Western Blot: SETDB1 Antibody [NBP2-20322] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.
Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.
Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The ...read more
SETDB1 antibody validated for ICC from a verified customer review ChIP assay- Assay dependent. Use in ICC/IF and Proximity Ligation Assay reported in scientific literature (PMID:31682228).
143 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 4 Reviews rated 3.8 using NBP2-20322 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Immunogen affinity purified
Alternate Names for SETDB1 Antibody
ERG-associated protein with SET domain
Histone H3-K9 methyltransferase 4
histone-lysine N-methyltransferase SETDB1
histone-lysine N-methyltransferase, H3lysine-9 specific 4
KMT1EERG-associated protein with a SET domain, ESET
Lysine N-methyltransferase 1E
SET domain bifurcated 1
SET domain, bifurcated 1
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for SETDB1 Antibody (NBP2-20322)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 5 applications: ChIP, ICC/IF, IP, PLA, WB.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.