ZEB1 Antibody

Images

 
Immunohistochemistry: ZEB1 Antibody [NBP1-05987] - Immunohistochemical staining results of EMT regulators in HG-PanIN: SNAIL, SLUG, TWIST1, and ZEB1. We categorized and defined three grades: grade 0, <10% positive ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: ...read more
Independent Antibodies: Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP, MiAr, ChIP, KD, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

ZEB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1074 and 1124 of human zinc finger E-box binding homeobox 1 using the numbering given in entry NP_110378.3
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Microarray
  • Simple Western 1:50
  • Single Cell Western 100 ug/ml
  • Western Blot 1:2000-1:10000
Application Notes
Customers have also reported success in IHC on paraffin tissues, following antigen retrieval with citrate buffer. Gel Super Shift Assays (PMID: 21771782). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in scientific literature (PMID: 24334458). Use in microarray reported in scientific literature (PMID: 28955722).Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29744893). ZEB1antibody validated for IHC-P, WB, IHC from verified customer reviews. Knockdown validation (PMID: 31776338).
Control
ZEB1 Overexpression Lysate
Reviewed Applications
Read 6 Reviews rated 4.8
using
NBP1-05987 in the following applications:

Publications
Read Publications using
NBP1-05987 in the following applications:

  • 1 publication
  • GS
    1 publication
  • 6 publications
  • IF
    1 publication
  • IHC
    8 publications
  • 10 publications
  • KD
    1 publication
  • 1 publication
  • SW
    1 publication
  • WB
    38 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28783105).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • DELTAEF1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • TCF8BZP
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZEB1
  • ZFHEP
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein

Background

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-05987
Species: Hu, Mu, Rt
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP, MiAr, ChIP, KD, Single-Cell Western

Publications for ZEB1 Antibody (NBP1-05987)(74)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: ChIP, GS, ICC/IF, IF, IHC, IHC-P, KD, MiAr, SW, WB.


Filter By Application
ChIP
(1)
GS
(1)
ICC/IF
(6)
IF
(1)
IHC
(8)
IHC-P
(10)
KD
(1)
MiAr
(1)
SW
(1)
WB
(38)
All Applications
Filter By Species
Human
(44)
Mouse
(20)
Rat
(2)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using NBP1-05987 Applications Species
Celik M, Celik ZE Role of the Immunohistochemical ZEB1 Expression in Uterine Mesenchymal Neoplasms International journal of surgical pathology Jan 7 2022 [PMID: 34994578] (IHC) IHC
Chen YY, Jiang KS, Bai XH et al. ZEB1 Induces Ddr1 Promoter Hypermethylation and Contributes to the Chronic Pain in Spinal Cord in Rats Following Oxaliplatin Treatment Neurochemical research May 25 2021 [PMID: 34032956] (IHC, Rat) IHC Rat
Saforo DJO Characterizing the role of extracellular matrix in non-small cell lung carcinoma metastasis using a 3D microenvironment mimetic in-vitro culture system. Thesis 2019 Aug (WB, Human) WB Human
Wang J, Farkas C, Benyoucef A et al. Interplay between the EMT transcription factors ZEB1 and ZEB2 regulates hematopoietic stem and progenitor cell differentiation and hematopoietic lineage fidelity PLoS biology Sep 22 2021 [PMID: 34550965] (WB, Mouse) WB Mouse
Lu J, Cao LL, Xu Y et al. FOXC1 modulates stem-like cell properties and chemoresistance through Hedgehog and EMT signaling in gastric adenocarcinoma Molecular therapy : the journal of the American Society of Gene Therapy Sep 14 2021 [PMID: 34534693]
Kalogirou C, Linxweiler J, Schmucker P Et al. MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer Nature communications Aug 20 2021 [PMID: 34417456] (WB, Human) WB Human
Wojnarowicz PM, Escolano MG, Huang YH et al. Anti-tumor effects of an ID antagonist with no observed acquired resistance NPJ breast cancer May 24 2021 [PMID: 34031428] (WB, Human) WB Human
Carstens JL, Yang S, Correa de Sampaio P et al. Stabilized epithelial phenotype of cancer cells in primary tumors leads to increased colonization of liver metastasis in pancreatic cancer Cell reports Apr 13 2021 [PMID: 33852841]
Li J, Luco AL, Camirand A et al. Vitamin D regulates CXCL12/CXCR4 and epithelial-to-mesenchymal transition in a model of breast cancer metastasis to lung Endocrinology Mar 9 2021 [PMID: 33693593] (ICC/IF, Mouse) ICC/IF Mouse
Pratheeshkumar P, Siraj AK, Divya SP, et al. CHD4 Predicts Aggressiveness in PTC Patients and Promotes Cancer Stemness and EMT in PTC Cells International journal of molecular sciences Jan 6 2021 [PMID: 33419089] (WB, Human) WB Human
Show All 74 Publications.

