ZEB1 Antibody

Images

 
Immunohistochemistry: ZEB1 Antibody [NBP1-05987] - Immunohistochemical staining results of EMT regulators in HG-PanIN: SNAIL, SLUG, TWIST1, and ZEB1. We categorized and defined three grades: grade 0, <10% positive ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Independent Antibodies: Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP, MiAr, ChIP, KD, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

ZEB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1074 and 1124 of human zinc finger E-box binding homeobox 1 using the numbering given in entry NP_110378.3
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Microarray
  • Simple Western 1:50
  • Single Cell Western 100 ug/ml
  • Western Blot 1:2000-1:10000
Application Notes
Customers have also reported success in IHC on paraffin tissues, following antigen retrieval with citrate buffer. Gel Super Shift Assays (PMID: 21771782). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in scientific literature (PMID: 24334458). Use in microarray reported in scientific literature (PMID: 28955722).Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29744893). ZEB1antibody validated for IHC-P, WB, IHC from verified customer reviews. Knockdown validation (PMID: 31776338).
Control
ZEB1 Overexpression Lysate
Reviewed Applications
Read 6 Reviews rated 4.8
using
NBP1-05987 in the following applications:

Publications
Read Publications using
NBP1-05987 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28783105).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • DELTAEF1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • TCF8BZP
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZEB1
  • ZFHEP
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein

Background

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82991
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37578
Species: Hu
Applications: ELISA, Flow, ICC/IF, WB
NBP2-03886
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-45831
Species: Hu, Mu, Rt
Applications: IHC, WB
NB100-2450
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NB100-55266
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37364
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
AF3639
Species: Hu
Applications: ChIP, ICC, ICFlow, WB
NBP1-48309
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP2-13857
Species: Hu
Applications: IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
H00001487-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-05987
Species: Hu, Mu, Rt
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP, MiAr, ChIP, KD, Single-Cell Western

Publications for ZEB1 Antibody (NBP1-05987)(83)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: Chemotaxis, GS, ICC/IF, IF, IF/IHC, IHC, IHC-P, KD, MiAr, SW, WB.


Filter By Application
Chemotaxis
(1)
GS
(1)
ICC/IF
(7)
IF
(1)
IF/IHC
(8)
IHC
(1)
IHC-P
(10)
KD
(1)
MiAr
(2)
SW
(1)
WB
(41)
All Applications
Filter By Species
Human
(49)
Mouse
(20)
Rat
(2)
All Species
Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using NBP1-05987 Applications Species
Poyil PK, Siraj AK, Padmaja D et al. Overexpression of the pro-protein convertase furin predicts prognosis and promotes papillary thyroid carcinoma progression and metastasis through RAF/MEK signaling Molecular oncology 2023-02-17 [PMID: 36799665]
Fujiwara S, Saiki Y, Fukushige S et al. TWIST1 is a prognostic factor for neoadjuvant chemotherapy for patients with resectable pancreatic cancer: a preliminary study Surgery today 2023-02-10 [PMID: 36764935] (IHC, Human) IHC Human
Schuhwerk H, Kleemann J, Gupta P et al. The EMT transcription factor ZEB1 governs a fitness-promoting but vulnerable DNA replication stress response Cell reports 2022-12-13 [PMID: 36516781]
Ursu R, Sopel N, Ohs A et al. Glomerular Endothelial Cell-Derived miR-200c Impairs Glomerular Homeostasis by Targeting Podocyte VEGF-A International journal of molecular sciences 2022-12-01 [PMID: 36499397] (ICC/IF, Human)

