HMG-CoA Reductase/HMGCR Antibody (JF0981)

Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - Analysis of HMGCR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: THP-1 cell lysate

Simple Western: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - SVG-A whole cell lysate. Antibody at 1:25 dilution. Image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, IP
• Clone: JF0981
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HMG-CoA Reductase/HMGCR Antibody (JF0981) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HMG-CoA Reductase/HMGCR aa 438-489 / 888. (SwissProt: P04035 Human; SwissProt: Q01237 Mouse; SwissProt: P51639 Rat)
• Localization: Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMGCR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:500-1:2000
• Application Notes: HMG-CoA Reductase/HMGCR antibody validated for Simple Western from a verified customer review. IP: Use at an assay dependent concentration.
  See Simple Western Antibody Database for Simple Western validation: Tested in SVG-A whole cell lysate, separated by Size, antibody dilution of 1:25

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HMG-CoA Reductase/HMGCR Antibody (JF0981)

• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• EC 1.1.1
• EC 1.1.1.34
• HMG-CoA Reductase
• HMGCR
• Hydroxymethylglutaryl-CoA Reductase
• LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMG-CoA Reductase/HMGCR Antibody (NBP2-66888)(7)

Publications using NBP2-66888

• Dolcen DN The Role of Nicotinamide N-Methyltransferase in Triple-Negative Breast Cancer Gene Expression and in vivo Metastatic Potential Thesis 2022-01-01 (WB, Human)
• Matthew Grove, Hyukmin Kim, Shuhuan Pang, Jose Paz Amaya, Guoqing Hu, Jiliang Zhou, Michel Lemay, Young-Jin Son, Klaus-Armin Nave, Timothy E Behrens TEAD1 is crucial for developmental myelination, Remak bundles, and functional regeneration of peripheral nerves eLife 2024-03-08 [PMID: 38456457]
• Thapa K, Kadiri JJ, Saukkonen K et al. Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver eLife 2023-07-25 [PMID: 37490042] (Simple Western, Human)
• Y Wang, J Wang, X Li, X Xiong, J Wang, Z Zhou, X Zhu, Y Gu, D Dominissin, L He, Y Tian, C Yi, Z Fan N1-methyladenosine�methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism Nature Communications, 2021-11-02;12(1):6314. 2021-11-02 [PMID: 34728628] (Simple Western, Human)
• Dalton GD, Oh SH, Tang L et al. Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice iScience 2021-09-24 [PMID: 34568800] (WB, Mouse)
• Kohlhaas J, Jager M. A, et al. Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Exp Cell Res 2021-01-07 [PMID: 33422461] (Simple Western, Human)
• Bharadwaj S, Devi S, Sarma P et al. Prophylactic role of Premna herbacea, a dietary leafy vegetable in managing hepatic steatosis via regulating AMPK/SREBP1/ACC/HMGCR signaling pathway Food Bioscience

Review for HMG-CoA Reductase/HMGCR Antibody (NBP2-66888) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 07/03/2018

Summary

• Application: Simple Western
• Sample Tested: SVG-A whole cell lysate
• Species: Human
• Lot: HJ1226

Bioinformatics

• Gene Symbol: HMGCR