HNF-4 alpha/NR2A1 Antibody

Images

 
Orthogonal Strategies: Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Frozen: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Frozen 8 um rat liver tissue section fixed in formalin. HNF-4 alpha antibody at 1:100 (green). Multiplexed with a myeloperoxidase antibody (red). ...read more
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human tonsil shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are ...read more
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

HNF-4 alpha/NR2A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV
Specificity
Specificity of human HNF-4 alpha/NR2A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNF4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. HNF-4 alpha antibody validated for IHC-Fr from a verified customer review.
Control Peptide
HNF-4 alpha/NR2A1 Protein (NBP1-89679PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89679 in the following applications:

Publications
Read Publications using
NBP1-89679 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25894829). Mouse reactivity reported in the scientific literature (PMID: 28827690). Rat reactivity reported in the scientific literature (PMID: 26468492).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody

  • FLJ39654
  • hepatic nuclear factor 4 alpha
  • hepatocyte nuclear factor 4, alpha
  • hepatocyte nuclear factor 4-alpha
  • HNF4 alpha
  • HNF-4 alpha
  • HNF4A
  • HNF4a7
  • HNF-4-alpha
  • HNF4alpha10/11/12
  • HNF4HNF4a8
  • MODY
  • MODY1
  • NR2A1
  • NR2A1HNF4a9
  • NR2A21
  • Nuclear receptor subfamily 2 group A member 1
  • TCF
  • TCF14
  • TCF-14
  • TCF14HNF4alpha
  • Transcription factor 14
  • Transcription factor HNF-4
  • transcription factor-14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)(4)

Review for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-89679:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen HNF-4 alpha/NR2A1 NBP1-89679
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reviewed by:
Anonymous
IHC-Fr Rat 11/19/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen Rat Liver Tissue sections
SpeciesRat
LotC119316

Comments

Comments8um section fixed in formalin
HNF4a antibody diluted 1:100
Multiplexed with myeloperoxidase antibody

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (0)

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Recent Reviews

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5
1
4
0
3
0
2
0
1
0

Anonymous
11/19/2019
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol HNF4A
Entrez
Uniprot