ZEB1 Antibody

Images

 
Western Blot: ZEB1 Antibody [NBP1-05987] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. ZEB1 was also immunoprecipitated by rabbit anti-ZEB1 antibody NBP1-05986, which recognizes an upstream epitope. Whole ...read more
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - FFPE section of human breast carcinoma. Used at a dilution of 1:1000 (0.2ug/ml). Detection method: DAB.
Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250.
Simple Western: ZEB1 Antibody [NBP1-05987] - Simple Western lane view shows a specific band for ZEB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

ZEB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1074 and 1124 of human zinc finger E-box binding homeobox 1 using the numbering given in entry NP_110378.3
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Simple Western 1:50
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100 to 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This antibody is useful for western blot and immunoprecipitation. Customers have also reported success in IHC on paraffin tissues, following antigen retrieval with citrate buffer. Gel Super Shift Assays (PMID: 21771782).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in scientific literature (PMID: 24334458).
Positive Control
ZEB1 Lysate (NBL1-18010)
Reviewed Applications
Read 6 Reviews rated 4.8
using
NBP1-05987 in the following applications:

Publications
Read Publications using
NBP1-05987 in the following applications:

  • 2 publications
  • IHC
    4 publications
  • 5 publications
  • 1 publication
  • WB
    14 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28783105).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • DELTAEF1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • TCF8BZP
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZEB1
  • ZFHEP
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein

Background

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-05987
Species: Hu, Mu, Rt
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP

Publications for ZEB1 Antibody (NBP1-05987)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, MiAr, WB.


Filter By Application
ICC/IF
(2)
IHC
(4)
IHC-P
(5)
MiAr
(1)
WB
(14)
All Applications
Filter By Species
Human
(16)
Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NBP1-05987 Applications Species
Acharya KD, Nettles SA, Sellers KJ et al. The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent eNeuro 2017 Sep 28 [PMID: 28955722] (MiAr, Mouse) MiAr Mouse
Lutz G, Jurak I, Kim ET et al. Viral Ubiquitin Ligase Stimulates Selective Host MicroRNA Expression by Targeting ZEB Transcriptional Repressors Viruses 2017 Aug 07 [PMID: 28783105] (WB, Rat) WB Rat
Katsura A, Tamura Y, Hokari S et al. ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol Oncol. 2017 Jun 15 [PMID: 28618162] (WB, Human) WB Human
Yoon C, Cho SJ, Chang KK et al. Role of Rac1 pathway in epithelial-to-mesenchymal transition and cancer stem-like cell phenotypes in gastric adenocarcinoma. Mol. Cancer Res. May 1 2017 [PMID: 28461325] (Human) Human
Wang Y, Xu X, Yu S et al. Systematic characterization of A-to-I RNA editing hotspots in microRNAs across human cancers. Genome Res. Apr 14 2017 [PMID: 28411194] (Human) Human
Sinh ND, Endo K, Miyazawa K, Saitoh M. Ets1 and ESE1 reciprocally regulate expression of ZEB1/ZEB2, dependent on ERK1/2 activity, in breast cancer cells. Cancer Sci. May 1 2017 [PMID: 28247944] (WB, Human) WB Human
Guo E, Wang Z, Wang S. MiR-200c and miR-141 inhibit ZEB1 synergistically and suppress glioma cell growth and migration. Eur Rev Med Pharmacol Sci 2016 Aug [PMID: 27608897]
Yu QC, Song W, Wang D, Zeng YA. Identification of blood vascular endothelial stem cells by the expression of protein C receptor Cell Res. 2016 Jul 01 [PMID: 27364685] (IHC) IHC
Muto Y, Suzuki K, Kato T et al. Heterogeneous expression of zinc-finger E-box-binding homeobox 1 plays a pivotal role in metastasis via regulation of miR-200c in epithelial-mesenchymal transition. Int J Oncol 2016 Sep [PMID: 27315529] (Human) Human
Werden SJ, Sphyris N, Sarkar TR et al. Phosphorylation of serine 367 of FOXC2 by p38 regulates ZEB1 and breast cancer metastasis, without impacting primary tumor growth. Oncogene Jun 13 2016 [PMID: 27292262] (WB, Human) WB Human
Show All 32 Publications.

