![Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize2/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg "Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MID1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for MID1 Antibody (NBP3-05061)Discover more about diseases related to MID1 Antibody (NBP3-05061).
| Pathways for MID1 Antibody (NBP3-05061)View related products by pathway.
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PTMs for MID1 Antibody (NBP3-05061)Learn more about PTMs related to MID1 Antibody (NBP3-05061).
| Research Areas for MID1 Antibody (NBP3-05061)Find related products by research area.
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Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post. |
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| Gene Symbol | MID1 |
![Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg "Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody. Blue: DAPI for nuclear staining.")
![Western Blot: MID1 Antibody [NBP3-05061] - Analysis of extracts of various cell lines, using MID1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize2/MID1 Antibody-Western Blot-NBP3-05061-img0002.jpg "Western Blot: MID1 Antibody [NBP3-05061] - Analysis of extracts of various cell lines, using MID1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Western Blot: EFS Antibody [NBP2-31993] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/images2/EFS-Antibody-Western-Blot-NBP2-31993-img0003.jpg)
![Immunohistochemistry: EFS Antibody [NBP2-31993] - Staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.](http://images.novusbio.com/images2/EFS-Antibody-Immunohistochemistry-NBP2-31993-img0001.jpg)
![Western Blot: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of PGR expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Western-Blot-H00005241-M08-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Immunocytochemistry-Immunofluorescence-H00005241-M08-img0006.jpg)
![Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Immunohistochemistry-Paraffin-H00005241-M08-img0007.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)
![Immunocytochemistry/Immunofluorescence: TMEM37 Antibody [NBP2-47602] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus, nucleoli fibrillar center & cytosol.](http://images.novusbio.com/images2/TMEM37-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47602-img0003.jpg)
![Immunohistochemistry-Paraffin: TMEM37 Antibody [NBP2-47602] - Staining in human kidney and pancreas tissues using anti-TMEM37 antibody. Corresponding TMEM37 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/TMEM37-Antibody-Immunohistochemistry-Paraffin-NBP2-47602-img0006.jpg)
![Immunohistochemistry-Paraffin: TMEM37 Antibody [NBP2-47602] - Staining of human kidney shows high expression.](http://images.novusbio.com/images2/TMEM37-Antibody-Immunohistochemistry-Paraffin-NBP2-47602-img0004.jpg)
![Immunohistochemistry: SH3BP4 Antibody [NBP1-86274] - Staining of human small intestine shows strong luminal membranous positivity in glandular cells.](http://images.novusbio.com/images2/SH3BP4-Antibody-Immunohistochemistry-NBP1-86274-img0004.jpg)
![Inhibition of T-cells: SH3BP4 Antibody [NBP1-86274] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Hep-G2](http://images.novusbio.com/images2/SH3BP4-Antibody-Inhibition-of-T-cells-NBP1-86274-img0005.jpg)
![Western Blot: TTP Antibody (8B5) [NBP2-02105] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTP.](http://images.novusbio.com/images/TTP Antibody (8B5)-Western Blot-NBP2-02105-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TTP Antibody (OTI8B5) [NBP2-02105] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TTP.](http://images.novusbio.com/images2/TTP-Antibody-(8B5)-Immunocytochemistry-Immunofluorescence-NBP2-02105-img0009.jpg)
![Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Human prostate tissue using anti-TTP mouse monoclonal antibody.](http://images.novusbio.com/images/TTP Antibody (8B5)-Immunohistochemistry-Paraffin-NBP2-02105-img0007.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.](http://images.novusbio.com/images2/Myelin-Protein-Zero-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1607-img0001.jpg)
![Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.](http://images.novusbio.com/images2/Myelin-Protein-Zero-Antibody-Immunohistochemistry-NB100-1607-img0002.jpg)
![Western Blot: PMP22 Antibody (JM52-30) [NBP2-67068] - Analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.](http://images.novusbio.com/images2/PMP22 Antibody (JM52-30)-Western Blot-NBP2-67068-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PMP22 Antibody (JM52-30) [NBP2-67068] - Staining PMP22 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/PMP22-Antibody-(JM52-30)-Immunocytochemistry-Immunofluorescence-NBP2-67068-img0002.jpg)
![Immunohistochemistry-Paraffin: PMP22 Antibody (JM52-30) [NBP2-67068] - Analysis of paraffin-embedded rat brain tissue using anti-PMP22 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/PMP22-Antibody-(JM52-30)-Immunohistochemistry-Paraffin-NBP2-67068-img0001.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Western-Blot-H00003845-M01-img0004.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/KRAS Antibody (3B10-2F2)-Western Blot-H00003845-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Immunocytochemistry-Immunofluorescence-H00003845-M01-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")