DAX1/NR0B1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR0B1 protein stained by NR0B1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832] - Cal27 xenograft, using NR0B1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Pm, RM
• Applications: Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DAX1/NR0B1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DAX1/NR0B1. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm
• Predicted Species: Chimpanzee (99%), Rhesus Macaque (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR0B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 1:50
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Human fetal adrenal glands as sample; separated by size; antibody dilution of 1:50; observed molecular weight was ~51 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DAX1/NR0B1 Antibody - BSA Free

• AHC
• AHCDSS
• AHCH
• AHX
• DAX1
• DAX1DAX-1
• dosage-sensitive sex reversal
• DSS-AHC critical region on the X chromosome protein 1
• GTD
• HHG
• NR0B1
• NROB1
• nuclear hormone receptor
• Nuclear receptor DAX-1
• nuclear receptor subfamily 0 group B member 1
• nuclear receptor subfamily 0, group B, member 1

Background

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DAX1/NR0B1 Antibody (NBP1-32832)(1)

Publication using NBP1-32832

• I Savchuk, ML Morvan, JP Antignac, K Gemzell-Da, B LeBizec, O Soder, K Svechnikov Androgenic potential of human fetal adrenals at the end of the first trimester Endocr Connect, 2017-06-07;0(0):. 2017-06-07 [PMID: 28592511] (Simple Western, Human)

Bioinformatics

• Gene Symbol: NR0B1
• Entrez:
  • 190(Human)
• Uniprot:
  • P51843(Human)