G9a/EHMT2 Antibody

Images

 
Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunohistochemistry: G9a/EHMT2 Antibody [NB100-40825] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Whole cell lysate (15 and 50 ug) from HeLa cells. Antibody used for WB at 1 ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC-P, IP, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

G9a/EHMT2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1160 and the C-terminus (residue 1210) of human G9A (Euchromatic Histone-Lysine N-methyltransferase 2) using the numbering given in entry NP_006700.2 (GeneID 10919).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EHMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
While trace amounts of G9A/EHMT2 can be immunoprecipitated by this antibody, the efficiency is very low. Use in Rat reported in customer review. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
G9a/EHMT2 Lysate (NBL1-10164)
Reviewed Applications
Read 1 Review rated 3
using
NB100-40825 in the following applications:

Publications
Read Publications using NB100-40825.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for G9a/EHMT2 Antibody

  • ankyrin repeat-containing protein
  • BAT8
  • C6orf30
  • chromosome 6 open reading frame 30
  • DKFZp686H08213
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT2
  • euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8
  • FLJ35547
  • G9A histone methyltransferase
  • G9a
  • G9AEm:AF134726.3
  • H3-K9-HMTase 3
  • Histone H3-K9 methyltransferase 3
  • histone-lysine N-methyltransferase, H3 lysine-9 specific 3
  • HLA-B associated transcript 8
  • KMT1CNG36/G9a
  • Lysine N-methyltransferase 1C
  • NG36
  • Protein G9a

Background

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for G9a/EHMT2 Antibody (NB100-40825)(2)

Review for G9a/EHMT2 Antibody (NB100-40825) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-40825:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/26/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysate, Sample Amount: 50 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBS-T

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesPositive Control: 293T Cell Lysate

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
01/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol EHMT2
Entrez
Uniprot