E-Cadherin Antibody (EP700Y)

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Product Details

Summary
Product Discontinued
View other related E-Cadherin Primary Antibodies

Order Details


    • Catalog Number
      NB110-56937
    • Availability
      Product Discontinued

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E-Cadherin Antibody (EP700Y) Summary

Immunogen
A synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin was used.
Marker
Epithelial Cell Marker, Adherens Junctions Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDH1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:50
  • Simple Western 1:200
  • Western Blot 1: 1000-10000
Application Notes
In Western blot this antibody detects a band at approximately 135 kDa. This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
E-Cadherin Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-56937 in the following applications:

Publications
Read Publications using
NB110-56937 in the following applications:

  • 13 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Purity
Protein A or G purified

Alternate Names for E-Cadherin Antibody (EP700Y)

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

E-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-56937
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for E-Cadherin Antibody (NB110-56937)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(13)
IHC
(2)
IHC-Fr
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB110-56937 Applications Species
Li J, Luco AL, Camirand A et al. Vitamin D regulates CXCL12/CXCR4 and epithelial-to-mesenchymal transition in a model of breast cancer metastasis to lung Endocrinology Mar 9 2021 [PMID: 33693593] (ICC/IF, Mouse) ICC/IF Mouse
Supp DM, Hahn JM, Combs KA et al. Collagen VII Expression is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Formation in Bilayer Engineered Skin Substitutes Cell Transplant Jul 4 2019 [PMID: 31271052] (IHC-Fr, Mouse) IHC-Fr Mouse
Bu D, Crewe C, Kusminski CM et al. Human endotrophin as a driver of malignant tumor growth JCI Insight Mar 21 2019 [PMID: 30896449] (IHC, Human) IHC Human
Hahn JM, Combs KA, Lloyd CM et al. Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds PLoS ONE Mar 5 2019 [PMID: 30835771] (ICC/IF, Human) ICC/IF Human
Uribe D, Cardona A, Degli Esposti D, Cros MP. Antiproliferative Effects of Epigenetic Modifier Drugs through E-cadherin Up-regulation in Liver Cancer Cell Lines. gene Jul 10 1905 [PMID: 29735783] (ICC/IF) ICC/IF
Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum. Reprod. 2015 Feb 01 [PMID: 25432925] (WB, Human)

Details:
E-Cadherin antibody used for WB on lysates of Ishikawa cells that were cultured for 2 days in serum medium or serum starved medium in the presence or absence of 10 ng/ml IGF1 (Figure 3)
WB Human
Raghunathan Vk, Dreier B, Morgan Jt et al. Involvement of YAP, TAZ and HSP90 in Contact Guidance and Intercellular Junction Formation in Corneal Epithelial Cells. PLoS OnE. 2014 Oct 08 [PMID: 25290150] (ICC/IF, Human) ICC/IF Human
Huang C, Du R, Zhang P et al. Expression, purification, and functional characterization of recombinant PTD-SARA. Acta Biochim Biophys Sin (Shanghai) 2011 Feb [PMID: 21266541] (ICC/IF) ICC/IF
Fanjul M, Gmyr V, Sengenss C et al. Evidence for epithelial-mesenchymal transition in adult human pancreatic exocrine cells. J Histochem Cytochem 2010 Sep [PMID: 20530463] (ICC/IF) ICC/IF
Tarantal AF, Chen H, Shi TT et al. Overexpression of transforming growth factor-beta1 in fetal monkey lung results in prenatal pulmonary fibrosis. Eur Respir J 2010 Oct [PMID: 20351039] (ICC/IF) ICC/IF
Show All 17 Publications.

Review for E-Cadherin Antibody (NB110-56937) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-56937:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Takeshi Shi
IF Human 11/02/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedMcf10A cell line
SpeciesHuman

Product General Protocols

View specific protocols for E-Cadherin Antibody (NB110-56937): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Cadherin Antibody (NB110-56937). (Showing 1 - 2 of 2 FAQs).

  1. Hello. I am currently working on Western Blots of serum with E-Cadherin N-terminus antibodies from other companies. Although a 55kDa splice fragment is not reported in the literature, I continue to obtain this band in my experimental blot, and not in my negative To work this out, I am looking for a monoclonal antibody specific to the N-terminus, and also an available blocking peptide to determine whether the band is specific or not. I am looking at your product EP700Y. It appears is it generated from a peptide immunogen. Is this peptide available for purchase?
    • Unfortunately we do not have this peptide available for purchase or to send out.
  2. I am using the E-cadherin antibody from your company (EP700Y) and the product information sheet indicates that the antibody detects a band at 135 kDa. But in my western I get a band around 100 kDa & other literature sources suggest the same. Can you please clarify this for me? Thank you.
    • E-cadherin is an 882 amino acids long protein with predicted molecular size of 97.4 kDa. However, this protein undergoes different post-translational modifications including glycosylation and the post-translationally modified protein may run at higher than expected molecular weight position. In our WB validation assays, most of our E-Cadherin antibodies including our best-seller #NBP2-19051, detects this target between 100-140 kDa positions. Therefore, we can assure you that your observed band at/above ~100 kDa is a specific band for the full length E-Cadherin protein. Please note that under some situations such as apoptosis or calcium influx, E-Cadherin protein undergoes molecular cleavage and its processing results in three C-terminal fragments (E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3). These cleaved fragments may run below ~40 kDa position in the WB assay (PMID 16417575; PMID 13678586; PMID 11953314).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for E-Cadherin Antibody (NB110-56937)

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Diseases for E-Cadherin Antibody (NB110-56937)

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Blogs on E-Cadherin.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and ...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Takeshi Shi
11/02/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CDH1
Entrez
Uniprot