EZH2/KMT6 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ...read more
Measurement of EZH2 protein under conditions of SIRT1 knockdown. SIRT1 knockdown in Caco-2 cells was achieved using two different siRNAs and protein was analysed by Western blotting using anti-EZH2 antibody (a) or ...read more
EZH2 elevates at S phase in hDPCs and interacts with PCNA. a Schematic chart illustrating the serum deprivation approach used for G0/G1 phase synchronization of hDPCs. b Western blot shows the expression of EZH2 in ...read more
EZH2 elevates at S phase in hDPCs and interacts with PCNA. a Schematic chart illustrating the serum deprivation approach used for G0/G1 phase synchronization of hDPCs. b Western blot shows the expression of EZH2 in ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

EZH2/KMT6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EZH2
Gly512-Ile645
Accession # Q15910
Specificity
Detects human and mouse EZH2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EZH2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4767 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EZH2/KMT6 Antibody [Unconjugated]

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

EZH2 (Enhancer of zeste homolog 2; also ENX-1 and Lys N-methyltransferase 6) is an 80 kDa member of the EZ family of chromatin-dependent gene regulators. It is a nuclear protein that represses gene transcription through histone methylation. Human EZH2 is 746 amino acids (aa) in length. It contains an NLS (aa 490‑495), a Cys-rich region, and a methyltransferase SET (Suppressor/Enhancer/Trithorax) domain (aa 606‑729). There are four potential splice variants. One shows a premature truncation after Cys286, a second shows a 6 aa substitution for aa 329‑746, a third shows a deletion of aa 83‑121, and the fourth exhibits a 5 aa insertion after His297. Over aa 512‑645, human, mouse and canine EZH2 are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EZH2/KMT6 Antibody (AF4767)(2)

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Video Protocols

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Bioinformatics

Gene Symbol EZH2
Uniprot