FGFR2 Antibody (OTI5C5)

Images

 
Western Blot: FGF R2 Antibody (5C5) [NBP2-00932] - Analysis of FGF R2 in HEK293T cells (left, control) and HEK293T cells overexpressing FGF R2 (right) using anti-FGF R2 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (5C5) [NBP2-00932] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.
Flow Cytometry: FGFR2 Antibody (5C5) [NBP2-00932] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FGFR2 antibody, and then analyzed ...read more
Western Blot: FGFR2 Antibody (5C5) [NBP2-00932] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Human breast tissue using anti-FGFR2 mouse monoclonal antibody.
Flow Cytometry: FGFR2 Antibody (5C5) [NBP2-00932] - Analysis of Hela cells, using anti-FGFR2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI5C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FGFR2 Antibody (OTI5C5) Summary

Immunogen
Full length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGFR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Control
FGFR2 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-00932 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FGFR2 Antibody (OTI5C5)

  • BBDS
  • BEK
  • BFR-1
  • CD332 antigen
  • CD332
  • CEK3
  • CFD1
  • craniofacial dysostosis 1
  • EC 2.7.10
  • EC 2.7.10.1
  • ECT1
  • FGF R2
  • FGFR2
  • fibroblast growth factor receptor 2
  • FLJ98662
  • Jackson-Weiss syndrome
  • JWS
  • Keratinocyte growth factor receptorreceptor like 14
  • KGFR
  • KSAM
  • K-sam
  • soluble FGFR4 variant 4
  • TK14
  • TK25

Background

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR2 Antibody (NBP2-00932) (0)

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Review for FGFR2 Antibody (NBP2-00932) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-00932:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot FGFR2 NBP2-00932
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reviewed by:
Anonymous
WB Human 02/05/2016
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Summary

ApplicationWestern Blot
Sample TestedHEK293T cells overexpressed with FGFR2
SpeciesHuman
LotA003

Blocking

Blocking Details1% BSA in PBS buffer room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 dilution in 1% BSA in PBS, incubate 4C overnight

Secondary Antibody

Secondary Descriptionanti-mouse IgG, HRP
Secondary Manufacturer Cat#Novus NB7539
Secondary Concentration1:5000

Details

Detection NotesIn cubation with ECL, develop for 30 seconds.

Comments

CommentsThis antibody works very well in confirming my FGFR2 overexpress cell lines. Very low background, strong signal.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGFR2 Antibody (NBP2-00932) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70731AF350
Alexa Fluor 405 NBP2-70731AF405
Alexa Fluor 488 NBP2-70731AF488
Alexa Fluor 532 NBP2-70731AF532
Alexa Fluor 594 NBP2-70731AF594
Alexa Fluor 647 NBP2-70731AF647
Alexa Fluor 700 NBP2-70731AF700
Alexa Fluor 750 NBP2-70731AF750
Allophycocyanin NBP2-70731APC
Biotin NBP2-70731B
DyLight 350 NBP2-70731UV
DyLight 405 NBP2-70731V
DyLight 488 NBP2-70731G
DyLight 550 NBP2-70731R
DyLight 594 NBP2-70731DL594
DyLight 650 NBP2-70731C
DyLight 680 NBP2-70731FR
DyLight 755 NBP2-70731IR
FITC NBP2-70731F
HRP NBP2-70731H
Janelia Fluor 549 NBP2-70731JF549
Janelia Fluor 646 NBP2-70731JF646
PE NBP2-70731PE
PerCP NBP2-70731PCP

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/05/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol FGFR2
Entrez