ESRP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-82202] - Analysis in human rectum and skeletal muscle tissues using NBP1-82202 antibody. Corresponding ESRP1 RNA-seq data are presented for the same tissues.

Western Blot: ESRP1 Antibody [NBP1-82202] - Analysis in human cell lines A-431 and HEK293 using Anti-ESRP1 antibody. Corresponding ESRP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-82202] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-82202] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-82202] - Staining of human Fallopian tube shows moderate nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-82202] - Staining of human skeletal muscle shows low expression in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ESRP1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PGSPNSLGYFPTAANLSGVPPQPGTVVRMQGLAYNTGVKEILNFFQGYQYATEDGLIHTNDQARTLPKEWVCI
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 25143390). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ESRP1 Protein (NBP1-82202PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25143390).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ESRP1 Antibody - BSA Free

• epithelial splicing regulatory protein 1
• FLJ20171
• RBM35A
• RMB35A
• RNA binding motif protein 35A
• RNA-binding motif protein 35A
• RNA-binding protein 35A

Background

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 (PubMed 19285943)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ESRP1 Antibody (NBP1-82202)(1)

Publication using NBP1-82202

• Ishii H, Saitoh M, Sakamoto K et al. Epithelial Splicing Regulatory Proteins 1 (ESRP1) and 2 (ESRP2) Suppress Cancer Cell Motility via Different Mechanisms. J Biol Chem 2014-10-03 [PMID: 25143390] (WB, Human)

Bioinformatics

• Gene Symbol: ESRP1