Human)