G9a/EHMT2 Overexpression Lysate

Images

 
Western Blot: EHMT2 Overexpression Lysate (Adult Normal) [NBL1-10164] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EHMT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP

Order Details

G9a/EHMT2 Overexpression Lysate Summary

Description
Transient overexpression lysate of euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI The lysate was created in HEK293T cells, using Plasmid ID RC219200 and based on accession number NM_025256. The protein contains a C-MYC/DDK Tag.
Gene
EHMT2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 23884959).

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
128.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBL1-10164 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23884959)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for G9a/EHMT2 Overexpression Lysate

  • ankyrin repeat-containing protein
  • BAT8
  • C6orf30
  • chromosome 6 open reading frame 30
  • DKFZp686H08213
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT2
  • euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8
  • FLJ35547
  • G9A histone methyltransferase
  • G9a
  • G9AEm:AF134726.3
  • H3-K9-HMTase 3
  • Histone H3-K9 methyltransferase 3
  • histone-lysine N-methyltransferase, H3 lysine-9 specific 3
  • HLA-B associated transcript 8
  • KMT1CNG36/G9a
  • Lysine N-methyltransferase 1C
  • NG36
  • Protein G9a

Background

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq]. Transcript Variant: This variant (NG36/G9a-SPI) lacks 102 bases (exon 10) in the coding region, as compared to variant NG36/G9a, but maintains the same reading frame. The encoded isoform b lacks 34 internal amino acids. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for G9a/EHMT2 Lysate (NBL1-10164)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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NBL1-10164

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Bioinformatics

Gene Symbol EHMT2