xCT Antibody


Western Blot: xCT Antibody [NB300-318] - Detection of xCT in total human and mouse stomach lysate, respectively, using NB300-318. 1 minute ECL exposure.
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.
Flow Cytometry: xCT Antibody [NB300-318] - xCT antibody was tested at 1:400 in HepG2 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT in 0.5 mg/ml of Human Stomach Cancer (left) and HepG2 (right) lysate. This experiment was performed under reducing ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP (-)
1.0 mg/ml

xCT Antibody Summary

A synthetic peptide made to an N-terminal region of the human xCT protein (between residues 1-50) [UniProt Q9UPY5].
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Immunoprecipitation (Negative)
  • Western Blot 1:1000
  • Simple Western 10 ug/ml
  • Flow Cytometry 2-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 5 u/gml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This xCT antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Flow (PMID 20028852). Immunoprecipitation is not recommended. In Western blot this antibody recognizes a band at ~55 kDa, and in ICC/IF membrane staining was observed in HepG2 cells. Permeablization is recommended prior to performing Flow analysis.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
NB300-318 in the following applications:

Read Publications using
NB300-318 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • WB
    18 publications

Reactivity Notes

Human, mouse and rat (PMID 21540084).

Alternate Names for xCT Antibody

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • Member 11 (Slc7a11)
  • SLC7A11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCTCCBR1


The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc. In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, MiAr
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Pm
Applications: WB, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP (-)

Publications for xCT Antibody (NB300-318)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB300-318 Applications Species
Pati D, Kelly K, Stennett B et al. Alcohol consumption increases basal extracellular glutamate in the nucleus accumbens core of Sprague-Dawley rats without increasing spontaneous glutamate release. Eur. J. Neurosci. May 21 2016 12:00AM [PMID:27207718] (WB, Rat) WB Rat
Van Liefferinge J, Bentea E, Demuyser T et al. Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed. 2015 Oct 23 [PMID:26494316] (WB, Mouse) WB Mouse
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015 Jul 30 [PMID:26229077] (WB, Human) WB Human
Merckx E, Demuyser T, Bentea E et al. Lack of effect of Theiler's murine encephalomyelitis virus infection on system xc(.). Neurosci. Lett. 2015 Mar 18 [PMID:25796181] (WB, Mouse) WB Mouse
Rao PS, Saternos H, Goodwani S, Sari Y. Effects of ceftriaxone on GLT1 isoforms, xCT and associated signaling pathways in P rats exposed to ethanol Psychopharmacology (Berl.). 2015 Jan 27 [PMID:25619881] (WB, Rat) WB Rat
Aal-Aaboda M, Alhaddad H, Osowik F et al. Effects of (R)-(-)-5-methyl-1-nicotinoyl-2-pyrazoline on glutamate transporter 1 and cysteine/glutamate exchanger as well as ethanol drinking behavior in male, alcohol-preferring rats J. Neurosci. Res. 2015 Jan 19 [PMID:25601490] (WB, Rat) WB Rat
Boutter Jeannette, Huang Yun, Marovca Blerim et al. Image-based RNA interference screening reveals an individual dependence of acute lymphoblastic leukemia on stromal cysteine support. Oncotarget. 2014 Nov 30 [PMID:25415224] (WB) WB
Martin L, Gardner Lb. et al. Stress-induced inhibition of nonsense-mediated RnA decay regulates intracellular cystine transport and intracellular glutathione through regulation of the cystine/glutamate exchanger SLC7A11. Oncogene. 2014 Nov 17 [PMID:25399695] (WB, Human) WB Human
Fontana JM, Mygatt JG, Conant KL, Parsons CH. Kaposi's Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis. Journal of Skin cancer 2/17/2014 [PMID:24701351] (WB, Human) WB Human
Yang Y, Yee D. IGF-I Regulates Redox Status in Breast Cancer Cells by Activating the Amino Acid Transport Molecule xC-. Cancer Res. 2014 Apr 15 [PMID:24686172] (WB, Human) WB Human
Show All 25 Publications.

Reviews for xCT Antibody (NB300-318) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB300-318:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot xCT NB300-318
reviewed by:
WB Human 12/24/2015


ApplicationWestern Blot
Sample Testedpancreatic cancer cell line lysate


Blocking DetailsLicor Odyssey Blocking buffer, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1000x dilution, overnight incubation at 4C in 0.1% Twin-20 in TBS

Secondary Antibody

Secondary DescriptionLi-cor IRDye® 800CW Donkey anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#LIC-926-32213
Secondary Concentration10,000 dilution


Detection NotesLi-cor Odyssey CLx
Western Blot xCT NB300-318
reviewed by:
WB Mouse 05/01/2013


ApplicationWestern Blot
Sample TestedMouse Whole Brain
CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.


Blocking Details5% Milk in PBTX, 1 hr

Primary Anitbody

Dilution Ratio1:1000, overnight 4 degrees C, in 5% milk/PBTX

Secondary Antibody

Secondary DescriptionAnti-rabbit
Secondary Concentration1:100,000


Detection Notes5 min Bio-rad Clarity ECL, film exposure for 30 minutes, three negative controls, xCT knockouts


CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-318). (Showing 1 - 4 of 4 FAQs).

  1. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a rabbit negative isotype control IGg/serum?
    • I would recommend catalog number NB810-56910 as a rabbit isotype control for NB300-318.
  2. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a peptide to use for a negative control?
    • I regret to inform you that we do not have a blocking peptide available for this antibody at this time.
  3. Is there a blocking peptide available for use with NB300-318?
    • We unfortunately do not carry the blocking peptide to NB300-318.
  4. We ordered some xCT antibody (NB300-318). Is itpossible to know if there is -NH2 residues on this synthetic peptide. If so, how many -NH2 or lysine are there?
    • The immunogen for NB300-318 contains 1 lysine residue.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB300-318AF405
Alexa Fluor (R) 488 Labeled NB300-318AF488
Alexa Fluor (R) 647 Labeled NB300-318AF647
Alexa Fluor (R) 700 Labeled NB300-318AF700
Biotin Labeled NB300-318B
DyLight 405 Labeled NB300-318V
DyLight 488 Labeled NB300-318G
DyLight 550 Labeled NB300-318R
DyLight 650 Labeled NB300-318C
FITC Labeled NB300-318F
HRP Labeled NB300-318H
PE Labeled NB300-318PE

Additional xCT Antibody Products

Related Products by Gene

Bioinformatics Tool for xCT Antibody (NB300-318)

Discover related pathways, diseases and genes to xCT Antibody (NB300-318). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for xCT Antibody (NB300-318)

Discover more about diseases related to xCT Antibody (NB300-318).

Pathways for xCT Antibody (NB300-318)

View related products by pathway.

PTMs for xCT Antibody (NB300-318)

Learn more about PTMs related to xCT Antibody (NB300-318).

Research Areas for xCT Antibody (NB300-318)

Find related products by research area.

Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SLC7A11

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB300-318 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought