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HNF-4 alpha/NR2A1 Antibody

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 (1 Review)
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Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Orthogonal Strategies: Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Frozen: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Frozen 8 um rat liver tissue section fixed in formalin. HNF-4 alpha antibody at 1:100 (green). Multiplexed with a myeloperoxidase antibody (red). ...read more
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human tonsil shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are ...read more
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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HNF-4 alpha/NR2A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV
Specificity
Specificity of human HNF-4 alpha/NR2A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNF4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. HNF-4 alpha antibody validated for IHC-Fr from a verified customer review.
Control Peptide
HNF-4 alpha/NR2A1 Protein (NBP1-89679PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89679 in the following applications:
Publications
Read Publications using
NBP1-89679 in the following applications:

  • ICC/IF
    1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25894829). Mouse reactivity reported in the scientific literature (PMID: 28827690). Rat reactivity reported in the scientific literature (PMID: 26468492).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody

  • FLJ39654
  • hepatic nuclear factor 4 alpha
  • hepatocyte nuclear factor 4, alpha
  • hepatocyte nuclear factor 4-alpha
  • HNF4 alpha
  • HNF-4 alpha
  • HNF4A
  • HNF4a7
  • HNF-4-alpha
  • HNF4alpha10/11/12
  • HNF4HNF4a8
  • MODY
  • MODY1
  • NR2A1
  • NR2A1HNF4a9
  • NR2A21
  • Nuclear receptor subfamily 2 group A member 1
  • TCF
  • TCF14
  • TCF-14
  • TCF14HNF4alpha
  • Transcription factor 14
  • Transcription factor HNF-4
  • transcription factor-14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Analysis in human kidney tissue.
Immunocytochemistry/Immunofluorescence: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Nuclear expression of HNF1B (green) in a subset of PDX1 expressing cells (blue) in human pancreas. Image submitted by a verified customer review.
(1 Review)
(6 Publications)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
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Western Blot: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of TCF7L2 expression in K-562 (Cat # L009V1).
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Immunohistochemistry-Paraffin: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
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Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
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Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] diluted at 1:500.Blue: Hoechst 33342 staining.
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Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).
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(1 Review)
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Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.
(3 Publications)
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Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.
(4 Reviews)
(31 Publications)
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[Unconjugated]
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TCF7/TCF1 at approximately 60, 40, and 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TCF7/TCF1 was detected in immersion fixed HCT‑116 human colorectal carcinoma cell line using Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TCF7/TCF1 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC, ICC
PXR/NR1I2 Antibody
(1D12G1)
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Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)
Immunocytochemistry/Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of Hela cells using NR1I2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 Secondary antibody was used.
Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against HEK293 (1) and NR1I2 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Coagulation Factor
XIV/Protein C Antibod ...
NBP2-66987
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
HNF-4 alpha/NR2A1
Antibody
NBP1-89679
HNF-4 alpha/NR2A1 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


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(1)
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(2)
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Review for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-89679:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen HNF-4 alpha/NR2A1 NBP1-89679
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reviewed by:
Anonymous
 IHC-Fr Rat 11/19/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen Rat Liver Tissue sections
SpeciesRat
LotC119316

Comments

Comments8um section fixed in formalin
HNF4a antibody diluted 1:100
Multiplexed with myeloperoxidase antibody

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Immunohistochemistry-Frozen HNF-4 alpha/NR2A1 NBP1-89679
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 Anonymous
11/19/2019
Application: IHC-Fr
Species: Rat
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Bioinformatics

Gene Symbol HNF4A
Entrez
Uniprot