E-Cadherin Antibody (180224) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human E-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB18381P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 180224
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

E-Cadherin Antibody (180224) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
  Asp155-Ile707
  Accession # P12830
• Specificity: Detects human E-Cadherin in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody (180224) [Phycoerythrin]

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) Science 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Cadherin Antibody (FAB18381P)(10)

Publications using FAB18381P

• Subayyil, AA;Basmaeil, YS;Kulayb, HB;Alrodayyan, M;Alhaber, LAA;Almanaa, TN;Khatlani, T; Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro International journal of molecular sciences 2023-05-31 [PMID: 37298519] (Flow Cytometry, Human)
• Marta Bermejo‐Jambrina, Julia Eder, Tanja M Kaptein, John L van Hamme, Leanne C Helgers, Killian E Vlaming et al. Infection and transmission of SARS‐CoV‐2 depend on heparan sulfate proteoglycans The EMBO Journal 10/18/2021 [PMID: 34510494]
• Yasser Basmaeil, Abdullah Al Subayyil, Mohammad Abumaree, Tanvir Khatlani Conditions Mimicking the Cancer Microenvironment Modulate the Functional Outcome of Human Chorionic Villus Mesenchymal Stem/Stromal Cells in vitro Frontiers in Cell and Developmental Biology 2021-06-21 [PMID: 34235143] (Flow Cytometry, Human)
• EVA JUENGEL, ANITA THOMAS, JOCHEN RUTZ, JASMINA MAKAREVIC, IGOR TSAUR, KAREN NELSON et al. Amygdalin inhibits the growth of renal cell carcinoma cells in vitro International Journal of Molecular Medicine 2/1/2016 [PMID: 26709398]
• EVA JUENGEL, ANITA THOMAS, JOCHEN RUTZ, JASMINA MAKAREVIC, IGOR TSAUR, KAREN NELSON et al. Amygdalin inhibits the growth of renal cell carcinoma cells in vitro International Journal of Molecular Medicine 2016-02-01 [PMID: 26709398]
• Sandy Azzi, Cindy Gallerne, Cristina Romei, Vincent Le Coz, Rosaria Gangemi, Krystel Khawam et al. Human Renal Normal, Tumoral, and Cancer Stem Cells Express Membrane-Bound Interleukin-15 Isoforms Displaying Different Functions1 Neoplasia 7/4/2015 [PMID: 26152359]
• Sefah K, Bae K, Phillips J, Siemann D, Su Z, McClellan S, Vieweg J, Tan W Cell-based selection provides novel molecular probes for cancer stem cells. Int J Cancer, 2013-02-08;132(11):2578-88. 2013-02-08 [PMID: 23150432] (Flow Cytometry, Human)
• V Coppola, M Musumeci, M Patrizii, A Cannistraci, A Addario, M Maugeri-Saccà et al. BTG2 loss and miR-21 upregulation contribute to prostate cell transformation by inducing luminal markers expression and epithelial–mesenchymal transition Oncogene 4/4/2013 [PMID: 22614007]
• Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway. PLoS ONE, 2012-02-21;7(2):e31624. 2012-02-21 [PMID: 22363690] (Flow Cytometry, Human)
• Nakahara M, Saeki K, Nakamura N Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition. Cloning Stem Cells, 2009-03-01;11(1):5-18. 2009-03-01 [PMID: 19090661] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CDH1
• Uniprot:
  • P12830()