Home » HNF-4 alpha/NR2A1 » HNF-4 alpha/NR2A1 Antibodies » HNF-4 alpha/NR2A1 Antibody

HNF-4 alpha/NR2A1 Antibody

 (2 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell line HepG2.
Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell line HepG2.
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human small intestine shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

HNF-4 alpha/NR2A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (91%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNF4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended.
Control Peptide
HNF-4 alpha/NR2A1 Protein (NBP1-89679PEP)
Publications
Read Publications using
NBP1-89679 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25894829).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody

  • FLJ39654
  • hepatic nuclear factor 4 alpha
  • hepatocyte nuclear factor 4, alpha
  • hepatocyte nuclear factor 4-alpha
  • HNF4 alpha
  • HNF-4 alpha
  • HNF4A
  • HNF4a7
  • HNF-4-alpha
  • HNF4alpha10/11/12
  • HNF4HNF4a8
  • MODY
  • MODY1
  • NR2A1
  • NR2A1HNF4a9
  • NR2A21
  • Nuclear receptor subfamily 2 group A member 1
  • TCF
  • TCF14
  • TCF-14
  • TCF14HNF4alpha
  • Transcription factor 14
  • Transcription factor HNF-4
  • transcription factor-14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HNF1 Antibody
NBP1-33596
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
beta-Catenin
Antibody (12F7)
NBP1-54467
Western Blot (Negative): beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
(3 Reviews)
(6 Publications)
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
TCF-2/HNF-1 beta
Antibody
NBP1-89680
Western Blot: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Analysis in human kidney tissue.
Immunocytochemistry/Immunofluorescence: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Immunohistochemical staining of human kidney shows moderate to strong nuclear positivity in renal tubules.
(1 Review)
(4 Publications)
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
TCF7L2 Antibody
(3A4)
H00006934-M06
Western Blot: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of TCF7L2 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
LEF1 Antibody
NBP1-86960
Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell line MOLT-4.
Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Glucokinase/GCK
Antibody (3E3)
NBP1-47778
Western Blot: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.
Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Human prostate tissue.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(3 Reviews)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
beta-Catenin
Antibody (12F7)
NBP1-54467
Western Blot (Negative): beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
(3 Reviews)
(6 Publications)
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
MLX Antibody
AF4186
Western blot shows nuclear extracts of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
(1 Publication)
Species: Hu
Applications: WB, ICC
APC Antibody
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.
Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TCF4 Antibody (1G4)
H00006925-M03
Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: TCF4 Antibody (1G4) [H00006925-M03] - Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.
(1 Review)
(3 Publications)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF
Cyclin D1 Antibody
NBP2-24695
Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.
(2 Publications)
Species: Hu
Applications: WB, IHC-P
c-Myc Antibody
NB600-335
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
(1 Review)
(34 Publications)
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
Axin-1 Antibody
AF3287
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB
TCF7/TCF1 Antibody
[Unconjugated]
AF5596
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TCF7/TCF1 at approximately 60, 40, and 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TCF7/TCF1 was detected in immersion fixed HCT‑116 human colorectal carcinoma cell line using Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TCF7/TCF1 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC, ICC
PXR/NR1I2 Antibody
(H4417) [Unconjugated ...
PP-H4417-00
PXR/NR1I2 Antibody (H4417) [Unconjugated]
(1 Publication)
Species: Hu
Applications: WB, IP, DirELISA
Coagulation Factor
XIV/Protein C Antibod ...
AF4885
Coagulation Factor XIV/Protein C Antibody
(1 Publication)
Species: Mu
Applications: WB, IP
HNF-4 alpha/NR2A1
Antibody
NBP1-89679
HNF-4 alpha/NR2A1 Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (0)

There are no reviews for HNF-4 alpha/NR2A1 Antibody (NBP1-89679). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HNF-4 alpha/NR2A1 Antibody (NBP1-89679) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HNF-4 alpha/NR2A1 Antibody Products

Related Products by Gene

APC, AXIN1, BETA-CATENIN, CATNB, CCND1, CTNNB1, GCK, HEPATOCYTE NUCLEAR FACTOR 1, HNF1A, HNF1B, INS, LEF1, MLX, MYC, NR1I2, PROC, TCF4, TCF7, TCF7L2, WNT

Bioinformatics Tool for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)

Discover related pathways, diseases and genes to HNF-4 alpha/NR2A1 Antibody (NBP1-89679). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)

Discover more about diseases related to HNF-4 alpha/NR2A1 Antibody (NBP1-89679).
 

Pathways for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)

View related products by pathway.

PTMs for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)

Learn more about PTMs related to HNF-4 alpha/NR2A1 Antibody (NBP1-89679).
 

Research Areas for HNF-4 alpha/NR2A1 Antibody (NBP1-89679)

Find related products by research area.

Blogs on HNF-4 alpha/NR2A1

There are no specific blogs for HNF-4 alpha/NR2A1, but you can read our latest blog posts.

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HNF-4 alpha/NR2A1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HNF4A
Entrez
Uniprot

Customers Who Bought This Also Bought