Reviews for ZEB1 Antibody (NBP1-05987) (6) 4.86

Average Rating: 4.8
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.
We have 6 reviews tested in 3 application: WB, IHC, IHC-P.

Reviews using NBP1-05987:
Filter by Applications
WB
(4)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot ZEB1 NBP1-05987
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reviewed by:
Anonymous
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell lines
SpeciesHuman
LotA1

Comments

CommentsSample: Whole cell lysates of of two cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:2000) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot ZEB1 NBP1-05987
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reviewed by:
Melissa Skibba
WB Mouse 05/23/2017
View

Summary

ApplicationWestern Blot
Sample Testedkidney,Mouse kidney
SpeciesMouse

Comments

CommentsPerformed on whole kidney in mouse with a 1:1000 primary and a 1:5000 secondary dilution.
 
reviewed by:
Ryan Searcy
IHC Mouse 04/18/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver,Mouse Pancreas,Mouse Spleen
SpeciesMouse
 
reviewed by:
Anonymous
WB Human 11/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGreat!

Blocking

Blocking DetailsBlocking Buffer: 5% Milk, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-mouse, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL

Comments

CommentsGreat!
Immunohistochemistry-Paraffin ZEB1 NBP1-05987
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reviewed by:
Kelsey Stockbridge
IHC-P Mouse 05/09/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedliver and spleen
SpeciesMouse
LotA1
CommentsNuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.

Blocking

Blocking DetailsBlocking Buffer: 10% FCS

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Dilution Buffer: 10% FCS in PBS 0.1% tween, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti Rabbit-biotin then streptavidin-HRP, Secondary Ab Dilution: 1:200

Details

Detection NotesDetection Method: ABC system, Positive Control: liver and spleen, Antigen Retrieval: Citrate, 1 hour at 99 degrees
Fixation DetailsSample Preparation: Formalin fixed

Comments

CommentsNuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.
Western Blot ZEB1 NBP1-05987
Enlarge
reviewed by:
Bhishma Amlani
WB Mouse 05/28/2010
View

Summary

ApplicationWestern Blot
Sample TestedMurine Cell Line, Sample Amount: 50ug
SpeciesMouse
LotA1
CommentsVery clean antibody. However, endogenous and exogenous murine Zeb1 is detected at ~100 kDa.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Incubation Dilution Buffer: 5% BSA in TBST, Incubation Time: 16hr, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP conjugated, donkey anti-rabbit, Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#NA934V

Details

Detection NotesECL, Exposure Time: 30sec, Ctrls: Ctrl Construct (lane 1), Overexpression Construct (lane 2), Specific Band: 100kDa

Comments

CommentsVery clean antibody. However, endogenous and exogenous murine Zeb1 is detected at ~100 kDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB1 Antibody (NBP1-05987). (Showing 1 - 2 of 2 FAQs).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
  2. We have an inquiry regarding the molecular weight of NBP1-05987, Anti-ZEB1. In the datasheet and customer review, the band sizes are very different (upper images: greater than 171kDa, under image (customer review): about 100kDa). If you have any idea why such difference occurred, could you let us know?
    • ZEB1 protein is 1,117 amino acids long and its expected molecular weight is 122.5 kDa. However, because it can undergo multiple types of potential post-translational modifications (including Isopeptide bond, Phosphoprotein, Ubl conjugation, see: Q64318), its observed molecular weight is always different than the predicted one. In our validation studies we observed specific bands in human and mouse cells at approximately 171kDa and almost all of the commercially available antibodies detect the target bands in this range (or at approximately 200kD). This product has been cited in the Journal of Biological Chemistry by Kizuka et al 2011 and they found our antibody to detect the target band at approximately 170kD (Please refer to Supplementary Figure # 3; JBC September 9, 2011 vol. 286 no. 36 31875-31884).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
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Beta Catenin in Cell Adhesion and T-cell Signaling
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Recent Reviews

4.8
5
5
4
1
3
0
2
0
1
0

Anonymous
11/12/2017
Application: WB
Species: Human

Melissa Skibba
05/23/2017
Application: WB
Species: Mouse

 
Ryan Searcy
04/18/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol ZEB1
Entrez
Uniprot