xCT Antibody - BSA Free


Orthogonal Strategies: Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red ...read more
Biological Strategies: Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was ...read more
Western Blot: xCT Antibody [NB300-318] - Western blot analysis of protein isolated from subcutaneous tumors derived from in vivo growth of the clones relative to WT-derived tumors revealed that xCT levels remained low, ...read more
Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with NB300-318AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with ...read more
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Biological Strategies: Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred ...read more
Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa with xCT Antibody NB300-318 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/mL in HepG2 cell lysates and antibody at 25 ug/mL. Electropherogram image of corresponding ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, Dual ISH-IHC, IP
BSA Free
1.0 mg/ml

Order Details

xCT Antibody - BSA Free Summary

This xCT Antibody was prepared from a synthetic peptide made to an N-terminal region of the human xCT protein (between residues 1-50) [UniProt Q9UPY5].
Immunogen affinity purified
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  • Dual RNAscope ISH-IHC
  • Flow Cytometry 2-3ug/ml. Use reported in scientific literature (PMID 20028852)
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 5 u/gml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1:1000
Application Notes
Immunoprecipitation is not recommended. In Western blot this antibody recognizes a band at approx. 35 kDa, and in ICC/IF membrane staining was observed in HeLa cells. Permeablization is recommended prior to performing Flow analysis.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Human Stomach Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
xCT Protein (NB300-318PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
NB300-318 in the following applications:

Read Publications using
NB300-318 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21540084).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for xCT Antibody - BSA Free

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • SLC7A11
  • solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT


xCT, also called SLC7A11, is the light chain component of the cysteine/glutamate amino acid exchange transporter system Xc (1,2). System Xc is composed of two subunits, the light chain (xCT) and the heavy chain (CD98hc, SLC3A2) and functions by cellular uptake of cysteine in exchange for glutamate in a 1:1 ratio (1,2). The human xCT gene is located on chromosome 4q28.3 and is synthesized as a 12-pass transmembrane protein with both the N- and C-terminals located intracellularly (2, 3). xCT is a 501 amino acids (aa) protein with a theoretical molecular weight of 55.4 kDa (3, 4). xCT expression serves many functional purposes in cells including redox balance, ferroptosis, and chemotherapy or cancer drug resistance (1-3, 5-7). Import of cysteine by xCT plays a role in promoting oxidative stress response as cysteine is a precursor for glutathione synthesis (2, 3, 5-7). Glutathione is a cofactor for ROS-detoxifying enzymes, including glutathione peroxidase (GPX), which help defend from cellular ROS-induced damage (2, 3, 5-7). In addition to its antioxidant role, xCT also utilizes glutathione and GPX to inhibit ferroptosis, which is iron-dependent, non-apoptotic cell-death that occurs with overproduction of lipid hydroperoxides (1-3, 5-7). As cancer cells often experience high oxidative stress, it is understandable that xCT is overexpressed in a variety of cancer types, such as acute myeloid leukemia and breast cancer, and affects cancer growth, invasion, metastasis, and prognosis (1-3, 5-7). xCT expression has also been shown to play a role in glutathione-mediated drug resistance during cancer treatment (1,5,7). However, studies have shown that xCT knockdown results in increased tumor cell death, highlighting its suitability as a druggable target (1,5,7). Specifically, the xCT inhibitors Sulfasalazine, an approved anti-inflammatory drug, and Erastin, a small molecule inhibitor, are potential therapeutic modalities for treating a variety of cancers when used in combination with radiotherapy or immunotherapy (1-3, 5-7).


1. Liu, J., Xia, X., & Huang, P. (2020). xCT: A Critical Molecule That Links Cancer Metabolism to Redox Signaling. Molecular therapy : the journal of the American Society of Gene Therapy. https://doi.org/10.1016/j.ymthe.2020.08.021

2. Koppula, P., Zhang, Y., Zhuang, L., & Gan, B. (2018). Amino acid transporter SLC7A11/xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer. Cancer communications. https://doi.org/10.1186/s40880-018-0288-x

3. Lin, W., Wang, C., Liu, G., Bi, C., Wang, X., Zhou, Q., & Jin, H. (2020). SLC7A11/xCT in cancer: biological functions and therapeutic implications. American journal of cancer research.

4. xCT: Uniprot (Q9UPY5)

5. Koppula, P., Zhuang, L., & Gan, B. (2020). Cystine transporter SLC7A11/xCT in cancer: ferroptosis, nutrient dependency, and cancer therapy. Protein & cell. https://doi.org/10.1007/s13238-020-00789-5

6. Liu, L., Liu, R., Liu, Y., Li, G., Chen, Q., Liu, X., & Ma, S. (2020). Cystine-glutamate antiporter xCT as a therapeutic target for cancer. Cell biochemistry and function. https://doi.org/10.1002/cbf.3581

7. Cui, Q., Wang, J. Q., Assaraf, Y. G., Ren, L., Gupta, P., Wei, L., Ashby, C. R., Jr, Yang, D. H., & Chen, Z. S. (2018). Modulating ROS to overcome multidrug resistance in cancer. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy. https://doi.org/10.1016/j.drup.2018.11.001


