NRSF Antibody (CL0381) - Azide and BSA Free

Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.

Staining of human cerebral cortex shows no nuclear positivity in neuronal cells as expected.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Staining of U-2 OS cells using the Anti-NRSF monoclonal antibody) .

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL0381
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NRSF Antibody (CL0381) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NRSF Antibody (CL0381) - Azide and BSA Free (NBP2-42048) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q13127, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: REST
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NRSF Antibody (CL0381) - Azide and BSA Free

• Neural-restrictive silencer factor
• NRSFneuron restrictive silencer factor
• RE1-silencing transcription factor
• X2 box repressor
• XBRrepressor binding to the X2 box

Background

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: REST