SETDB1 Antibody

Images

 
Western Blot: SETDB1 Antibody [NBP2-20322] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.
Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.
Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IP, PLA, ChIP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SETDB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (93%), Rat (90%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETDB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:100-1:500
  • Proximity Ligation Assay
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
Application Notes
SETDB1 antibody validated for ICC from a verified customer review ChIP assay- Assay dependent. Use in ICC/IF and Proximity Ligation Assay reported in scientific literature (PMID:31682228).
Theoretical MW
143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 3.8
using
NBP2-20322 in the following applications:

Publications
Read Publication using
NBP2-20322 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • PLA
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for SETDB1 Antibody

  • ERG-associated protein with SET domain
  • ESETEC 2.1.1.43
  • H3-K9-HMTase 4
  • Histone H3-K9 methyltransferase 4
  • histone-lysine N-methyltransferase SETDB1
  • histone-lysine N-methyltransferase, H3lysine-9 specific 4
  • KG1T
  • KIAA0067H3-K9-HMTase4
  • KMT1EERG-associated protein with a SET domain, ESET
  • Lysine N-methyltransferase 1E
  • SET domain bifurcated 1
  • SET domain, bifurcated 1

Background

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SETDB1 Antibody (NBP2-20322)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ChIP, ICC/IF, IP, PLA, WB.


Filter By Application
ChIP
(1)
ICC/IF
(1)
IP
(1)
PLA
(1)
WB
(1)
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Filter By Species
Human
(1)
All Species

Reviews for SETDB1 Antibody (NBP2-20322) (4) 3.84

Average Rating: 3.8
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NBP2-20322:
Filter by Applications
WB
(2)
ICC
(1)
IP
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot SETDB1 NBP2-20322
Enlarge
4
reviewed by:
Anonymous
WB Human 06/18/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
Lot40471
Immunocytochemistry SETDB1 NBP2-20322
Enlarge
3
reviewed by:
Anonymous
ICC Human 07/12/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman Dermal Microvascular Endothelial Cells - Paraformaldehyde Fixed
SpeciesHuman
Lot40471
Immunoprecipitation SETDB1 NBP2-20322
Enlarge
4
reviewed by:
Anonymous
IP Human 04/20/2017
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
Lot40471
Western Blot SETDB1 NBP2-20322
Enlarge
4
reviewed by:
Anonymous
WB Human 04/19/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman Dermal Microvascular Endothelial whole cell lysate
SpeciesHuman
Lot40471

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SETDB1 Antibody (NBP2-20322) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Learn the difference between western blot and simple western

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Calculators

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Recent Reviews

3.8
5
0
4
3
3
1
2
0
1
0

Anonymous
06/18/2018
Application: WB
Species: Human

Anonymous
07/12/2017
Application: ICC
Species: Human

Anonymous
04/20/2017
Application: IP
Species: Human

Bioinformatics

Gene Symbol SETDB1
Uniprot