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for xCT Antibody (NB300-318)(71)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 71. Show All 71 Publications.
Publications using NB300-318 Applications Species
Yan Y, Teng H, Hang Q et al. SLC7A11 expression level dictates differential responses to oxidative stress in cancer cells Nature communications 2023-06-21 [PMID: 37339981] (IHC-P, Mouse) IHC-P Mouse
Ma Z, Fan Y, Peng Y et al. miR-6315 silencing protects against spinal cord injury through the Smo and anti-ferroptosis pathway Bioscience reports 2023-04-26 [PMID: 36946310] (WB, Rat) WB Rat
Xie Z, Wang X, Luo X et al. Activated AMPK mitigates diabetes-related cognitive dysfunction by inhibiting hippocampal ferroptosis Biochemical pharmacology 2023-01-01 [PMID: 36502872] (WB, Mouse) WB Mouse
Sketriene D, Battista D, Lalert L et al. Compulsive like eating of high fat high sugar food is associated with 'addiction like' glutamatergic dysfunction in obesity prone rats Addiction Biology 2022-09-01 [PMID: 36001420]
Li N, Yi X, He Y et al. Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection International Journal of Biological Sciences 2022-07-05 [PMID: 35844806] (WB, Human) WB Human
Lee J, Chang-Chien G, Lin S et al. 5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration Oxidative Medicine and Cellular Longevity 2022-02-23 [PMID: 35251467] (WB, Mouse) WB Mouse
Yuan F, Sun Q, Zhang S et al. The dual role of p62 in ferroptosis of glioblastoma according to p53 status Cell & bioscience 2022-02-25 [PMID: 35216629] (WB, Human) WB Human
Chen Y, Yi X, Huo B et al. BRD4770 functions as a novel ferroptosis inhibitor to protect against aortic dissection Pharmacological research 2022-02-08 [PMID: 35149187] (WB, Human) WB Human
AkusjArvi SS, Ambikan AT, Krishnan S Et al. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling iScience 2022-01-10 [PMID: 35005552] (FLOW, Human)

Citation using the Alexa Fluor 594 version of this antibody.
FLOW Human
Yuan F, Sun Q, Zhang S Et al. HSP27 protects against ferroptosis of glioblastoma cells Human cell 2021-11-17 [PMID: 34791597]
Show All 71 Publications.

Reviews for xCT Antibody (NB300-318) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-318:
Filter by Applications
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Images Ratings Applications Species Date Details
Western Blot xCT NB300-318
reviewed by:
WB Human 09/18/2018


ApplicationWestern Blot
Sample TestedHuman Cancer Cells
Western Blot xCT NB300-318
reviewed by:
Verified Customer
WB Human 12/24/2015


ApplicationWestern Blot
Sample Testedpancreatic cancer cell line lysate
Western Blot xCT NB300-318
reviewed by:
Elizabeth Langer
WB Mouse 05/01/2013


ApplicationWestern Blot
Sample TestedMouse Whole Brain

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-318). (Showing 1 - 10 of 10 FAQs).

  1. Do you have a slc7a11 antibody that is conjugated, reacts to mouse, works with cell surface staining, does not need permeabilization?
    • SLC7A11 or xCT antibody NB300-318AF594 targets a cytoplasmic region of the protein, so it should work without permeabilization. However, the data does say it was permeabilized with saponin, so we cannot say for certain if it will work without permeabilization.
  2. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a rabbit negative isotype control IGg/serum?
    • I would recommend catalog number NB810-56910 as a rabbit isotype control for NB300-318.
  3. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a peptide to use for a negative control?
    • I regret to inform you that we do not have a blocking peptide available for this antibody at this time.
  4. Is there a blocking peptide available for use with NB300-318?
    • We unfortunately do not carry the blocking peptide to NB300-318.
  5. We ordered some xCT antibody (NB300-318). Is itpossible to know if there is -NH2 residues on this synthetic peptide. If so, how many -NH2 or lysine are there?
    • The immunogen for NB300-318 contains 1 lysine residue.
  6. For xCT antibody [NB300-318], why do you see a band at 35kDa when the predicted MW is ~55kDa and xCT is not known to undergo cleavage?
    • Although the theoretical molecular weight of xCT is ~55kDa, the protein’s amino acid structure, with multiple charged residues, results in its fast migration. The research paper by Liefferinge et al 2016 (The Journal of Comparative Neurology 524:1015-1032) shows the testing of several different xCT antibodies in knockout study and it was confirmed that the protein indeed runs at 35kDa (Fig.2. lane 1&2 lanes are xCT positive and lanes 3&4 are from xCT knockouts).  
  7. Hello, I'm trying to run a western blot with NB300-318. What is the blocking buffer used?
    • For NB300-318 we recommend using 5% milk to block Blocking solution 1X TBST 5% non-fat dry milk
  8. Is NB300-318 suitable for live cell flow cytometry? (is its epitope in the external side of the cells?) I would need it for FACS sorting application
    • The immunogen for this antibody is cytoplamic, so this antibody would not be suitable for live flow.
  9. I am using the xCT antibody NB300-318 for IHC.  I was wondering if you could recommend  a rabbit negative isotype control IGg/serum?
    •  I would recommend NB810-56910 as an isotype control for you
  10. We are wondering if it is possible to know if there is -NH2 residues on this synthetic peptide. If yes, how many -NH2 or lysine are there?
    • The immunogen for NB300-318 contains 1 lysine residue.  

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Array NB300-318

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has ...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human

Verified Customer
Application: WB
Species: Human

Elizabeth Langer
Application: WB
Species: Mouse


Gene Symbol SLC7A11