CYBB/NOX2 Antibody (NL7) - BSA Free

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.

Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: NL7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CYBB/NOX2 Antibody (NL7) - BSA Free Summary

• Immunogen: Cytochrome B245 solubilised in octyl-beta-glucopyranoside
• Epitope: Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
• Specificity: This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYBB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CYBB/NOX2 Antibody (NL7) - BSA Free

• CGD
• CGD91-phox
• CYBB
• CYBB/NOX2
• Cytochrome b(558) subunit beta
• cytochrome b-245 heavy chain
• cytochrome b-245, beta polypeptide
• Cytochrome b558 subunit beta
• EC 1.-.-.-
• EC 1.6.3
• gp91-1
• GP91PHOX
• gp91-phox
• Heme-binding membrane glycoprotein gp91phox
• NADPH oxidase 2
• Neutrophil cytochrome b 91 kDa polypeptide
• NOX2
• NOX2chronic granulomatous disease
• p22 phagocyte B-cytochrome
• p91-PHOX
• Superoxide-generating NADPH oxidase heavy chain subunit

Background

Cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYBB/NOX2 Antibody (NBP1-41012)(3)

Publications using NBP1-41012

• Park Y, Park M, Kim J et al. NOX2-Induced High Glycolytic Activity Contributes to the Gain of COL5A1-Mediated Mesenchymal Phenotype in GBM Cancers 2022-01-20 [PMID: 35158782] (IHC-P, Human)
• Wu Zhi-Jie, Zhao Xin, Banaszak Lauren G et al. CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation. Int J Oncol. 2018-01-01 [PMID: 29532865] (FLOW, Human)
• Burrit, J B et al. Functional epitope on human neutrophil flavocytochrome b558. J Immunol 170: 6082 - 6089. 2003-01-01 [PMID: 12794137] (FLOW)

Reviews for CYBB/NOX2 Antibody (NBP1-41012) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Chicken and Mouse.

reviewed by: Verified Customer WB Chicken and Mouse 12/06/2016

Summary

• Application: Western Blot
• Sample Tested: Chicken cell lines,Mouse macrophages
• Species: Chicken and Mouse
• Lot: 0309

Comments

• Comments: NSCS16
  
  I do have a full experimental report that can be emailed.
  It works for mouse, but not chicken. We were hoping it would work for chicken.

reviewed by: Tariq Syed IHC-P Human 04/18/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human placental membrane intact
• Species: Human
• Lot: 0309

FAQs for CYBB/NOX2 Antibody (NBP1-41012). (Showing 1 - 1 of 1 FAQs).

1. I have a question about an antibody we have purchased and are using currently- NBP1-41012- CYBB/NOX2. My question is in regards to the packaging of this antibody. Is there a portion of BSA or is this antibody carrier free? I would like to proceed with con
   • I have taken a look at the buffer components of NBP1-41012 can confirm that there is no BSA in the buffer. This anitbody is carrier free and can be conjugated. The buffer contains PBS and 0.09% sodium azide.

Bioinformatics

• Gene Symbol: CYBB
• Entrez:
  • 1536(Human)
• Uniprot:
  • P04839(Human)