HMG-CoA Reductase/HMGCR Antibody (JF0981)

Images

 
Western Blot: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - Analysis of HMGCR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Simple Western: HMG-CoA Reductase/HMGCR Antibody (JF0981) [NBP2-66888] - SVG-A whole cell lysate. Antibody at 1:25 dilution. Image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clone
JF0981
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HMG-CoA Reductase/HMGCR Antibody (JF0981) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human HMG-CoA Reductase/HMGCR aa 438-489 / 888. (SwissProt: P04035 Human; SwissProt: Q01237 Mouse; SwissProt: P51639 Rat)
Localization
Endoplasmic reticulum membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HMGCR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:500-1:2000
Application Notes
HMG-CoA Reductase/HMGCR antibody validated for Simple Western from a verified customer review. IP: Use at an assay dependent concentration.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-66888 in the following applications:

Publications
Read Publications using
NBP2-66888 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HMG-CoA Reductase/HMGCR Antibody (JF0981)

  • 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
  • 3-hydroxy-3-methylglutaryl-CoA reductase
  • 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
  • EC 1.1.1
  • EC 1.1.1.34
  • HMG-CoA Reductase
  • HMGCR
  • Hydroxymethylglutaryl-CoA Reductase
  • LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMG-CoA Reductase/HMGCR Antibody (NBP2-66888)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Simple Western, WB.


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Simple Western
(1)
WB
(2)
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(2)
Mouse
(1)
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Showing Publications 1 - 4 of 4.
Publications using NBP2-66888 Applications Species
Dolcen DN The Role of Nicotinamide N-Methyltransferase in Triple-Negative Breast Cancer Gene Expression and in vivo Metastatic Potential Thesis 2022-01-01 (WB, Human) WB Human
Dalton GD, Oh SH, Tang L et al. Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice iScience 2021-09-24 [PMID: 34568800] (WB, Mouse) WB Mouse
Kohlhaas J, Jager M. A, et al. Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Exp Cell Res 2021-01-07 [PMID: 33422461] (Simple Western, Human) Simple Western Human
Bharadwaj S, Devi S, Sarma P et al. Prophylactic role of Premna herbacea, a dietary leafy vegetable in managing hepatic steatosis via regulating AMPK/SREBP1/ACC/HMGCR signaling pathway Food Bioscience

Review for HMG-CoA Reductase/HMGCR Antibody (NBP2-66888) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-66888:
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Simple Western
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Simple Western HMG-CoA Reductase/HMGCR NBP2-66888
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5
reviewed by:
Verified Customer
Simple Western Human 07/03/2018
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Summary

ApplicationSimple Western
Sample TestedSVG-A whole cell lysate
SpeciesHuman
LotHJ1226

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Verified Customer
07/03/2018
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol HMGCR