GLP/EHMT1 Antibody (B0422) [Unconjugated]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP, ELISA
Clone
B0422
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Suitable Sample Type
23005

Order Details

GLP/EHMT1 Antibody (B0422) [Unconjugated] Summary

Immunogen
Recombinant mouse GLP/EHMT1
aa 134-234
Specificity
This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EHMT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Direct ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Reviewed Applications
Read 5 Reviews rated 4.2
using
PP-B0422-00 in the following applications:

Publications
Read Publications using
PP-B0422-00 in the following applications:

Packaging, Storage & Formulations

Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer
A liquid formulation in physiologic saline with 0.1% NaN3.
Preservative
0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLP/EHMT1 Antibody (B0422) [Unconjugated]

  • DEL9q34
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT1
  • euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
  • Eu-HMTase1
  • Eu-HMTase1DEL9q34
  • EUHMTASE1FP13812
  • FLJ12879
  • G9a-like protein 1
  • GLP
  • GLP1
  • H3 lysine-9 specific 5
  • KIAA1876euchromatic histone methyltransferase 1
  • KMT1D
  • KMT1DDKFZp667M072
  • Lysine N-methyltransferase 1D

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLP/EHMT1 Antibody (PP-B0422-00)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, Immunoprecipitation, Western Blot.


Filter By Application
IHC
(3)
Immunoprecipitation
(3)
Western Blot
(7)
All Applications
Filter By Species
Human
(5)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using PP-B0422-00 Applications Species
Able, AA;Richard, AJ;Stephens, JM; TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1) Biology 2023-04-30 [PMID: 37237488] (Western Blot, Mouse) Western Blot Mouse
Jyothishmathi Swaminathan, Shinji Maegawa, Shavali Shaik, Ajay Sharma, Javiera Bravo-Alegria, Lei Guo et al. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis Frontiers in Oncology 2022-05-06 [PMID: 35600406]
T Dahlet, M Truss, U Frede, H Al Adhami, AF Bardet, M Dumas, J Vallet, J Chicher, P Hammann, S Kottnik, P Hansen, U Luz, G Alvarez, G Auclair, J Hecht, PN Robinson, C Hagemeier, M Weber E2F6 initiates stable epigenetic silencing of germline genes during embryonic development Nature Communications, 2021-06-11;12(1):3582. 2021-06-11 [PMID: 34117224] (Western Blot, Mouse) Western Blot Mouse
J Yuan, SY Chang, SG Yin, ZY Liu, X Cheng, XJ Liu, Q Jiang, G Gao, DY Lin, XL Kang, SW Ye, Z Chen, JA Yin, P Hao, L Jiang, SQ Cai Two conserved epigenetic regulators prevent healthy ageing Nature, 2020-02-26;0(0):. 2020-02-26 [PMID: 32103178] (Immunoprecipitation, Human) Immunoprecipitation Human
JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst Elife, 2018-05-10;7(0):. 2018-05-10 [PMID: 29745895] (IHC, Mouse) IHC Mouse
Shengjiang Tu Co-repressor CBFA2T2 regulates pluripotency and germline development Nature, 2016-06-08;534(7607):387-90. 2016-06-08 [PMID: 27281218] (IHC, Mouse) IHC Mouse
Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al. Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆ Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2016-07-01 [PMID: 26952936]
Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee et al. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development eLife 2015-11-09 [PMID: 26551560]
Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, Kaur J, Kuhlmann T, Zhang W, Slesinger P, Casaccia P Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci, 2015-01-07;35(1):352-65. 2015-01-07 [PMID: 25568127] (Immunoprecipitation, Western Blot, Rat) Immunoprecipitation, Western Blot Rat
Monica Di Giacomo, Stefano Comazzetto, Srihari C Sampath, Srinath C Sampath, Dónal O’Carroll G9a co-suppresses LINE1 elements in spermatogonia Epigenetics & Chromatin 2014-12-01 [PMID: 25276231]
Show All 17 Publications.

Reviews for GLP/EHMT1 Antibody (PP-B0422-00) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using PP-B0422-00:
Filter by Applications
ChIP
(1)
WB
(2)
ICC
(1)
IP
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation GLP/EHMT1 PP-B0422-00
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4
reviewed by:
Verified Customer
ChIP Human 06/26/2018
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedB cells
SpeciesHuman
LotA-2
Western Blot GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Verified Customer
WB Human 06/22/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
LotA-2
Immunocytochemistry GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Verified Customer
ICC Human 07/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman Dermal Microvascular Endothelial cells - Paraformaldehyde fixed cells
SpeciesHuman
LotA-2
Immunoprecipitation GLP/EHMT1 PP-B0422-00
Enlarge
5
reviewed by:
Verified Customer
IP Human 04/21/2017
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
LotA-2
Western Blot GLP/EHMT1 PP-B0422-00
Enlarge
4
reviewed by:
Verified Customer
WB Human 04/21/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
LotA-2

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GLP/EHMT1 Antibody (PP-B0422-00) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Verified Customer
06/26/2018
Application: ChIP
Species: Human

Verified Customer
06/22/2018
Application: WB
Species: Human

Verified Customer
07/11/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol EHMT1