E-Cadherin Antibody (DECMA-1)

Images

 
Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a ...read more
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.
Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal ...read more
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP, MiAr
Clone
DECMA-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

E-Cadherin Antibody (DECMA-1) Summary

Immunogen
Mouse embryonal carcinoma cell line PCC4 Aza RI.
Localization
Cell membrane; cell-cell junction; single-pass type I membrane protein
Marker
Epithelial Cell Marker, Adherens Junctions Marker
Specificity
Antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CDH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000
  • Immunocytochemistry/Immunofluorescence 1:1600
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in westernblot or immunohistochemical assays.
Control
E-Cadherin Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB120-11512 in the following applications:

Publications
Read Publications using
NB120-11512 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Raw ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for E-Cadherin Antibody (DECMA-1)

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Cadherin Antibody (NB120-11512)(3)

Reviews for E-Cadherin Antibody (NB120-11512) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NB120-11512:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry E-Cadherin NB120-11512
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reviewed by:
Anonymous
ICC Mouse 06/06/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult small intestine
SpeciesMouse
Lot126M4805v
Western Blot E-Cadherin NB120-11512
Enlarge
reviewed by:
Anonymous
WB Other 11/18/2013
View

Summary

ApplicationWestern Blot
Sample Testedcat testis, cat ovary, HEK293 whole cell lysate
SpeciesOther
Lot060m4825

Blocking

Blocking Details5% Blotto in 1xTBST buffer, 1 hour at room temperature on a rocker

Primary Anitbody

Dilution RatioDiluted 1:1250 in 5% Blotto (TBST), incubated overnight at 4C on a rocker

Secondary Antibody

Secondary DescriptionAffinity purified goat anti-rat IgG (H&L) antibody conjugated to horseradish peroxidase.
Secondary Manufacturer Cat#7077s
Secondary Concentration1:5000

Details

Detection NotesDetection was performed using Supersignal West Pico chemiluminescent substrate and vivisualized using a ChemiDoc XRS system

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Cadherin Antibody (NB120-11512) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
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Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
06/06/2019
Application: ICC
Species: Mouse

Anonymous
11/18/2013
Application: WB
Species: Other

Bioinformatics

Gene Symbol CDH1
Entrez
Uniprot