![Western Blot: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Western-Blot-NBP2-54587-img0006.jpg "Western Blot: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 4A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53229). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant human E-Cadherin protein (Uniprot: P12830 ) |
| Localization | Cell Surface |
| Marker | Intercellular Junction Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDH1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Reviewed Applications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Santhosh Sivajothi |
IMC | Human | 09/25/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CDH1 |
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