ACAT1 Antibody

Images

 
Genetic Strategies: Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.
Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.
Independent Antibodies: Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ACAT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMV
Specificity
Specificity of human ACAT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. Western Blot verified by a customer review.
Control Peptide
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89284 in the following applications:

Publications
Read Publications using NBP1-89284.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (80%), Rat (81%). Reactivity reported in scientific literature (PMID: 22361696). Porcine reactivity per customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACAT1 Antibody

  • ACAT
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACAT1 Antibody (NBP1-89284)(5)

Review for ACAT1 Antibody (NBP1-89284) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using NBP1-89284:
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WB
(1)
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Pig
(1)
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Images Ratings Applications Species Date Details
Western Blot ACAT1 NBP1-89284
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reviewed by:
Jennifer McGuire
WB Pig 07/24/2018
View

Summary

ApplicationWestern Blot
Sample Testedbrain cortical homogenate,Brain (cerebral cortex)
SpeciesPig
LotA113800

Comments

Commentsprimary concentration 1:500, Secondary concentration 1:10,000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACAT1 Antibody (NBP1-89284) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ACAT1 Products

Array NBP1-89284

Bioinformatics Tool for ACAT1 Antibody (NBP1-89284)

Discover related pathways, diseases and genes to ACAT1 Antibody (NBP1-89284). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ACAT1 Antibody (NBP1-89284)

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Pathways for ACAT1 Antibody (NBP1-89284)

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PTMs for ACAT1 Antibody (NBP1-89284)

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Research Areas for ACAT1 Antibody (NBP1-89284)

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Blogs on ACAT1

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Calculators

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Recent Reviews

5
5
1
4
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2
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1
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Jennifer McGuire
07/24/2018
Application: WB
Species: Pig

Bioinformatics

Gene Symbol ACAT1