E-Cadherin Antibody (114420) [Unconjugated]

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for E‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 114420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

E-Cadherin Antibody (114420) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
  Asp157-Val709 (predicted)
  Accession # P09803
• Specificity: Detects mouse E-Cadherin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CDH1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody (114420) [Unconjugated]

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Cadherin Antibody (MAB7481)(6)

Publications using MAB7481

• Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties Sci Rep, 2016;6(0):37914. 2016 [PMID: 27897208] (Neutralization, Mouse)
• The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation J Bone Miner Res, 2016;0(0):. 2016 [PMID: 27218883] (ICC, Mouse)
• Bittencourt F, Wu S, Bridges J, Miller W The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland. J Virol, 2014;88(20):11811-24. 2014 [PMID: 25100846] (IHC-Fr, Mouse)
• Zhang, Hanwei, Chin, Arnold I Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis. PLoS ONE, 2014;9(4):e94793. 2014 [PMID: 24733360] (IHC-P, Mouse)
• Integrin alpha9beta1 in airway smooth muscle suppresses exaggerated airway narrowing. J. Clin. Invest., 2012;122(8):2916-27. 2012 [PMID: 22772469] (IHC-Fr, Mouse)
• Moore RN, Cherry JF, Mathur V E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells. Stem Cells Dev., 2012;21(1):30-41. 2012 [PMID: 21469943] (ICC, Mouse)

Bioinformatics

• Gene Symbol: CDH1
• Uniprot:
  • P09803()