xCT Antibody


Biological Strategies: Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred ...read more
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa with xCT Antibody NB300-318 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP (-)
1.0 mg/ml
Validated by:

Biological Strategies


xCT Antibody Summary

A synthetic peptide made to an N-terminal region of the human xCT protein (between residues 1-50) [UniProt Q9UPY5].
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Simple Western 10 ug/ml
  • Flow Cytometry 2-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 5 u/gml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This xCT antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Flow (PMID: 20028852). Immunoprecipitation is not recommended. In Western blot this antibody recognizes a band at approx. 35 kDa, and in ICC/IF membrane staining was observed in HeLa cells. Permeablization is recommended prior to performing Flow analysis.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
xCT Lysate (NBL1-16185)
Stomach Lysate (NB820-59260)
Control Peptide
xCT Peptide (NB300-318PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
NB300-318 in the following applications:

Read Publications using
NB300-318 in the following applications:

  • 2 publications
  • IHC
    3 publications
  • WB
    25 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21540084).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for xCT Antibody

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • Member 11 (Slc7a11)
  • SLC7A11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT
  • xCTCCBR1


The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc. In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for xCT Antibody (NB300-318)(40)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB300-318 Applications Species
Miladinovic T, Ungard RG, Linher-Melville K et al. Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain Mol Pain May 16 2018 [PMID: 29761734] (WB, Human) WB Human
Hoehne A, James ML, Alam IS, Ronald JA. [18F]FSPG-PET reveals increased cystine/glutamate antiporter (xc-) activity in a mouse model of multiple sclerosis. J Neuroinflammation. 2018 Feb 22 [PMID: 29471880] (WB, Mouse) WB Mouse
Bechard AR, Hamor PU, Schwendt M, Knackstedt LA. The effects of ceftriaxone on cue-primed reinstatement of cocaine-seeking in male and female rats: estrous cycle effects on behavior and protein expression in the nucleus accumbens. Psychopharmacology (Berl.) 2017 Dec 02 [PMID: 29197981] (WB, Rat) WB Rat
Nasca C, Bigio B, Zelli D et al. Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress. Neuron. 2017 Oct 11 [PMID: 29024663] (Mouse) Mouse
Sayin VI, LeBoeuf SE, Singh SX et al. Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer. Elife. 2017 Oct 02 [PMID: 28967864] (WB, Human) WB Human
Bertaux-Skeirik N, Wunderlich M, Teal E et al. CD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium. J. Pathol. 2017 Aug 01 [PMID: 28497484] (Human) Human
LaCrosse AL, O'Donovan SM, Sepulveda-Orengo MT et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J. Neurosci. 2017 Jun 14 [PMID: 28495973] (WB, Rat)

The Novus xCT antibody was used to look at the effect of GLT-1 knockdown on xCT protein levels
WB Rat
Suarez-Pozos E, Martinez-Lozada Z, Mendez-Flores OG et al. Characterization of the cystine/glutamate antiporter in cultured Bergmann glia cells. Neurochem. Int. 2017 Feb 22 [PMID: 28237844] (WB) WB
Martin C, Patel M, Melendez-Ferro M, Sims B. Erythropoietin-induced cytoprotection in intestinal epithelial cells is linked to system Xc(). Exp. Cell Res. 2017 Feb 06 [PMID: 28167131]
Merckx E, Albertini G, Paterka M et al. Absence of system xc(-) on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis J Neuroinflammation Jan 13 2017 [PMID: 28086920]
Show All 40 Publications.

Reviews for xCT Antibody (NB300-318) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-318:
Filter by Applications
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Images Ratings Applications Species Date Details
Western Blot xCT NB300-318
reviewed by:
WB Human 09/18/2018


ApplicationWestern Blot
Sample TestedHuman Cancer Cells
Western Blot xCT NB300-318
reviewed by:
Dan Cojocari
WB Human 12/24/2015


ApplicationWestern Blot
Sample Testedpancreatic cancer cell line lysate


Blocking DetailsLicor Odyssey Blocking buffer, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1000x dilution, overnight incubation at 4C in 0.1% Twin-20 in TBS

Secondary Antibody

Secondary DescriptionLi-cor IRDye® 800CW Donkey anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#LIC-926-32213
Secondary Concentration10,000 dilution


Detection NotesLi-cor Odyssey CLx
Western Blot xCT NB300-318
reviewed by:
WB Mouse 05/01/2013


ApplicationWestern Blot
Sample TestedMouse Whole Brain
CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.


Blocking Details5% Milk in PBTX, 1 hr

Primary Anitbody

Dilution Ratio1:1000, overnight 4 degrees C, in 5% milk/PBTX

Secondary Antibody

Secondary DescriptionAnti-rabbit
Secondary Concentration1:100,000


Detection Notes5 min Bio-rad Clarity ECL, film exposure for 30 minutes, three negative controls, xCT knockouts


CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-318). (Showing 1 - 5 of 5 FAQs).

  1. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a rabbit negative isotype control IGg/serum?
    • I would recommend catalog number NB810-56910 as a rabbit isotype control for NB300-318.
  2. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a peptide to use for a negative control?
    • I regret to inform you that we do not have a blocking peptide available for this antibody at this time.
  3. Is there a blocking peptide available for use with NB300-318?
    • We unfortunately do not carry the blocking peptide to NB300-318.
  4. We ordered some xCT antibody (NB300-318). Is itpossible to know if there is -NH2 residues on this synthetic peptide. If so, how many -NH2 or lysine are there?
    • The immunogen for NB300-318 contains 1 lysine residue.
  5. For xCT antibody [NB300-318], why do you see a band at 35kDa when the predicted MW is ~55kDa and xCT is not known to undergo cleavage?
    • Although the theoretical molecular weight of xCT is ~55kDa, the protein’s amino acid structure, with multiple charged residues, results in its fast migration. The research paper by Liefferinge et al 2016 (The Journal of Comparative Neurology 524:1015-1032) shows the testing of several different xCT antibodies in knockout study and it was confirmed that the protein indeed runs at 35kDa (Fig.2. lane 1&2 lanes are xCT positive and lanes 3&4 are from xCT knockouts).  

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-318AF405
Alexa Fluor 488 Labeled NB300-318AF488
Alexa Fluor 647 Labeled NB300-318AF647
Alexa Fluor 700 Labeled NB300-318AF700
Biotin Labeled NB300-318B
DyLight 405 Labeled NB300-318V
DyLight 488 Labeled NB300-318G
DyLight 550 Labeled NB300-318R
DyLight 650 Labeled NB300-318C
FITC Labeled NB300-318F
HRP Labeled NB300-318H
PE Labeled NB300-318PE

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human

Dan Cojocari
Application: WB
Species: Human

Application: WB
Species: Mouse


Gene Symbol SLC7A11