ESRP1 Antibody - BSA Free

Western Blot: ESRP1 Antibody [NBP1-76536] - Analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: ESRP1 Antibody - BSA Free [NBP1-76536] - Immunofluorescence of ESRP1 in human colon tissue cells with ESRP1 antibody at 20 ug/mL.

Immunohistochemistry: ESRP1 Antibody - BSA Free [NBP1-76536] - Immunohistochemistry of ESRP1 in human colon tissue with ESRP1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ESRP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ESRP1 Antibody - BSA Free (NBP1-76536) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A. The immunogen is located within amino acids 30 - 80 of RBM35A. Amino Acid Squence: LVRPDQLELTED
• Specificity: RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5 ug/ml
• Control Peptide:

  ESRP1 Peptide (NBP1-76536PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ESRP1 Antibody - BSA Free

• epithelial splicing regulatory protein 1
• FLJ20171
• RBM35A
• RMB35A
• RNA binding motif protein 35A
• RNA-binding motif protein 35A
• RNA-binding protein 35A

Background

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESRP1
• Uniprot:
  • NP_001030087(Human)