Insulin Antibody (E2-E3 (same as INS04))

Images

 
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer ...read more
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron ...read more
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
E2-E3 (same as INS04)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Insulin Antibody (E2-E3 (same as INS04)) Summary

Immunogen
Purified pig insulin, conjugated to KLH
Localization
Cytoplasmic
Marker
beta-Cell & Insulinoma Marker
Specificity
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
INS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 5 Reviews rated 4.8
using
NBP2-34260 in the following applications:

Publications
Read Publications using
NBP2-34260 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-34611).

Alternate Names for Insulin Antibody (E2-E3 (same as INS04))

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
DLP00
Species: Hu
Applications: ELISA
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
NBP2-12793
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
DRP300
Species: Hu
Applications: ELISA
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
NBP2-37447
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
NBP1-49533
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
NBP1-90927
Species: Hu
Applications: IHC, IHC-P, WB
DCRP00
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
NBP2-22106
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
D6050
Species: Hu
Applications: ELISA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P

Publications for Insulin Antibody (NBP2-34260)(3)

Reviews for Insulin Antibody (NBP2-34260) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-34260:
Filter by Applications
ICC
(2)
WB
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 Insulin NBP2-34260
Enlarge
4
reviewed by:
Verified Customer
Human 01/20/2019
View

Summary

Sample TestedAdult pancreas
SpeciesHuman
LotI don't have it any more

Comments

CommentspH 9 antigen retrieval
Immunocytochemistry Insulin NBP2-34260
Enlarge
5
reviewed by:
Verified Customer
ICC Human 10/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedpancrea cells
SpeciesHuman
Lot3630-2P 160217
  5
reviewed by:
Ajay Ashok
WB Mouse 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse Pancreas
SpeciesMouse

Comments

CommentsWorks very well at a 1:1000 with sample protein of 40ug
Immunocytochemistry Insulin NBP2-34260
Enlarge
5
reviewed by:
Ajay Ashok
ICC Mouse 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse retina
SpeciesMouse
Western Blot Insulin NBP2-34260
Enlarge
5
reviewed by:
Ajay Ashok
WB Human 06/07/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Insulin Antibody (NBP2-34260). (Showing 1 - 1 of 1 FAQs).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34611AF350
Alexa Fluor 405 NBP2-34611AF405
Alexa Fluor 488 NBP2-34611AF488
Alexa Fluor 532 NBP2-34611AF532
Alexa Fluor 594 NBP2-34611AF594
Alexa Fluor 647 NBP2-34611AF647
Alexa Fluor 700 NBP2-34611AF700
Alexa Fluor 750 NBP2-34611AF750
Biotin NBP2-34611B
DyLight 350 NBP2-34611UV
DyLight 405 NBP2-34611V
DyLight 488 NBP2-34611G
DyLight 550 NBP2-34611R
DyLight 594 NBP2-34611DL594
DyLight 650 NBP2-34611C
DyLight 680 NBP2-34611FR
DyLight 755 NBP2-34611IR
FITC NBP2-34611F
HRP NBP2-34611H
Janelia Fluor 549 NBP2-34611JF549
Janelia Fluor 646 NBP2-34611JF646

Additional Insulin Products

Bioinformatics Tool for Insulin Antibody (NBP2-34260)

Discover related pathways, diseases and genes to Insulin Antibody (NBP2-34260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Insulin Antibody (NBP2-34260)

Discover more about diseases related to Insulin Antibody (NBP2-34260).
 

Pathways for Insulin Antibody (NBP2-34260)

View related products by pathway.

PTMs for Insulin Antibody (NBP2-34260)

Learn more about PTMs related to Insulin Antibody (NBP2-34260).
 

Research Areas for Insulin Antibody (NBP2-34260)

Find related products by research area.

Blogs on Insulin. Showing 1-10 of 13 blog posts - Show all blog posts.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

Inhibiting incretin GIP hormone activity in mouse and monkey models to combat obesity
By Jamshed Arslan, Pharm. D., PhD. We live in a world where 39% of adults are overweight. Our meals trigger the secretion of various gut-derived metabolic hormones called incretins. Fats and carbohydrates in our die...  Read full blog post.

Insulin signaling in brain’s subfornical organ is crucial for regulating cardiometabolic profile
By Jamshed Arslan, Pharm. D., PhD. The hypothalamus is an insulin receptor-rich brain region. Insulin receptor signaling in the CNS can regulate blood pressure, for example, by increasing sympathetic outflow to th...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in ...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Verified Customer
01/20/2019
Application:
Species: Human

Verified Customer
10/24/2017
Application: ICC
Species: Human

 
Ajay Ashok
06/13/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol INS
Uniprot