Western Blot: GFAP Antibody [NB100-53809] - Human cerebellum (A), Mouse (B) and Rat (C) brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/ml. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GFAP Antibody [NB100-53809] - (0.01ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB ...read more
Immunohistochemistry-Paraffin: GFAP Antibody [NB100-53809] - (2ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, ...read more
ELISA: GFAP Antibody [NB100-53809] - (5ug/ml) as the reporter with the capture rabbit antibody (5ug/ml). This data is from a previous batch, not on sale.
Peptide with sequence C-DGEVIKESKQEHKD corresponding to C-Terminus according to NP_002046.1.
GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Immunogen affinity purified
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Western blot: Approx 50 kDa band observed in rat brain lysates (calculated MW of 49.9 kDa according to NP_058705.2). Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on human and mouse brain for this particular batch.
Predicted cross-reactivity based on sequence identity: Human, Mouse and Canine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GFAP Antibody
glial fibrillary acidic protein
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post.
GFAP: Roles in Alzheimer's and Schizophrenia Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a... Read full blog post.
Vimentin in Wound Healing Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p... Read full blog post.
"Whats the Hap" with GFAP? Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi... Read full blog post.
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