SOAT1 Antibody

Images

 
There are currently no images for SOAT1 Antibody (NB400-141).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SOAT1 Antibody Summary

Immunogen
A fusion protein construct that contains the N-terminal region of human SOAT1. [UniProt# P35610]
Localization
Endoplasmic reticulum, membrane
Specificity
This product is specific for protein corresponding to SOAT1 Uniprot# P35610, Gene ID# 6646. [Important Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOAT1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence reported in scientific literature
  • Immunoprecipitation 2-10 ug/ml lysate
  • Western Blot 1:400
Application Notes
In Western blot a band is observed at ~46 kDa representing SOAT1 along with a non-specific band at ~30 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB400-141 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for SOAT1 Antibody

  • ACACT
  • ACACT1
  • ACAT-1
  • ACATACAT1
  • acyl-Coenzyme A: cholesterol acyltransferase
  • Acyl-coenzyme A:cholesterol acyltransferase 1
  • Cholesterol acyltransferase 1
  • EC 2.3.1.26
  • RP11-215I23.2
  • SOAT1
  • STATSOAT
  • sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
  • sterol O-acyltransferase 1

Background

SOAT1 (sterol O-acyltransferase 1), also designated ACAT1, is a homotetrameric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long-chain fatty acyl coenzyme A. The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase 2), also known as ACAT2 (acyl coenzyme A), participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the ER of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1799
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
AF2894
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
NBP2-59451
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
D6050
Species: Hu
Applications: ELISA
AF2168
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
AF1584
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
MAB4260
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-25241
Species: Hu, Mu
Applications: ICC/IF, IP, WB
MAB5696
Species: Hu, Mu
Applications: WB
NBP2-45981
Species: Hu
Applications: IHC, IHC-P
6507-IL/CF
Species: Hu
Applications: BA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB2890
Species: Hu
Applications: Flow, Simple Western, WB
AF3194
Species: Hu
Applications: ICC, Simple Western, WB
NB100-56637
Species: Hu, Mu
Applications: WB
202-IL
Species: Hu
Applications: BA
DGP00
Species: Hu
Applications: ELISA

Publications for SOAT1 Antibody (NB400-141)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IF/IHC
(1)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Hamster
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB400-141 Applications Species
Heisler DB, Johnson KA, Ma DH et al. A concerted mechanism involving ACAT and SREBPs by which oxysterols deplete accessible cholesterol to restrict microbial infection eLife 2023-01-25 [PMID: 36695568] (FLOW) FLOW
Chen D, Xia S, Zhang R Et Al. Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells Molecular cell 2021-07-12 [PMID: 34289383]
Ren M, Xu H, Xia H et al. Simultaneously targeting SOAT1 and CPT1A ameliorates hepatocellular carcinoma by disrupting lipid homeostasis Cell death discovery 2021-05-29 [PMID: 34052835] (IF/IHC, Mouse) IF/IHC Mouse
Hai Q, Ritchey B, Robinet P et al. Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant Arterioscler. Thromb. Vasc. Biol. 2017-11-02 [PMID: 29097366] (WB, Mouse) WB Mouse
Bhathena J, Martoni C, Kulamarva A et al. Oral Probiotic Microcapsule Formulation Ameliorates Non-Alcoholic Fatty Liver Disease in Bio F1B Golden Syrian Hamsters PLoS One 2013-01-01 [PMID: 23554890] (WB, Hamster) WB Hamster
Song, J, Kost, C K, Jr, Martin, D S. Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats. Am J Physiol Regul Integr Comp Physiol;290(6):R1608-15. 2006-06-01 [PMID: 16469840]
Chang, CC et al. Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies. J Biol Chem;270(49):29532-40. 1995-12-08 [PMID: 7493995] (IP, ICC/IF, WB, Human) IP, ICC/IF, WB Human

Reviews for SOAT1 Antibody (NB400-141) (0)

There are no reviews for SOAT1 Antibody (NB400-141). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOAT1 Antibody (NB400-141) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOAT1 Products

Bioinformatics Tool for SOAT1 Antibody (NB400-141)

Discover related pathways, diseases and genes to SOAT1 Antibody (NB400-141). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SOAT1 Antibody (NB400-141)

Discover more about diseases related to SOAT1 Antibody (NB400-141).
 

Pathways for SOAT1 Antibody (NB400-141)

View related products by pathway.

PTMs for SOAT1 Antibody (NB400-141)

Learn more about PTMs related to SOAT1 Antibody (NB400-141).
 

Research Areas for SOAT1 Antibody (NB400-141)

Find related products by research area.

Blogs on SOAT1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SOAT1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SOAT1
Entrez
Uniprot