HNF-4 alpha/NR2A1 Antibody - BSA Free

Staining of human cell line CACO-2 shows localization to nucleoplasm.

Immunohistochemistry-Frozen: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Frozen 8 um rat liver tissue section fixed in formalin. HNF-4 alpha antibody at 1:100 (green). Multiplexed with a myeloperoxidase antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.

Analysis in human duodenum and tonsil tissues using NBP1-89679 antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.

Staining of human duodenum shows strong nuclear positivity in glandular cells.

Staining of human tonsil shows no positivity as expected.

Staining of human kidney shows moderate nuclear positivity in cells in tubules.

Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Analysis in human cell lines Caco-2 and PC-3 using Anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

ChIP-Exo-Seq composite graph for Anti-HNF4A (NBP1-89679) tested in HepG2 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HNF-4 alpha/NR2A1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNF4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10µg per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen Validated for IHC-Fr from a verified customer review.
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HNF-4 alpha/NR2A1 Protein (NBP1-89679PEP)

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 28827690). Rat reactivity reported in the scientific literature (PMID: 26468492).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody - BSA Free

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)(5)

Publications using NBP1-89679

• C Engelmann, D Adebayo, M Oria, F De Chiara, S Novelli, A Habtesion, N Davies, F Andreola, R Jalan Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure Sci Rep, 2020-01-15;10(1):389. 2020-01-15 [PMID: 31942020]
• Zhang W, Zhong W, Sun Q et al. Hepatic overproduction of 13- HODE due to ALOX15 upregulation contributes to alcohol-induced liver injury in mice. Sci Rep 2017-08-21 [PMID: 28827690] (WB, Mouse)
• Zhong W, Li Q, Sun Q et al. Preventing Gut Leakiness and Endotoxemia Contributes to the Protective Effect of Zinc on Alcohol-Induced Steatohepatitis in Rats. J Nutr. 2015-10-14 [PMID: 26468492] (IF/IHC, WB, Rat)
• Caroline Bonner, Julie Kerr-Conte, Valery Gmyr et al. Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion. Nature Medicine 2015-04-20 [PMID: 25894829] (ICC/IF, Human)
• Mezentsev A, Amundson SA et al. Global gene expression responses to low- or high-dose radiation in a human three-dimensional tissue model. Radiat Res 2011-06-01 [PMID: 21486161]

Review for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IHC-Fr Rat 11/19/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen Rat Liver Tissue sections
• Species: Rat
• Lot: C119316

Comments

• Comments: 8um section fixed in formalin
  HNF4a antibody diluted 1:100
  Multiplexed with myeloperoxidase antibody

Bioinformatics

• Gene Symbol: HNF4A
• Entrez:
  • 3172(Human)
• Uniprot:
  • P41235()