E-Cadherin Antibody (114420) [Unconjugated]

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

mlMCD3 cells were stained with Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 114420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

E-Cadherin Antibody (114420) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
  Asp157-Val709 (predicted)
  Accession # P09803
• Specificity: Detects mouse E-Cadherin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CDH1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody (114420) [Unconjugated]

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Cadherin Antibody (MAB7481)(16)

Publications using MAB7481

• JR Enriquez, HA McCauley, KX Zhang, JG Sanchez, GT Kalin, RA Lang, JM Wells A dietary change to a high-fat diet initiates a rapid adaptation of the intestine Cell Reports, 2022-11-15;41(7):111641. 2022-11-15 [PMID: 36384107] (IHC, Mouse, Transgenic Mouse)
• CP Tran, M Scurr, L O'Connor, JN Buzzelli, GZ Ng, SCN Chin, LA Stamp, T Minamoto, AS Giraud, LM Judd, P Sutton, TR Menheniott IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells Oncotarget, 2022-06-01;13(0):785-799. 2022-06-01 [PMID: 35677533] (Flow Cytometry, Mouse)
• PQ Duy, SC Weise, C Marini, XJ Li, D Liang, PJ Dahl, S Ma, A Spajic, W Dong, J Juusola, E Kiziltug, AJ Kundishora, S Koundal, MZ Pedram, LA Torres-Fer, K Händler, E De Domenic, M Becker, T Ulas, SA Juranek, E Cuevas, LT Hao, B Jux, AMM Sousa, F Liu, SK Kim, M Li, Y Yang, Y Takeo, A Duque, C Nelson-Wil, Y Ha, K Selvaganes, SM Robert, AK Singh, G Allington, CG Furey, AT Timberlake, BC Reeves, H Smith, A Dunbar, T DeSpenza, J Goto, A Marlier, A Moreno-De-, X Yu, WE Butler, BS Carter, EMR Lake, RT Constable, P Rakic, H Lin, E Deniz, H Benveniste, NS Malvankar, JI Estrada-Ve, CA Walsh, SL Alper, JL Schultze, K Paeschke, A Doetzlhofe, FG Wulczyn, SC Jin, RP Lifton, N Sestan, W Kolanus, KT Kahle Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus Nature Neuroscience, 2022-04-04;25(4):458-473. 2022-04-04 [PMID: 35379995] (IHC, Mouse)
• AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021-12-01;0(0):. 12/1/2021 [PMID: 34856121]
• R Morikawa, Y Nemoto, Y Yonemoto, S Tanaka, Y Takei, S Oshima, T Nagaishi, K Tsuchiya, K Nozaki, T Mizutani, T Nakamura, M Watanabe, R Okamoto Intraepithelial lymphocytes suppress the intestinal tumor growth by cell-to-cell contact via CD103/E-cadherin signal Cellular and Molecular Gastroenterology and Hepatology, 2021-01-28;0(0):. 2021-01-28 [PMID: 33515805] (IHC, Mouse)
• Kui Li, Zheng Zhou, Ju Li, Rui Xiang miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1 Technol Cancer Res Treat 12/17/2020 [PMID: 33327874]
• Dong Chen, Siyuan Hu, Jie Liu, Shaohua Li E‑cadherin regulates biological behaviors of neural stem cells and promotes motor function recovery following spinal cord injury Experimental and Therapeutic Medicine 1/15/2019 [PMID: 30783478]
• Vladimir Ustiyan, Craig Bolte, Yufang Zhang, Lu Han, Yan Xu, Katherine E. Yutzey et al. FOXF1 transcription factor promotes lung morphogenesis by inducing cellular proliferation in fetal lung mesenchyme Developmental Biology 11/1/2018 [PMID: 30153454]
• Scott A. Rankin, Kyle W. McCracken, David M. Luedeke, Lu Han, James M. Wells, John M. Shannon et al. Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis Developmental Biology 2/1/2018 [PMID: 29217200]
• Scott A. Rankin, Kyle W. McCracken, David M. Luedeke, Lu Han, James M. Wells, John M. Shannon et al. Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis Developmental Biology 2018-02-01 [PMID: 29217200] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties Sci Rep, 2016-11-29;6(0):37914. 2016-11-29 [PMID: 27897208] (Neutralization, Mouse)
• The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation J Bone Miner Res, 2016-10-26;0(0):. 2016-10-26 [PMID: 27218883] (ICC, IHC-P, Mouse)
• Bittencourt F, Wu S, Bridges J, Miller W The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland. J Virol, 2014-08-06;88(20):11811-24. 2014-08-06 [PMID: 25100846] (IHC-Fr, Mouse)
• Zhang , Hanwei, Chin , Arnold I Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis. PLoS ONE, 2014-04-14;9(4):e94793. 2014-04-14 [PMID: 24733360] (IHC-P, Mouse)
• Integrin alpha9beta1 in airway smooth muscle suppresses exaggerated airway narrowing. J. Clin. Invest., 2012-07-09;122(8):2916-27. 2012-07-09 [PMID: 22772469] (IHC-Fr, Mouse)
• Moore RN, Cherry JF, Mathur V E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells. Stem Cells Dev., 2011-06-01;21(1):30-41. 2011-06-01 [PMID: 21469943] (ICC, Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CDH1
• Uniprot:
  • P09803()