HP1 alpha Antibody (2H4E9) - BSA Free

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using HP1 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), and A431 (5) cell lysate.

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of Hela cells using HP1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon tissues using CBX5 mouse mAb with DAB staining.

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon cancer tissues using CBX5 mouse mAb with DAB staining.

ELISA: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, CyTOF-ready
• Clone: 2H4E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HP1 alpha Antibody (2H4E9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human HP1 alpha (AA: 1-191) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBX5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 22.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HP1 alpha Antibody (2H4E9) - BSA Free

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HP1 alpha Antibody (NBP2-52420)(2)

Publications using NBP2-52420

• Lazarchuk P, Nguyen MM, Curca CM et Al. Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells Aging (Albany NY) 2024-10-31 [PMID: 39422615]
• Mata-Garrido J, Xiang Y, Chang-Marchand Y et al. The Heterochromatin protein 1 is a regulator in RNA splicing precision deficient in ulcerative colitis Nature communications 2022-11-18 [PMID: 36400769] (WB, IHC-P, Human, Mouse)

Bioinformatics

• Gene Symbol: CBX5