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Mena Antibody

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Western Blot: Mena Antibody [NBP1-87914] - Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Orthogonal Strategies: Western Blot: Mena Antibody [NBP1-87914] - Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: Mena Antibody [NBP1-87914] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and focal adhesion sites. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining in human prostate and liver tissues using anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same ...read more
Western Blot: Mena Antibody [NBP1-87914] - Analysis in human cell line NTERA-2.
Western Blot: Mena Antibody [NBP1-87914] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human prostate shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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Mena Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPP
Specificity
Specificity of human, mouse, rat Mena antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENAH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Mena Protein (NBP1-87914PEP)
Publications
Read Publications using NBP1-87914.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Mena Antibody

  • ENA
  • enabled homolog (Drosophila)
  • ENAH
  • FLJ10773
  • Mena
  • MENANDPP1
  • NDPP1
  • protein enabled homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
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Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Whole cell lysate (50 ug/lane) from U937 and Jurkat cells. Lysates were either untreated, mock treated with phosphatase or treated with phosphatase. Antibody used at 1 ug/ml.
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Mena Antibody
NBP1-87914
Mena Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Mena Antibody (NBP1-87914)(2)

We have publications tested in 2 confirmed species: Human, Mouse.


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Bioinformatics

Gene Symbol ENAH
Entrez