E-Cadherin Antibody (180224) [Unconjugated]

Images

 
E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 0.5 µg/mL for 1 hour at room temperature ...read more
E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 5 µg/mL for 1 hour at room temperature ...read more
E‑Cadherin was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 3 µg/mL for 3 hours at room temperature. Cells ...read more
Western blot shows lysates of MCF‑7 human breast cancer cell line, BT‑20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
180224
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

E-Cadherin Antibody (180224) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Asp155-Ile707
Accession # P12830
Specificity
Detects human E-Cadherin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDH1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB18381 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody (180224) [Unconjugated]

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB18381
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Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF

Publications for E-Cadherin Antibody (MAB18381)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(3)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB18381 Applications Species
Z Jia, K Bao, P Wei, X Yu, Y Zhang, X Wang, X Wang, L Yao, L Li, P Wu, W Yuan, S Wang, J Zheng, Y Hua, M Hong EGFR activation-induced decreases in claudin1 promote MUC5AC expression and exacerbate asthma in mice Mucosal Immunol, 2020;0(0):. 2020 [PMID: 32132671] (Western Blot, Mouse) Western Blot Mouse
Wada , Naohisa, Maeda , Hidefumi, Hasegawa , Daigaku, Gronthos , Stan, Bartold , Peter Ma, Menicanin , Danijela, Fujii , Shinsuke, Yoshida , Shinichi, Tomokiyo , Atsushi, Monnouchi , Satoshi, Akamine , Akifumi Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev, 2014;23(18):2225-36. 2014 [PMID: 24380401] (ICC, Human) ICC Human
Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (ICC, Human) ICC Human
Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation. Blood, 2009;113(21):5157-66. 2009 [PMID: 19279330] (Flow Cytometry, Human) Flow Cytometry Human
Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T Generation of pluripotent stem cells from adult human testis. Nature, 2008;456(7220):344-9. 2008 [PMID: 18849962] (ICC, Human) ICC Human
Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity. Cancer Res., 2004;64(6):2024-9. 2004 [PMID: 15026339] (IHC-P, Human) IHC-P Human

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Blogs on E-Cadherin.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and ...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Bioinformatics

Gene Symbol CDH1
Uniprot