GLP/EHMT1 Antibody (B0422) [Unconjugated]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP, ELISA
Clone
B0422
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Suitable Sample Type
23005

Order Details

GLP/EHMT1 Antibody (B0422) [Unconjugated] Summary

Immunogen
Recombinant mouse GLP/EHMT1
aa 134-234
Specificity
This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EHMT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Direct ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Reviewed Applications
Read 5 Reviews rated 4.2
using
PP-B0422-00 in the following applications:

Publications
Read Publications using
PP-B0422-00 in the following applications:

Packaging, Storage & Formulations

Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer
A liquid formulation in physiologic saline with 0.1% NaN3.
Preservative
0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLP/EHMT1 Antibody (B0422) [Unconjugated]

  • DEL9q34
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT1
  • euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
  • Eu-HMTase1
  • Eu-HMTase1DEL9q34
  • EUHMTASE1FP13812
  • FLJ12879
  • G9a-like protein 1
  • GLP
  • GLP1
  • H3 lysine-9 specific 5
  • KIAA1876euchromatic histone methyltransferase 1
  • KMT1D
  • KMT1DDKFZp667M072
  • Lysine N-methyltransferase 1D

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLP/EHMT1 Antibody (PP-B0422-00)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, Immunoprecipitation, Western Blot.


Filter By Application
IHC
(3)
Immunoprecipitation
(3)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using PP-B0422-00 Applications Species
T Dahlet, M Truss, U Frede, H Al Adhami, AF Bardet, M Dumas, J Vallet, J Chicher, P Hammann, S Kottnik, P Hansen, U Luz, G Alvarez, G Auclair, J Hecht, PN Robinson, C Hagemeier, M Weber E2F6 initiates stable epigenetic silencing of germline genes during embryonic development Nature Communications, 2021;12(1):3582. 2021 [PMID: 34117224] (Western Blot, Mouse) Western Blot Mouse
J Yuan, SY Chang, SG Yin, ZY Liu, X Cheng, XJ Liu, Q Jiang, G Gao, DY Lin, XL Kang, SW Ye, Z Chen, JA Yin, P Hao, L Jiang, SQ Cai Two conserved epigenetic regulators prevent healthy ageing Nature, 2020;0(0):. 2020 [PMID: 32103178] (Immunoprecipitation, Human) Immunoprecipitation Human
JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst Elife, 2018;7(0):. 2018 [PMID: 29745895] (IHC, Mouse) IHC Mouse
Shengjiang Tu Co-repressor CBFA2T2 regulates pluripotency and germline development Nature, 2016;534(7607):387-90. 2016 [PMID: 27281218] (IHC, Mouse) IHC Mouse
Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, Kaur J, Kuhlmann T, Zhang W, Slesinger P, Casaccia P Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci, 2015;35(1):352-65. 2015 [PMID: 25568127] (Immunoprecipitation, Rat) Immunoprecipitation Rat
Loh S, Ng W, Yeo K, Lim Y, Ea C Inhibition of euchromatic histone methyltransferase 1 and 2 sensitizes chronic myeloid leukemia cells to interferon treatment. PLoS ONE, 2014;9(7):e103915. 2014 [PMID: 25079219] (Western Blot, Human) Western Blot Human
Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev, 2014;28(4):317-27. 2014 [PMID: 24532712] (Western Blot, Human) Western Blot Human
Ea C, Hao S, Yeo K, Baltimore D EHMT1 protein binds to nuclear factor-kappaB p50 and represses gene expression. J Biol Chem, 2012;287(37):31207-17. 2012 [PMID: 22801426] (Immunoprecipitation, Human) Immunoprecipitation Human
Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, Battisti V, Fritsch L, Lin WM, Hollmann TJ, Ferre F, Bourque C, Burke CJ, Turner L, Uong A, Johnson LA, Beroukhim R, Mermel CH, Loda M, Ait-Si-Ali S, Garraway LA, Young RA, Zon LI The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset. Nature, 2011;471(7339):513-7. 2011 [PMID: 21430779] (Western Blot, Human) Western Blot Human
Schaefer A, Sampath SC, Intrator A Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron, 2009;64(5):678-91. 2009 [PMID: 20005824] (IHC, Mouse) IHC Mouse
Show All 11 Publications.

Reviews for GLP/EHMT1 Antibody (PP-B0422-00) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using PP-B0422-00:
Filter by Applications
ChIP
(1)
WB
(2)
ICC
(1)
IP
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Arunava Roy
ChIP Human 06/26/2018
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedB cells
SpeciesHuman
LotA-2
Western Blot GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Anandita Ghosh
WB Human 06/22/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
LotA-2
Immunocytochemistry GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Arunava Roy
ICC Human 07/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman Dermal Microvascular Endothelial cells - Paraformaldehyde fixed cells
SpeciesHuman
LotA-2
Immunoprecipitation GLP/EHMT1 PP-B0422-00
Enlarge
5
reviewed by:
Arunava Roy
IP Human 04/21/2017
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
LotA-2
Western Blot GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Arunava Roy
WB Human 04/21/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
LotA-2

Product General Protocols

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Video Protocols

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FAQs for GLP/EHMT1 Antibody (PP-B0422-00) (0)

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Secondary Antibodies

 

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Arunava Roy
06/26/2018
Application: ChIP
Species: Human

Anandita Ghosh
06/22/2018
Application: WB
Species: Human

Arunava Roy
07/11/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol EHMT1