Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The ...read more
Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.
Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The ...read more
SETDB1 antibody validated for ICC from a verified customer review. ChIP assay-, IHC-P- Assay dependent. Use in ICC/IF and Proximity Ligation Assay reported in scientific literature (PMID:31682228).
143 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 4 Reviews rated 3.8 using NBP2-20322 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris-Glycine (pH7), 20% Glycerol
Immunogen affinity purified
Alternate Names for SETDB1 Antibody
ERG-associated protein with SET domain
Histone H3-K9 methyltransferase 4
histone-lysine N-methyltransferase SETDB1
histone-lysine N-methyltransferase, H3lysine-9 specific 4
KMT1EERG-associated protein with a SET domain, ESET
Lysine N-methyltransferase 1E
SET domain bifurcated 1
SET domain, bifurcated 1
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for SETDB1 Antibody (NBP2-20322)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 5 applications: ChIP, ICC/IF, IP, PLA, WB.
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