Biological Strategies: Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry: G9a/EHMT2 Antibody [NB100-40825] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Whole cell lysate (15 and 50 ug) from HeLa cells. Antibody used for WB at 1 ug/ml.
The immunogen recognized by this antibody maps to a region between residue 1160 and the C-terminus (residue 1210) of human G9A (Euchromatic Histone-Lysine N-methyltransferase 2) using the numbering given in entry NP_006700.2 (GeneID 10919).
Immunogen affinity purified
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While trace amounts of G9A/EHMT2 can be immunoprecipitated by this antibody, the efficiency is very low. Use in Rat reported in customer review. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
histone-lysine N-methyltransferase, H3 lysine-9 specific 3
HLA-B associated transcript 8
Lysine N-methyltransferase 1C
G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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