Amidation: PTMs Bioinformatics

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Amidation Bioinformatics

Amidation is a type of post-translational modification (PTM) in which an amide group (-NH2) is added to the C-terminal of an amino acid following translation. Amidation is an essential PTM for neuropeptides and hormones.

Amidation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Amidation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1153 products for the study of Amidation that can be applied to Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence from our catalog of antibodies and ELISA kits.

NBP2-34075
Western Blot: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG spImmunocytochemistry/Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Western blot analysis of POMC using NB100-1533 at 0.01ug/ml in NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Analysis in human Anterior Pituitary Gland shows vesilulate staining in glandular cells.

Goat Polyclonal
Species Human, Mouse, Porcine
Applications WB, IHC, IHC-Fr

6 Publications
NB100-97841
Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.

Rabbit Polyclonal
Species Human
Applications IP (-), WB

H00002520-P01
Other Recombinant Human Gastrin peptide Protein


Species Human

2 Publications
NBP2-24470
Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of extracts from K562 cells using Transglutaminase 2 antibody at 1:100.Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Formalin-fixed, paraffin-embedded human placenta  stained with TG2 antibody at 1 ug/ml. Note cytoplasmic staining of trophoblastic cells.

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC-P

NBP1-46535

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

6 Publications
NBP1-35641
Western Blot: Cholecystokinin Antibody [NBP1-35641] BrainImmunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP1-35641] - Human Stomach

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

AF3587
<P align=left>Carboxypeptidase E/CPE was detected in immersion fixed frozen sections of mouse embryo (9.5 d.p.c.) using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=<P align=left>A172 human glioblastoma cell line was stained with Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3587, filled histogram) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, IHC, IP

NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)

Mouse Monoclonal
Species Human, Mouse, Porcine
Applications WB, Flow, ICC/IF

     3 Reviews

NB100-62241

Sheep Polyclonal
Species Human
Applications ELISA

NB300-187
Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).

Guinea Pig Polyclonal
Species Human, Mouse, Rat
Applications ICC/IF, IHC

7 Publications
NBP1-81838
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
AF6108
Adrenomedullin/ADM was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Adrenomedullin/ADM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6108) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications IHC

NB100-2803
Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - (1ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Goat Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

2 Publications
H00005122-M02
Western Blot: PC1/3 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.Immunocytochemistry/Immunofluorescence: PC1/3 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
NB600-713
Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713] - Human pancreas with Mouse anti Human Amylin Peptide, showing labeling of Islet of Langerhans.

Mouse Monoclonal
Species Human
Applications ICC/IF, IHC, IHC-Fr

6 Publications
NBP2-34014
Immunohistochemistry-Paraffin: Thyrotropin Releasing Hormone Antibody [NBP2-34014] - Staining of human hypothalamus shows strong positivity neuronal processes and subsets of neuronal cells and.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP2-29915
Western Blot: GHRH Antibody [NBP2-29915] - Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GHRH antibody detected the GHRH protein (arrow).Immunocytochemistry/Immunofluorescence: GHRH Antibody [NBP2-29915] - Confocal immunofluorescent analysis of GHRH Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC-P

NBP1-05163
Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.Immunohistochemistry-Paraffin: VIP Antibody (2)  - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue. Dilution: 1:100

Mouse Monoclonal
Species Human, Rat, Porcine
Applications WB, ELISA, ICC/IF

NBP1-05164
Immunohistochemistry-Paraffin: CGRP1 Antibody (5) [NBP1-05164] - Paraffin embedded rat tissue stained with NBP1-05164 Anti- alpha CGRP diluted 1:400 in 1% BSA/PBS and incubated over night.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

     1 Review

2 Publications