FXR/NR1H4 Antibody (A9033A) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ELISA
Clone
A9033A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Suitable Sample Type
23005

Order Details

FXR/NR1H4 Antibody (A9033A) [Unconjugated] Summary

Immunogen
Recombinant human FXR/NR1H4
aa 2-126
Specificity
This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR1H4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
  • Direct ELISA
Reviewed Applications
Read 2 Reviews rated 5
using
PP-A9033A-00 in the following applications:

Publications
Read Publications using
PP-A9033A-00 in the following applications:

Packaging, Storage & Formulations

Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer
A liquid formulation in physiologic saline with 0.1% NaN3
Preservative
0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FXR/NR1H4 Antibody (A9033A) [Unconjugated]

  • BAR
  • bile acid receptor
  • farnesoid X receptor
  • Farnesoid X-activated receptor
  • Farnesol receptor HRR-1
  • FXR
  • FXRBAR
  • HRR-1
  • HRR1MGC163445
  • NR1H4
  • Nuclear receptor subfamily 1 group H member 4
  • nuclear receptor subfamily 1, group H, member 4
  • Retinoid X receptor-interacting protein 14
  • RIP14
  • RIP14RXR-interacting protein 14

Background

Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IP, ELISA

Publications for FXR/NR1H4 Antibody (PP-A9033A-00)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, Immunoprecipitation, Western Blot.


Filter By Application
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using PP-A9033A-00 Applications Species
L Absil, F Journé, D Larsimont, JJ Body, L Tafforeau, D Nonclercq Farnesoid X receptor as marker of osteotropism of breast cancers through its role in the osteomimetism of tumor cells BMC Cancer, 2020;20(1):640. 2020 [PMID: 32650752] (IHC, Human) IHC Human
HM Hassan, M Isovic, B Kolendowsk, N Bauer-Mais, O Onabote, M Cecchini, A Haig, S Maleki Var, TM Underhill, J Torchia Loss of Thymine DNA Glycosylase Causes Dysregulation of Bile Acid Homeostasis and Hepatocellular Carcinoma Cell Rep, 2020;31(1):107475. 2020 [PMID: 32268085] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Immunomodulatory glycan LNFPIII alleviates hepatosteatosis and insulin resistance through direct and indirect control of metabolic pathways. Nat Med, 2012;18(11):1665-72. 2012 [PMID: 23104131] (Western Blot, Mouse) Western Blot Mouse
Deuschle U, Schuler J, Schulz A, Schluter T, Kinzel O, Abel U, Kremoser C FXR Controls the Tumor Suppressor NDRG2 and FXR Agonists Reduce Liver Tumor Growth and Metastasis in an Orthotopic Mouse Xenograft Model. PLoS ONE, 2012;7(10):e43044. 2012 [PMID: 23056173] (Western Blot, Human) Western Blot Human
Prade E, Tobiasch M, Hitkova I, Schaffer I, Lian F, Xing X, Tanzer M, Rauser S, Walch A, Feith M, Post S, Rocken C, Schmid RM, Ebert MP, Burgermeister E Bile acids down-regulate caveolin-1 in esophageal epithelial cells through sterol responsive element-binding protein. Mol. Endocrinol., 2012;26(5):819-32. 2012 [PMID: 22474125] (Western Blot, Human) Western Blot Human
Lian F, Xing X, Yuan G, Schafer C, Rauser S, Walch A, Rocken C, Ebeling M, Wright MB, Schmid RM, Ebert MP, Burgermeister E Farnesoid X receptor protects human and murine gastric epithelial cells against inflammation-induced damage. Biochem. J., 2011;438(2):315-23. 2011 [PMID: 21619550] (Western Blot, Human) Western Blot Human
Lee JY, Lee KT, Lee JK, Lee KH, Jang KT, Heo JS, Choi SH, Kim Y, Rhee JC Farnesoid X receptor, overexpressed in pancreatic cancer with lymph node metastasis promotes cell migration and invasion. Br. J. Cancer, 2011;104(6):1027-37. 2011 [PMID: 21364590] (Western Blot, Human) Western Blot Human

Reviews for FXR/NR1H4 Antibody (PP-A9033A-00) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using PP-A9033A-00:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 02/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23056173
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23056173

Details

Detection NotesSee PMID 23056173

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23056173
  5
reviewed by:
Verified Customer
WB Human 02/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23530060
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23530060

Details

Detection NotesSee PMID 23530060

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23530060

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Verified Customer
02/25/2015
Application: WB
Species: Human

 
Verified Customer
02/25/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NR1H4