FoxP3 Antibody (FXP3/197)

Immunohistochemistry-Paraffin: FoxP3 Antibody (FXP3/197) [NBP2-34282] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FOXP3 Monoclonal Antibody (FXP3/197).

Immunohistochemistry-Paraffin: FoxP3 Antibody (FXP3/197) [NBP2-34282] - Formalin-fixed, paraffin-embedded human Tonsil stained with FOXP3 Monoclonal Antibody (FXP3/197).

• Reactivity: Hu, Mu, Pm
• Applications: Flow, ICC/IF, IHC-P, IP
• Clone: FXP3/197
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FoxP3 Antibody (FXP3/197) Summary

• Immunogen: Full-length human FOXP3 protein
• Localization: Nuclear and Cytoplasmic
• Specificity: Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXP3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
• Application Notes: ELISA (For coating, order NBP2-34620 (BSA free version of NBP2-34282))
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS with 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for FoxP3 Antibody (FXP3/197)

• AIID
• AIIDMGC141961
• DIETER
• forkhead box P3
• Forkhead Box Protein P3
• FoxP3
• FOXP3delta7
• immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
• IPEX
• JM2
• MGC141961
• MGC141963
• PIDX
• PIDXMGC141963
• SCURFIN
• XPID
• XPIDpolyendocrinopathy, enteropathy, X-linked

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FoxP3 Antibody (NBP2-34282). (Showing 1 - 3 of 3 FAQ).

1. Does FoxP3 antibodies comes in lyophilized form?
   • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
2. What the theoretical molecular weight for FoxP3 antibodies?
   • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
3. What is the immunogen sequence of this FoxP3 antibody?
   • The full-length sequence of FoxP3 protein is proprietary.

Bioinformatics

• Gene Symbol: FOXP3
• Uniprot:
  • Q9BZS1()