GLP/EHMT1 Antibody (B0422) [Unconjugated]

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ELISA
• Clone: B0422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23005

GLP/EHMT1 Antibody (B0422) [Unconjugated] Summary

• Immunogen: Recombinant mouse GLP/EHMT1
  aa 134-234
• Specificity: This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EHMT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Direct ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLP/EHMT1 Antibody (B0422) [Unconjugated]

• DEL9q34
• EC 2.1.1.-
• EC 2.1.1.43
• EHMT1
• euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
• Eu-HMTase1
• Eu-HMTase1DEL9q34
• EUHMTASE1FP13812
• FLJ12879
• G9a-like protein 1
• GLP
• GLP1
• H3 lysine-9 specific 5
• KIAA1876euchromatic histone methyltransferase 1
• KMT1D
• KMT1DDKFZp667M072
• Lysine N-methyltransferase 1D

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLP/EHMT1 Antibody (PP-B0422-00)(19)

Publications using PP-B0422-00

• Able, AA;Richard, AJ;Stephens, JM; TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1) Biology 2023-04-30 [PMID: 37237488] (Western Blot, Mouse)
• Jyothishmathi Swaminathan, Shinji Maegawa, Shavali Shaik, Ajay Sharma, Javiera Bravo-Alegria, Lei Guo et al. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis Frontiers in Oncology 2022-05-06 [PMID: 35600406]
• T Dahlet, M Truss, U Frede, H Al Adhami, AF Bardet, M Dumas, J Vallet, J Chicher, P Hammann, S Kottnik, P Hansen, U Luz, G Alvarez, G Auclair, J Hecht, PN Robinson, C Hagemeier, M Weber E2F6 initiates stable epigenetic silencing of germline genes during embryonic development Nature Communications, 2021-06-11;12(1):3582. 2021-06-11 [PMID: 34117224] (Western Blot, Mouse)
• J Yuan, SY Chang, SG Yin, ZY Liu, X Cheng, XJ Liu, Q Jiang, G Gao, DY Lin, XL Kang, SW Ye, Z Chen, JA Yin, P Hao, L Jiang, SQ Cai Two conserved epigenetic regulators prevent healthy ageing Nature, 2020-02-26;0(0):. 2020-02-26 [PMID: 32103178] (Immunoprecipitation, Human)
• Roy A, Ghosh A, Kumar B, Chandran B IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP Elife 11/4/2019 [PMID: 31682228]
• JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst Elife, 2018-05-10;7(0):. 2018-05-10 [PMID: 29745895] (IHC, Mouse)
• Shengjiang Tu Co-repressor CBFA2T2 regulates pluripotency and germline development Nature, 2016-06-08;534(7607):387-90. 2016-06-08 [PMID: 27281218] (IHC, Mouse)
• Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al. Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆ Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2016-07-01 [PMID: 26952936]
• Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee et al. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development eLife 2015-11-09 [PMID: 26551560]
• Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, Kaur J, Kuhlmann T, Zhang W, Slesinger P, Casaccia P Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci, 2015-01-07;35(1):352-65. 2015-01-07 [PMID: 25568127] (Immunoprecipitation, Western Blot, Rat)
• Monica Di Giacomo, Stefano Comazzetto, Srihari C Sampath, Srinath C Sampath, Dónal O’Carroll G9a co-suppresses LINE1 elements in spermatogonia Epigenetics & Chromatin 2014-12-01 [PMID: 25276231]
• Loh S, Ng W, Yeo K, Lim Y, Ea C Inhibition of euchromatic histone methyltransferase 1 and 2 sensitizes chronic myeloid leukemia cells to interferon treatment. PLoS ONE, 2014-07-31;9(7):e103915. 2014-07-31 [PMID: 25079219] (Western Blot, Human)
• Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev, 2014-02-15;28(4):317-27. 2014-02-15 [PMID: 24532712] (Western Blot, Human)
• Irina A Maksakova, Peter J Thompson, Preeti Goyal, Steven JM Jones, Prim B Singh, Mohammad M Karimi et al. Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific retrotransposon MERVL in mouse ES cells Epigenetics & Chromatin 2013-12-01 [PMID: 23735015]
• Mund A, Schubert T, Staege H et al. SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response Nucleic Acids Res 10/2/2012 [PMID: 23034801]
• Ea C, Hao S, Yeo K, Baltimore D EHMT1 protein binds to nuclear factor-kappaB p50 and represses gene expression. J Biol Chem, 2012-07-16;287(37):31207-17. 2012-07-16 [PMID: 22801426] (Immunoprecipitation, Human)
• Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, Battisti V, Fritsch L, Lin WM, Hollmann TJ, Ferre F, Bourque C, Burke CJ, Turner L, Uong A, Johnson LA, Beroukhim R, Mermel CH, Loda M, Ait-Si-Ali S, Garraway LA, Young RA, Zon LI The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset. Nature, 2011-03-24;471(7339):513-7. 2011-03-24 [PMID: 21430779] (Western Blot, Human)
• Schaefer A, Sampath SC, Intrator A Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron, 2009-12-10;64(5):678-91. 2009-12-10 [PMID: 20005824] (IHC, Mouse)
• Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity. EMBO J, 2008-09-25;27(20):2691-701. 2008-09-25 [PMID: 18818693] (Western Blot, Mouse)

Reviews for GLP/EHMT1 Antibody (PP-B0422-00) (5)

Average Rating: 4.2

(Based on 5 reviews)

We have 5 reviews tested in 1 species: Human.

reviewed by: Verified Customer ChIP Human 06/26/2018

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: B cells
• Species: Human
• Lot: A-2

reviewed by: Verified Customer WB Human 06/22/2018

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: A-2

reviewed by: Verified Customer ICC Human 07/11/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Dermal Microvascular Endothelial cells - Paraformaldehyde fixed cells
• Species: Human
• Lot: A-2

reviewed by: Verified Customer IP Human 04/21/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human
• Lot: A-2

reviewed by: Verified Customer WB Human 04/21/2017

Summary

• Application: Western Blot
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human
• Lot: A-2

Bioinformatics

• Gene Symbol: EHMT1