Immunohistochemistry: ZEB1 Antibody [NBP1-05987] - Immunohistochemical staining results of EMT regulators in HG-PanIN: SNAIL, SLUG, TWIST1, and ZEB1. We categorized and defined three grades: grade 0, <10% positive ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Independent Antibodies: Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more
The immunogen recognized by this antibody maps to a region between residue 1074 and 1124 of human zinc finger E-box binding homeobox 1 using the numbering given in entry NP_110378.3
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB1
Purity
Immunogen affinity purified
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Customers have also reported success in IHC on paraffin tissues, following antigen retrieval with citrate buffer. Gel Super Shift Assays (PMID: 21771782). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in scientific literature (PMID: 24334458). Use in microarray reported in scientific literature (PMID: 28955722).Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29744893). ZEB1antibody validated for IHC-P, WB, IHC from verified customer reviews. Knockdown validation (PMID: 31776338).
ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Saforo DJO Characterizing the role of extracellular matrix in non-small cell lung carcinoma metastasis using a 3D microenvironment mimetic in-vitro culture system. Thesis 2019 Aug (WB, Human)
Sample: Whole cell lysates of of two cancer cell lines (10ug) Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature Primary Antibody diluted (1:2000) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Nuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.
Secondary Ab: anti Rabbit-biotin then streptavidin-HRP, Secondary Ab Dilution: 1:200
Details
Detection Notes
Detection Method: ABC system, Positive Control: liver and spleen, Antigen Retrieval: Citrate, 1 hour at 99 degrees
Fixation Details
Sample Preparation: Formalin fixed
Comments
Comments
Nuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.
FAQs for ZEB1 Antibody (NBP1-05987). (Showing 1 - 2 of 2 FAQs).
In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
We have an inquiry regarding the molecular weight of NBP1-05987, Anti-ZEB1. In the datasheet and customer review, the band sizes are very different (upper images: greater than 171kDa, under image (customer review): about 100kDa). If you have any idea why such difference occurred, could you let us know?
ZEB1 protein is 1,117 amino acids long and its expected molecular weight is 122.5 kDa. However, because it can undergo multiple types of potential post-translational modifications (including Isopeptide bond, Phosphoprotein, Ubl conjugation, see: Q64318), its observed molecular weight is always different than the predicted one. In our validation studies we observed specific bands in human and mouse cells at approximately 171kDa and almost all of the commercially available antibodies detect the target bands in this range (or at approximately 200kD). This product has been cited in the Journal of Biological Chemistry by Kizuka et al 2011 and they found our antibody to detect the target band at approximately 170kD (Please refer to Supplementary Figure # 3; JBC September 9, 2011 vol. 286 no. 36 31875-31884).
Bioinformatics Tool for ZEB1 Antibody (NBP1-05987)
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Diseases for ZEB1 Antibody (NBP1-05987)
Discover more about diseases related to ZEB1 Antibody (NBP1-05987).
Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post.
CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post.
Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi... Read full blog post.
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