FoxP3 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

FoxP3 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human FoxP3 isoform 1
  Gln105-Lys200
  Accession # Q9BZS1
• Specificity: Detects human FoxP3 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human FoxD3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXP3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP3 Antibody [Biotin]

• AIID
• AIIDMGC141961
• DIETER
• forkhead box P3
• Forkhead Box Protein P3
• FoxP3
• FOXP3delta7
• immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
• IPEX
• JM2
• MGC141961
• MGC141963
• PIDX
• PIDXMGC141963
• SCURFIN
• XPID
• XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxP3 Antibody (BAF3240)(1)

Publication using BAF3240

• Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L Antigen-induced regulatory T cells in HBV chronically infected patients. Virology, 2007-07-23;368(1):41-9. 2007-07-23 [PMID: 17643462] (Flow Cytometry, Human)

FAQs for FoxP3 Antibody (BAF3240). (Showing 1 - 3 of 3 FAQ).

1. Does FoxP3 antibodies comes in lyophilized form?
   • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
2. What the theoretical molecular weight for FoxP3 antibodies?
   • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
3. What is the immunogen sequence of this FoxP3 antibody?
   • A sequence from Gln105 - Lys200.

Bioinformatics

• Gene Symbol: FOXP3
• Uniprot:
  • Q9BZS1()