Mena Antibody

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human fallopian tube, liver, prostate and testis using Anti-ENAH antibody (A) NBP1-87914 shows similar protein distribution across tissues to independent antibody NBP1-87915 (B).

Western Blot: Mena Antibody [NBP1-87914] - Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Western Blot: Mena Antibody [NBP1-87914] - Analysis using Anti-ENAH antibody NBP1-87914 (A) shows similar pattern to independent antibody NBP1-87915 (B).

Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87914] - MAL2 overexpression disrupts invadopodia formation. Uninfected Clone 9 cells were labeled for Mena. Arrowheads mark Mena present at the surface in patches. Arrowheads mark Mena present at the far distal tips of protrusions induced by MAL2 expression. Bar = 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/12/2/422), licensed under a CC-BY licence.

Western Blot: Mena Antibody [NBP1-87914] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87914] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and focal adhesion sites. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human liver shows low positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Mena Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENAH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Mena Protein (NBP1-87914PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Mena Antibody

• ENA
• enabled homolog (Drosophila)
• ENAH
• FLJ10773
• Mena
• MENANDPP1
• NDPP1
• protein enabled homolog

Background

ENAH/MENA belongs to the Ena/vasodilator-stimulated phosphoprotein (VASP) family of proteins which play a role in regulating the actin cytoskeleton for the control of cell shape and movement. The Ena/VASP family of actin binding proteins includes ENAH/MENA which is the human homolog of drosophila Ena (enabled), VASP, and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein). Ena/VASP proteins contain an EVH1 domain, a central proline-rich domain, and a terminal EVH2 domain. ENAH/MENA is not expressed in normal breast but has been detected and associated with breast lesions with a high risk of transformation and primary and metastatic breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mena Antibody (NBP1-87914)(5)

Publications using NBP1-87914

• Mishra VS, Kumar N, Raza M, Sehrawat S Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme Oncotarget 2020-12-22 [PMID: 33473259] (WB, ICC/IF, Human)
• LOpez-Coral A, Del Vecchio GJ, Chahine JJ et al. MAL2-Induced Actin-Based Protrusion Formation is Anti-Oncogenic in Hepatocellular Carcinoma Cancers (Basel) 2020-02-12 [PMID: 32059473] (WB, IF/IHC, Human)
• kumar N, Prasad P, Jash E et al. cAMP Regulated EPAC1 Supports Microvascular Density, Angiogenic and Metastatic Properties in a Model of Triple Negative Breast Cancer. Carcinogenesis. 2018-07-05 [PMID: 29982410] (WB, Human)
• Harney AS, Karagiannis GS, Pignatelli J et al. The selective Tie2 inhibitor rebastinib blocks recruitment and function of Tie2Hi macrophages in breast cancer and pancreatic neuroendocrine tumors Mol. Cancer Ther. 2017-08-24 [PMID: 28838996] (Mouse)
• Weidmann MD, Surve CR, Eddy RJ et al. Mena(INV) dysregulates cortactin phosphorylation to promote invadopodium maturation. Sci Rep. 2016-11-08 [PMID: 27824079] (Human)

Bioinformatics

• Gene Symbol: ENAH