GOLGA6 Antibody

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Immunohistochemistry-Paraffin: GOLGA6 Antibody [NB100-2436] - Paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GOLGA6 Antibody Summary

Immunogen
Peptide with sequence C-DLPKEKADGTEQ corresponding to internal region according to NP_061122.4, NP_001033729.1.
Specificity
GeneID 55889 may be a pseudogene, while GeneID 342096 is thought to encode a functional protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GOLGA6A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human liver lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9 kDa band according to NP_001033729.1. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebral cortex shows localized cytoplasm staining consistent with Golgi.
Publications
Read Publication using NB100-2436.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GOLGA6 Antibody

  • FLJ75859
  • GLPGolgin-like protein
  • GOLGA6Golgin linked to PML
  • golgi autoantigen, golgin subfamily a, 6
  • golgi autoantigen, golgin subfamily a, 6A
  • golgi autoantigen, golgin subfamily a, member 6
  • golgin A6 family, member A
  • Golgin subfamily A member 6A

Background

GOLGA6 is a member of the golgin family of proteins, whose members localise to the Golgi appartus. This gene is found in a large, low copy repeat sequence or duplicon that is found in multiple copies, that are greater than 90% similar, on chromosome 15. Duplicons are associated with deletions, inversions and other chromosome rearrangements that underlie genomic disease. The protein encoded by this gene is thought to be a functional golgin protein while the majority of the related copies of this gene are thought to be transcribed pseudogenes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GOLGA6 Antibody (NB100-2436)(1)

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Bioinformatics

Gene Symbol GOLGA6A
Entrez
Uniprot