Carboxylesterase 1/CES1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.

Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human lung shows strong cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.

Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Simple Western lane view shows a specific band for CES1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Electropherogram image(s) of corresponding Simple Western lane view. CES1 antibody was used at 1:20 dilution on RT-4 lysate(s).

Staining of human cerebral cortex, liver, prostate and upper gastrointestinal using NBP1-85691 (A) shows similar protein distribution across tissues to independent antibody NBP2-48854 (B).

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Analysis in human cell line HepG2.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Carboxylesterase 1/CES1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Carboxylesterase 1/CES1 Antibody - BSA Free (NBP1-85691) is a polyclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CES1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control Peptide:

  Carboxylesterase 1/CES1 Recombinant Protein Antigen (NBP1-85691PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Carboxylesterase 1/CES1 Antibody - BSA Free

• ACAT
• acyl coenzyme A:cholesterol acyltransferase
• Acyl-coenzyme A:cholesterol acyltransferase
• Brain carboxylesterase hBr1
• carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Carboxylesterase 1
• carboxylesterase 2 (liver)
• CEH
• CES1
• Ces-1
• CES1A2
• CES2EC 3.1.1.1
• cholesteryl ester hydrolase
• Cocaine carboxylesterase
• EC 3.1.1
• Egasyn
• HMSE1
• HMSECES1A1
• human monocyte/macrophage serine esterase 1
• liver carboxylesterase 1
• MGC117365
• Monocyte/macrophage serine esterase
• PCE-1
• REH
• Retinyl ester hydrolase
• Serine esterase 1
• SES1
• Ses-1
• SES1TGH
• TGH
• Triacylglycerol hydrolase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CES1