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Amidation Bioinformatics

Amidation is a type of post-translational modification (PTM) in which an amide group (-NH2) is added to the C-terminal of an amino acid following translation. Amidation is an essential PTM for neuropeptides and hormones.

Amidation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Amidation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1579 products for the study of Amidation that can be applied to Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP1-46535
Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY licence.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

13 Publications
H00002520-P01
SDS-Page: Recombinant Human Gastrin peptide Protein [H00002520-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

2 Publications
NBP1-81838
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
NBP1-86951
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Analysis in human placenta and testis tissues. Corresponding TGM2 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Staining of human endometrium, fallopian tube, placenta and testis using Anti-TGM2 antibody NBP1-86951 (A) shows similar protein distribution across tissues to independent antibody NBP1-86952 (B).

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

2 Publications
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

10 Publications
NBP2-33680
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human placenta shows no positivity in trophoblastic cells as expected.Immunohistochemistry: Amylin Antibody [NBP2-33680] - Staining of human pancreas shows distinct cytoplasmic positivity in islets of Langerhans.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP2-34075
Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Staining in human heart muscle and lymph node tissues using anti-PAM antibody. Corresponding PAM RNA-seq data are presented for the same tissues.Western Blot: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

AF6108
Adrenomedullin/ADM was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Adrenomedullin/ADM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6108) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications IHC

     1 Review

MAB1417
Insulin was detected in immersion fixed  beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

14 Publications
AF6380
Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Mouse
Applications WB

AF3587
<P align=left>A172 human glioblastoma cell line was stained with Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3587, filled histogram) or control antibody (Catalog # <A class=NoLineLink href=<P align=left>Carboxypeptidase E/CPE was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, IHC, IP

1 Publication
KGE007
 Substance P [Biotin] Substance P [Biotin]


Species Multi-Species
Applications ELISA

     1 Review

31 Publications
NBP2-46349
Western Blot: VIP Antibody (1E8) [NBP2-46349] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse Monoclonal
Species Human
Applications WB, IHC

H00005122-M02
Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
NB100-2803
Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] -  Staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry-Paraffin: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NB100-97841
Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.

Rabbit Polyclonal
Species Human
Applications IP (-), WB

NBP2-62664
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human hypothalamus shows strong positivity in neuronal projections.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB120-14817

Mouse Monoclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

     1 Review

8 Publications
NBP3-04850
Western Blot: GHRH Antibody [NBP3-04850] - Analysis of extracts of various cell lines, using GHRH antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

NBP2-94348
Western Blot: Thyrotropin Releasing Hormone Antibody [NBP2-94348] - Analysis of extracts of various cell lines, using Thyrotropin Releasing Hormone at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.Immunocytochemistry/Immunofluorescence: Thyrotropin Releasing Hormone Antibody [NBP2-94348] - Analysis of H9C2 cells using TRH Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF