xCT Antibody

Images

 
Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

xCT Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal region of the human xCT protein (between residues 1-50) [UniProt Q9UPY5].
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC7A11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 10 ug/ml
  • Flow Cytometry 2-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 5 u/gml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This xCT antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Flow (PMID 20028852). Immunoprecipitation is not recommended. In Western blot this antibody recognizes a band at approx. 35 kDa, and in ICC/IF membrane staining was observed in HeLa cells. Permeablization is recommended prior to performing Flow analysis.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
xCT Lysate (NBL1-16185)
Stomach Lysate (NB820-59260)
Control Peptide
xCT Peptide (NB300-318PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-318 in the following applications:

Publications
Read Publications using
NB300-318 in the following applications:

  • 2 publications
  • IHC
    3 publications
  • WB
    21 publications

Reactivity Notes

Human, mouse and rat (PMID 21540084).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for xCT Antibody

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • Member 11 (Slc7a11)
  • SLC7A11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT
  • xCTCCBR1

Background

The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc. In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-318
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP (-)

Publications for xCT Antibody (NB300-318)(35)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(3)
WB
(21)
All Applications
Filter By Species
Human
(14)
Mouse
(6)
Rat
(4)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB300-318 Applications Species
Nasca C, Bigio B, Zelli D et al. Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress. Neuron. 2017 Oct 11 [PMID: 29024663] (Mouse) Mouse
Sayin VI, LeBoeuf SE, Singh SX et al. Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer. Elife. 2017 Oct 02 [PMID: 28967864] (WB, Human) WB Human
Suarez-Pozos E, Martinez-Lozada Z, Mendez-Flores OG et al. Characterization of the cystine/glutamate antiporter in cultured Bergmann glia cells. Neurochem. Int. 2017 Feb 22 [PMID: 28237844] (WB) WB
Martin C, Patel M, Melendez-Ferro M, Sims B. Erythropoietin-induced cytoprotection in intestinal epithelial cells is linked to system Xc(). Exp. Cell Res. 2017 Feb 06 [PMID: 28167131]
Merckx E, Albertini G, Paterka M et al. Absence of system xc(-) on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis J Neuroinflammation Jan 13 2017 [PMID: 28086920]
Kavanaugh G, Williams J, Morris As et al. Utility of [(18)F]FSPG PET to Image Hepatocellular Carcinoma: First Clinical Evaluation in a US Population. Mol Imaging Biol Sep 27 2016 [PMID: 27677886] (IHC) IHC
Polewski MD, Reveron-Thornton RF, Cherryholmes GA et al. Increased Expression of System xc- in Glioblastoma Confers an Altered Metabolic State and Temozolomide Resistance. Mol Cancer Res 2016 Dec [PMID: 27658422]
Linher-Melville K, Nashed MG, Ungard RG et al. Chronic Inhibition of STAT3/STAT5 in Treatment-Resistant Human Breast Cancer Cell Subtypes: Convergence on the ROS/SUMO Pathway and Its Effects on xCT Expression and System xc- Activity. PLoS ONE. Aug 12 2016 [PMID: 27513743] (WB) WB
Slosky LM, BassiriRad NM, Symons AM et al. The cystine/glutamate antiporter system xc- drives breast tumor cell glutamate release and cancer-induced bone pain. Pain. Nov 1 2016 [PMID: 27482630] (Mouse) Mouse
Soria FN, Zabala A, Pampliega O et al. Cystine/glutamate antiporter blockage induces myelin degeneration. Glia 2016 Aug [PMID: 27247047]
Show All 35 Publications.

Reviews for xCT Antibody (NB300-318) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NB300-318:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot xCT NB300-318
Enlarge
reviewed by:
Dan Cojocari
WB Human 12/24/2015
View

Summary

ApplicationWestern Blot
Sample Testedpancreatic cancer cell line lysate
SpeciesHuman
LotH1

Blocking

Blocking DetailsLicor Odyssey Blocking buffer, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1000x dilution, overnight incubation at 4C in 0.1% Twin-20 in TBS

Secondary Antibody

Secondary DescriptionLi-cor IRDye® 800CW Donkey anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#LIC-926-32213
Secondary Concentration10,000 dilution

Details

Detection NotesLi-cor Odyssey CLx
Western Blot xCT NB300-318
Enlarge
reviewed by:
Anonymous
WB Mouse 05/01/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse Whole Brain
SpeciesMouse
LotF-4
CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.

Blocking

Blocking Details5% Milk in PBTX, 1 hr

Primary Anitbody

Dilution Ratio1:1000, overnight 4 degrees C, in 5% milk/PBTX

Secondary Antibody

Secondary DescriptionAnti-rabbit
Secondary Concentration1:100,000

Details

Detection Notes5 min Bio-rad Clarity ECL, film exposure for 30 minutes, three negative controls, xCT knockouts

Comments

CommentsBiorad Bradford Assay was done to make sure that 10mcg of protein were used for each mouse. I thought the antibody was quite easy to use.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-318). (Showing 1 - 4 of 4 FAQs).

  1. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a rabbit negative isotype control IGg/serum?
    • I would recommend catalog number NB810-56910 as a rabbit isotype control for NB300-318.
  2. I am using the xCT antibody, NB300-318, for IHC. I was wondering if you could recommend a peptide to use for a negative control?
    • I regret to inform you that we do not have a blocking peptide available for this antibody at this time.
  3. Is there a blocking peptide available for use with NB300-318?
    • We unfortunately do not carry the blocking peptide to NB300-318.
  4. We ordered some xCT antibody (NB300-318). Is itpossible to know if there is -NH2 residues on this synthetic peptide. If so, how many -NH2 or lysine are there?
    • The immunogen for NB300-318 contains 1 lysine residue.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-318AF405
Alexa Fluor 488 Labeled NB300-318AF488
Alexa Fluor 647 Labeled NB300-318AF647
Alexa Fluor 700 Labeled NB300-318AF700
Biotin Labeled NB300-318B
DyLight 405 Labeled NB300-318V
DyLight 488 Labeled NB300-318G
DyLight 550 Labeled NB300-318R
DyLight 650 Labeled NB300-318C
FITC Labeled NB300-318F
HRP Labeled NB300-318H
PE Labeled NB300-318PE

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Bioinformatics Tool for xCT Antibody (NB300-318)

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Dan Cojocari
12/24/2015
Application: WB
Species: Human

Anonymous
05/01/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SLC7A11
Entrez
Uniprot