GFAP Antibody (5C10)

Images

 
Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution ...read more
Simple Western: GFAP Antibody (5C10) [NBP1-05197] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - The immunofluorescence of TLR7 recognized by Alexa 488, green (NBP2-24906). GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged ...read more
Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse mAb to GFAP, NBP1-05197, dilution 1:1,000, in green, costained with rabbit pAb to neurofilament NF-L, ...read more
Western Blot: GFAP Antibody (5C10) [NBP1-05197] - Analysis of whole tissue lysates using mouse mAb to GFAP, NBP1-05197, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC
Clone
5C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GFAP Antibody (5C10) Summary

Immunogen
This GFAP Antibody (5C10) was developed against a preparation of purified pig spinal cord GFAP
Localization
Cytoplasm. Note: Associated with intermediate filaments.
Marker
Astrocyte Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry Free-Floating 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Simple Western 1:3000
  • Western Blot 1:5000
Application Notes
This GFAP (5C10) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections, and Western blot. In WB, a band can be seen at approx. 50 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-05197 in the following applications:

Publications
Read Publications using
NBP1-05197 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol
Preservative
5mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFAP Antibody (5C10)

  • ALXDRD
  • FLJ45472
  • GFAP astrocytes
  • GFAP
  • glial fibrillary acidic protein

Background

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFAP Antibody (NBP1-05197)(25)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: B/N, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC/IF, WB.


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B/N
(3)
ICC/IF
(9)
IF/IHC
(5)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
IHC/IF
(1)
WB
(2)
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Human
(1)
Mouse
(11)
Rat
(4)
Porcine
(1)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NBP1-05197 Applications Species
Duan Q, Zhang Q, Nie K et al. Myo1d Promotes Alpha-Synuclein Transfer from Brain Microvascular Endothelial Cells to Pericytes through Tunneling Nanotubes iScience 2023-07-01 [PMID: 37575183] (ICC/IF, Mouse)

Details:
Dilutions: 1:100
ICC/IF Mouse
Rueda-Gensini L, Serna JA, Rubio D et al. Three-dimensional neuroimmune co-culture system for modeling Parkinson's Disease microenvironments in vitro Biofabrication 2023-06-27 [PMID: 37369196] (ICC/IF, Human) ICC/IF Human
Jahnke L, Zandi S, Elhelbawi A et al. Characterization of Macroglia Response during Tissue Repair in a Laser-Induced Model of Retinal Degeneration International Journal of Molecular Sciences 2023-05-24 [PMID: 37298126] (IHC-P, Mouse)

Details:
1:200 IHC-P dilution
IHC-P Mouse
Tseng KY, Stratoulias V, Hu WF et al. Augmenting hematoma-scavenging capacity of innate immune cells by CDNF reduces brain injury and promotes functional recovery after intracerebral hemorrhage Cell death & disease 2023-02-15 [PMID: 36792604] (IHC-Fr, Mouse) IHC-Fr Mouse
Wong JK, Lin J, Kung NJ et al. Cerebrospinal fluid immunoglobulins in primary progressive multiple sclerosis are pathogenic Brain : a journal of neurology 2023-02-03 [PMID: 36732292] (IHC, Mouse) IHC Mouse
Griflyuk AV, Postnikova TY, Zaitsev AV. Prolonged Febrile Seizures Impair Synaptic Plasticity and Alter Developmental Pattern of Glial Fibrillary Acidic Protein (GFAP)-Immunoreactive Astrocytes in the Hippocampus of Young Rats International Journal of Molecular Sciences 2022-10-13 [PMID: 36293077] (B/N, ICC/IF) B/N, ICC/IF
Becker-Krail DD, Ketchesin KD, Burns JN et al. Astrocyte Molecular Clock Function in the Nucleus Accumbens Is Important for Reward-Related Behavior Biological Psychiatry 2022-07-01 [PMID: 35461698] (B/N) B/N
Postnikova TY, Diespirov GP, Amakhin DV et al. Impairments of Long-Term Synaptic Plasticity in the Hippocampus of Young Rats during the Latent Phase of the Lithium-Pilocarpine Model of Temporal Lobe Epilepsy International Journal of Molecular Sciences 2021-12-12 [PMID: 34948152] (ICC/IF, B/N, IHC) ICC/IF, B/N, IHC
Fayaz MA, Rosa GDS, Honaramooz A. Neonatal Porcine Germ Cells Dedifferentiate and Display Osteogenic and Pluripotency Properties Cells 2021-10-20 [PMID: 34831039] (ICC/IF) ICC/IF
Scaricamazza S, Salvatori I, Amadio S et al. Repurposing of Trimetazidine for amyotrophic lateral sclerosis: A study in SOD1(G93A) mice British Journal of Pharmacology 2022-04-01 [PMID: 34783031]
Show All 25 Publications.

Review for GFAP Antibody (NBP1-05197) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-05197:
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IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence GFAP NBP1-05197
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3
reviewed by:
Verified Customer
IF Mouse 05/11/2016
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Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
Lot5313

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFAP Antibody (NBP1-05197). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

Secondary Antibodies

 

Isotype Controls

Additional GFAP Products

Research Areas for GFAP Antibody (NBP1-05197)

Find related products by research area.

Blogs on GFAP. Showing 1-10 of 14 blog posts - Show all blog posts.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Astrocytes: Diversity in type and function
By Michalina Hanzel, PhDAstrocytes are a type of macroglia found in the central nervous system that regulate a vast array of essential brain functions, ranging from synaptogenesis, ion homeostasis and maintenance of t...  Read full blog post.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by ...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Verified Customer
05/11/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GFAP
Entrez