| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04836-20ul | |||
NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Western Blot: CYP7A1 Antibody [NBP3-04836] - Analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CYP7A1-Antibody-Western-Blot-NBP3-04836-img0001.jpg "Western Blot: CYP7A1 Antibody [NBP3-04836] - Analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836] - Immunofluorescence analysis of HepG2 cells using CYP7A1 Rabbit pAb (A10615) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04836_rabbit-polyclonal-cyp7a1-antibody-52202519313443.jpg)
![ELISA: Mouse CYP7A1 ELISA Kit (Colorimetric) [NBP3-00427] - Samples were spiked with high concentrations of Mouse CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00427_mouse-cyp7a1-elisa-kit-colorimetric-132202416121328.jpg "ELISA: Mouse CYP7A1 ELISA Kit (Colorimetric) [NBP3-00427] - Samples were spiked with high concentrations of Mouse CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CYP7A1 ELISA Kit (Colorimetric) [NBP3-00427] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00427_mouse-cyp7a1-elisa-kit-colorimetric-43202485674.jpg)
![ELISA: Rat CYP7A1 ELISA Kit (Colorimetric) [NBP3-00460] - Samples were spiked with high concentrations of Rat CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00460_rat-cyp7a1-elisa-kit-colorimetric-13220241681498.jpg "ELISA: Rat CYP7A1 ELISA Kit (Colorimetric) [NBP3-00460] - Samples were spiked with high concentrations of Rat CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CYP7A1 ELISA Kit (Colorimetric) [NBP3-00460] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00460_rat-cyp7a1-elisa-kit-colorimetric-4320249141665.jpg)
![ELISA: Human CYP7A1 ELISA Kit (Colorimetric) [NBP3-00374] - Samples were spiked with high concentrations of Human CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00374_human-cyp7a1-elisa-kit-colorimetric-13220241626155.jpg "ELISA: Human CYP7A1 ELISA Kit (Colorimetric) [NBP3-00374] - Samples were spiked with high concentrations of Human CYP7A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CYP7A1 ELISA Kit (Colorimetric) [NBP3-00374] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00374_human-cyp7a1-elisa-kit-colorimetric-4320249141653.jpg)
![Western Blot: CYP7A1 Overexpression Lysate (Adult Normal) [NBL1-09704] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP7A1.](http://images.novusbio.com/fullsize/CYP7A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09704-img0002.jpg "Western Blot: CYP7A1 Overexpression Lysate (Adult Normal) [NBL1-09704] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP7A1.")
![Western Blot: CYP7A1 Antibody [NBP3-10795] - Western blot analysis of CYP7A1 in Human PANC1 Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/CYP7A1-Antibody-Western-Blot-NBP3-10795-img0001.jpg "Western Blot: CYP7A1 Antibody [NBP3-10795] - Western blot analysis of CYP7A1 in Human PANC1 Whole Cell lysates. Antibody dilution at 1ug/ml")
![Western Blot: CYP7A1 Antibody [NBP2-83991] - Host: Rabbit. Target Name: CYP7A1. Sample Tissue: Human NCI-H226 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CYP7A1-Antibody-Western-Blot-NBP2-83991-img0001.jpg "Western Blot: CYP7A1 Antibody [NBP2-83991] - Host: Rabbit. Target Name: CYP7A1. Sample Tissue: Human NCI-H226 Whole Cell lysates. Antibody Dilution: 1ug/ml")
