Home » Mena » Mena Antibodies » Mena Antibody

Mena Antibody

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Mena Antibody [NBP1-87914] - Analysis using Anti-ENAH antibody NBP1-87914 (A) shows similar pattern to independent antibody NBP1-87915 (B).
Independent Antibodies: Western Blot: Mena Antibody [NBP1-87914] - Analysis using Anti-ENAH antibody NBP1-87914 (A) shows similar pattern to independent antibody NBP1-87915 (B).
Immunocytochemistry/ Immunofluorescence: Mena Antibody [NBP1-87914] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human testis using Anti-ENAH antibody.
Western Blot: Mena Antibody [NBP1-87914] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Independent Antibodies: Western Blot: Mena Antibody [NBP1-87914] - Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. ...read more
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human prostate shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining in human prostate and liver tissues using anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human cerebral cortex, liver, prostate and testis using Anti-ENAH antibody NBP1-87914 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human cerebral cortex using Anti-ENAH antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

100% Guarantee on every product

Mena Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPP
Specificity
Specificity of human, mouse, rat Mena antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENAH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Mena Protein (NBP1-87914PEP)
Publications
Read Publications using
NBP1-87914 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Mena Antibody

  • ENA
  • enabled homolog (Drosophila)
  • ENAH
  • FLJ10773
  • Mena
  • MENANDPP1
  • NDPP1
  • protein enabled homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

VASP Antibody
NBP1-85792
Western Blot: VASP Antibody [NBP1-85792] - Analysis in human cell line HEL.
Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining in human small intestine and skeletal muscle tissues using anti-VASP antibody. Corresponding VASP RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Enah/Vasp-like
Antibody
NBP1-80831
Western Blot: Enah/Vasp-like Antibody [NBP1-80831] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: Enah/Vasp-like Antibody [NBP1-80831] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: Enah/Vasp-like Antibody [NBP1-80831] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-EVL antibody NBP1-80831 (A) shows similar protein distribution across tissues to independent antibody NBP2-38132 (B).
Species: Hu
Applications: WB, IHC, IHC-P
Zyxin Antibody
(692624) [Unconjugate
MAB6977
Western blot shows lysates of Human cervical epithelial carcinoma cell line, PT18 mouse mast/basophil cell line, Neuro‑2A mouse neuroblastoma cell line, L6 rat myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Zyxin at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Zyxin was detected in perfusion fixed frozen sections of rat spinal cord using Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motor neurons and dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Zyxin was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC
WASP [p Ser483, p
Ser484] Antibody
NB100-2307
Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Detection of Human Phospho WASP (S483/S484) by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody NB100-2307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(6 Publications)
Species: Hu
Applications: WB
ACTR2 Antibody
(1B10-C4)
H00010097-M01
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - ACTR2 monoclonal antibody (M01), clone 1B10-C4 Analysis of ACTR2 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Analysis of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Western Blot analysis of ACTR2 expression in A-431 ( Cat # L015V1 ).
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Calreticulin
Antibody
NB600-101
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhus University Hospital.
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells of blood vessels (not the RBCs though) and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.
(20 Publications)
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, DB, EM, ICC/IF, IHC, IHC-P, IP, PA, B/N
SSB Antibody
NBP1-33549
Western Blot: SSB Antibody [NBP1-33549] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SSB Antibody [NBP1-33549] - Analysis of paraformaldehyde-fixed A431, using SSB (NBP1-33549) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SSB Antibody [NBP1-33549] - Paraffin-embedded mouse fore brain. SSB antibody dilution: 1:1000.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
c-Abl Antibody
[Unconjugated]
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, Simple Western
Angiopoietin-like
Protein 2/ANGPTL2 Ant ...
AF1444
Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
Species: Mu
Applications: WB, IHC
AICDA Antibody
NBP1-30955
Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Vinculin Antibody
(hVIN-1)
NB600-1293
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. Image is courtesy of customer review.
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution.
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Primary human osteoblast cells labeled (red), actin was stained with Phalloidin (green) and nuclei stained blue.
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr
Applications: WB, ICC/IF, IHC, IHC-Fr, ICC
N-WASP Antibody
NBP1-82512
Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP1-82512] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues. Corresponding WASL RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
FE65 Antibody
NB110-58360
Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.
Western Blot: FE65 Antibody [NB110-58360] - Protein on HEK 293 cell lysate.
Species: Hu, Mu, Rt
Applications: WB, IP
BAIAP2 Antibody
NBP1-88711
Western Blot: BAIAP2 Antibody [NBP1-88711] - Analysis using Anti-BAIAP2 antibody NBP1-88711 (A) shows similar pattern to independent antibody NBP1-88710 (B).
Immunocytochemistry/Immunofluorescence: BAIAP2 Antibody [NBP1-88711] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BAIAP2 Antibody [NBP1-88711] - Staining of human lymph node.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TRIM21/SSA1
Antibody
NBP1-33548
Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Profilin 1
Antibody (OTI1D5)
NBP2-02577
Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)
Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.
(1 Publication)
Species: Hu, Rt, Ca, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(19 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
CDC42 Antibody
(JJ086-04)
NBP2-67895
Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2
Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, IP
MTTP Antibody
NBP1-62489
Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
(1 Publication)
Species: Hu
Applications: WB
Mena Antibody
NBP1-87914
Mena Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Mena Antibody (NBP1-87914)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species

Reviews for Mena Antibody (NBP1-87914) (0)

There are no reviews for Mena Antibody (NBP1-87914). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mena Antibody (NBP1-87914) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Mena Antibody (NBP1-87914)

Discover related pathways, diseases and genes to Mena Antibody (NBP1-87914). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Mena Antibody (NBP1-87914)

Discover more about diseases related to Mena Antibody (NBP1-87914).
 

Pathways for Mena Antibody (NBP1-87914)

View related products by pathway.

PTMs for Mena Antibody (NBP1-87914)

Learn more about PTMs related to Mena Antibody (NBP1-87914).
 

Research Areas for Mena Antibody (NBP1-87914)

Find related products by research area.

Blogs on Mena.

Check out the latest blog posts on Mena.
Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Mena Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol ENAH