Details:
Dilution used in ICC/IF 1:100
ICC/IF Human
Han BS, Jung KH, Lee JE et al. Lidocaine enhances the efficacy of palbociclib in triple-negative breast cancer American journal of cancer research 2022-07-15 [PMID: 35968350] (WB, Human) WB Human
Ichikawa MK, Endo K, Itoh Y et al. Ets family proteins regulate the EMT transcription factors Snail and ZEB in cancer cells FEBS open bio 2022-04-22 [PMID: 35451213] (WB, Human) WB Human
Yoshioka H, Suzuki A, Iwaya C, Iwata J Suppression of microRNA 124-3p and microRNA 340-5p ameliorates retinoic acid-induced cleft palate in mice Development (Cambridge, England) 2022-05-01 [PMID: 35420127]
Choi SH, Kim JK, Chen CT et al. KRAS Mutants Upregulate Integrin beta4 to Promote Invasion and Metastasis in Colorectal Cancer Molecular cancer research : MCR 2022-04-08 [PMID: 35394541] (WB) WB
Kang E, Jung SC, Nam SK et al. Tissue miR-200c-3p and circulating miR-1290 as potential prognostic biomarkers for colorectal cancer Scientific reports 2022-02-10 [PMID: 35145164] (MiAr, Human) MiAr Human
Celik M, Celik ZE Role of the Immunohistochemical ZEB1 Expression in Uterine Mesenchymal Neoplasms International journal of surgical pathology 2022-01-07 [PMID: 34994578] (IF/IHC) IF/IHC
Show All 83 Publications.

Reviews for ZEB1 Antibody (NBP1-05987) (6) 4.86

Average Rating: 4.8
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-05987:
Filter by Applications
WB
(4)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Prabir Chakraborty
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell lines
SpeciesHuman
LotA1

Comments

CommentsSample: Whole cell lysates of of two cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:2000) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 05/23/2017
View

Summary

ApplicationWestern Blot
Sample Testedkidney,Mouse kidney
SpeciesMouse

Comments

CommentsPerformed on whole kidney in mouse with a 1:1000 primary and a 1:5000 secondary dilution.
  5
reviewed by:
Verified Customer
IHC Mouse 04/18/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver,Mouse Pancreas,Mouse Spleen
SpeciesMouse
  5
reviewed by:
Hsin-Ling Hsu
WB Human 11/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
Immunohistochemistry-Paraffin ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 05/09/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedliver and spleen
SpeciesMouse
LotA1
Western Blot ZEB1 NBP1-05987
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 05/28/2010
View

Summary

ApplicationWestern Blot
Sample TestedMurine Cell Line, Sample Amount: 50ug
SpeciesMouse
LotA1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB1 Antibody (NBP1-05987). (Showing 1 - 2 of 2 FAQs).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
  2. We have an inquiry regarding the molecular weight of NBP1-05987, Anti-ZEB1. In the datasheet and customer review, the band sizes are very different (upper images: greater than 171kDa, under image (customer review): about 100kDa). If you have any idea why such difference occurred, could you let us know?
    • ZEB1 protein is 1,117 amino acids long and its expected molecular weight is 122.5 kDa. However, because it can undergo multiple types of potential post-translational modifications (including Isopeptide bond, Phosphoprotein, Ubl conjugation, see: Q64318), its observed molecular weight is always different than the predicted one. In our validation studies we observed specific bands in human and mouse cells at approximately 171kDa and almost all of the commercially available antibodies detect the target bands in this range (or at approximately 200kD). This product has been cited in the Journal of Biological Chemistry by Kizuka et al 2011 and they found our antibody to detect the target band at approximately 170kD (Please refer to Supplementary Figure # 3; JBC September 9, 2011 vol. 286 no. 36 31875-31884).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ZEB1 Products

Bioinformatics Tool for ZEB1 Antibody (NBP1-05987)

Discover related pathways, diseases and genes to ZEB1 Antibody (NBP1-05987). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZEB1 Antibody (NBP1-05987)

Discover more about diseases related to ZEB1 Antibody (NBP1-05987).
 

Pathways for ZEB1 Antibody (NBP1-05987)

View related products by pathway.

PTMs for ZEB1 Antibody (NBP1-05987)

Learn more about PTMs related to ZEB1 Antibody (NBP1-05987).
 

Research Areas for ZEB1 Antibody (NBP1-05987)

Find related products by research area.

Blogs on ZEB1.

Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
5
4
1
3
0
2
0
1
0

Prabir Chakraborty
11/12/2017
Application: WB
Species: Human

Verified Customer
05/23/2017
Application: WB
Species: Mouse

 
Verified Customer
04/18/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol ZEB1
Entrez
Uniprot