ALK/CD246 Antibody

Images

 
Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) followed by ...read more
ALK/CD246 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) at 3 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

ALK/CD246 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ALK/CD246
Val19-Ser1038
Accession # Q9UM73
Specificity
Detects human ALK/CD246 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ALK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Positive Control
ALK/CD246 Lysate (NBP2-66244)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALK/CD246 Antibody

  • ALK tyrosine kinase receptor
  • ALK
  • anaplastic lymphoma kinase (Ki-1)
  • Anaplastic lymphoma kinase
  • anaplastic lymphoma receptor tyrosine kinase
  • CD246 antigen
  • CD246
  • EC 2.7.10.1
  • Ki-1
  • NBLST3
  • Tcrz

Background

ALK (Anaplastic Lymphoma Kinase; also CD246) is a 200-220 kDa member of the Insulin receptor subfamily, tyrosine kinase family, protein kinase superfamily of proteins.  It shows restricted expression, being limited to select sympathetic and sensory neurons, endothelial cells and tumor cells.  Although activation of ALK appears to induce cell proliferation, the exact ligand(s) for ALK is unknown.  In a temporally-regulated context, midkine has been suggested to bind to ALK, and ionic Zn has been reported to activate ALK cytoplasmically.  Mature human ALK is a 1602 amino acid (aa) type I transmembrane glycoprotein (SwissProt #:Q9UM73).  It contains a 1020 aa extracellular region (aa 19-1038) that contains one protein-protein MAM domain (aa 264-427), an LDL receptor class A region (aa 437-473), a second MAM domain (aa 480-631) and one utilized phosphorylation site at Ser211.  This is accompanied by a long 561 aa cytoplasmic domain that possesses a protein kinase domain (aa 1116-1392) plus eleven utilized Tyr phosphorylation sites.  There is one potential splice variant that contains a four aa insert after Glu549.  There is also an 80 kDa soluble product that arises from cleavage of the extracellular domain.  Although a third 140 kDa product is frequently seen in SDS-Page, its structural basis is unclear.  Almost 20 distinct ALK fusion products arise from gene translocations involving multiple intracellular molecules.  These seem to involve blocks of aa starting between aa 1050-1060 and continuing to aa 1620.  Over aa 19-1038, human ALK shares 88% aa sequence identity with mouse ALK.      

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALK
Entrez
Uniprot