SETDB1 Antibody

Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.

Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IHC-P, IP, PLA, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SETDB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (90%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETDB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Proximity Ligation Assay
  • Western Blot 1:500-1:3000
• Application Notes: SETDB1 antibody validated for ICC from a verified customer review. ChIP assay-, IHC-P- Assay dependent. Use in ICC/IF and Proximity Ligation Assay reported in scientific literature (PMID:31682228).
• Theoretical MW: 143 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for SETDB1 Antibody

• ERG-associated protein with SET domain
• ESETEC 2.1.1.43
• H3-K9-HMTase 4
• Histone H3-K9 methyltransferase 4
• histone-lysine N-methyltransferase SETDB1
• histone-lysine N-methyltransferase, H3lysine-9 specific 4
• KG1T
• KIAA0067H3-K9-HMTase4
• KMT1EERG-associated protein with a SET domain, ESET
• Lysine N-methyltransferase 1E
• SET domain bifurcated 1
• SET domain, bifurcated 1

Background

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SETDB1 Antibody (NBP2-20322)(1)

Publication using NBP2-20322

• Roy A, Ghosh A, Kumar B, Chandran B IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP Elife 2019-11-04 [PMID: 31682228] (ChIP, ICC/IF, IP, PLA, WB, Human)

Reviews for SETDB1 Antibody (NBP2-20322) (4)

Average Rating: 3.8

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Human.

reviewed by: Anandita Ghosh WB Human 06/18/2018

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: 40471

reviewed by: Arunava Roy ICC Human 07/12/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Dermal Microvascular Endothelial Cells - Paraformaldehyde Fixed
• Species: Human
• Lot: 40471

reviewed by: Arunava Roy IP Human 04/20/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human
• Lot: 40471

reviewed by: Arunava Roy WB Human 04/19/2017

Summary

• Application: Western Blot
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human
• Lot: 40471

Bioinformatics

• Gene Symbol: SETDB1
• Uniprot:
  • Q15047(Human)