Reviews for ZEB1 Antibody (NBP1-05987) (6) 4.86

Average Rating: 4.8
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-05987:
Filter by Applications
WB
(4)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Verified Customer
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell lines
SpeciesHuman
LotA1

Comments

CommentsSample: Whole cell lysates of of two cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:2000) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Melissa Skibba
WB Mouse 05/23/2017
View

Summary

ApplicationWestern Blot
Sample Testedkidney,Mouse kidney
SpeciesMouse

Comments

CommentsPerformed on whole kidney in mouse with a 1:1000 primary and a 1:5000 secondary dilution.
  5
reviewed by:
Adrienne Franks
IHC Mouse 04/18/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver,Mouse Pancreas,Mouse Spleen
SpeciesMouse
  5
reviewed by:
Verified Customer
WB Human 11/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGreat!

Blocking

Blocking DetailsBlocking Buffer: 5% Milk, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-mouse , Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL

Comments

CommentsGreat!
Immunohistochemistry-Paraffin ZEB1 NBP1-05987
Enlarge
5
reviewed by:
Kelsey Stockbridge
IHC-P Mouse 05/09/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedliver and spleen
SpeciesMouse
LotA1
CommentsNuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.

Blocking

Blocking DetailsBlocking Buffer: 10% FCS

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Dilution Buffer: 10% FCS in PBS 0.1% tween, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti Rabbit-biotin then streptavidin-HRP, Secondary Ab Dilution: 1:200

Details

Detection NotesDetection Method: ABC system, Positive Control: liver and spleen, Antigen Retrieval: Citrate, 1 hour at 99 degrees
Fixation DetailsSample Preparation: Formalin fixed

Comments

CommentsNuclear expression in: liver (endothelial and mesenchymal cells) and spleen. Two different antigen retrieval and two dilutions were tested : EDTA and Citrate buffer, antibody dilution: 1:200-1:500- each with Biotin Streptavidin-HRP amplification. Best conditions with Citrate buffer, as antigen retrieval, antibody dilution at 1:500.
Western Blot ZEB1 NBP1-05987
Enlarge
4
reviewed by:
Bhishma Amlani
WB Mouse 05/28/2010
View

Summary

ApplicationWestern Blot
Sample TestedMurine Cell Line, Sample Amount: 50ug
SpeciesMouse
LotA1
CommentsVery clean antibody. However, endogenous and exogenous murine Zeb1 is detected at ~100 kDa.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Incubation Dilution Buffer: 5% BSA in TBST, Incubation Time: 16hr, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP conjugated, donkey anti-rabbit, Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#NA934V

Details

Detection NotesECL, Exposure Time: 30sec, Ctrls: Ctrl Construct (lane 1), Overexpression Construct (lane 2), Specific Band: 100kDa

Comments

CommentsVery clean antibody. However, endogenous and exogenous murine Zeb1 is detected at ~100 kDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB1 Antibody (NBP1-05987). (Showing 1 - 2 of 2 FAQs).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
  2. We have an inquiry regarding the molecular weight of NBP1-05987, Anti-ZEB1. In the datasheet and customer review, the band sizes are very different (upper images: greater than 171kDa, under image (customer review): about 100kDa). If you have any idea why such difference occurred, could you let us know?
    • ZEB1 protein is 1,117 amino acids long and its expected molecular weight is 122.5 kDa. However, because it can undergo multiple types of potential post-translational modifications (including Isopeptide bond, Phosphoprotein, Ubl conjugation, see: Q64318), its observed molecular weight is always different than the predicted one. In our validation studies we observed specific bands in human and mouse cells at approximately 171kDa and almost all of the commercially available antibodies detect the target bands in this range (or at approximately 200kD). This product has been cited in the Journal of Biological Chemistry by Kizuka et al 2011 and they found our antibody to detect the target band at approximately 170kD (Please refer to Supplementary Figure # 3; JBC September 9, 2011 vol. 286 no. 36 31875-31884).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

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Recent Reviews

4.8
5
5
4
1
3
0
2
0
1
0

Verified Customer
11/12/2017
Application: WB
Species: Human

Melissa Skibba
05/23/2017
Application: WB
Species: Mouse

 
Adrienne Franks
04/18/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol ZEB1
Entrez
Uniprot