CYBB/NOX2 Antibody (NL7) - BSA Free

Images

 
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched ...read more
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CYBB/NOX2 Antibody (NL7) - BSA Free Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-41012 in the following applications:

Publications
Read Publications using
NBP1-41012 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CYBB/NOX2 Antibody (NL7) - BSA Free

  • CGD
  • CGD91-phox
  • CYBB
  • CYBB/NOX2
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.-.-.-
  • EC 1.6.3
  • gp91-1
  • GP91PHOX
  • gp91-phox
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Background

Cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYBB/NOX2 Antibody (NBP1-41012)(3)

Reviews for CYBB/NOX2 Antibody (NBP1-41012) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Chicken and Mouse.

Reviews using NBP1-41012:
Filter by Applications
Western Blot
(1)
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Human
(1)
Chicken and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CYBB/NOX2 NBP1-41012
Enlarge
4
reviewed by:
Verified Customer
Western Blot Chicken and Mouse 12/06/2016
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines,Mouse macrophages
SpeciesChicken and Mouse
Lot0309

Comments

CommentsNSCS16

I do have a full experimental report that can be emailed.
It works for mouse, but not chicken. We were hoping it would work for chicken.

Immunohistochemistry-Paraffin CYBB/NOX2 NBP1-41012
Enlarge
5
reviewed by:
Tariq Syed
Immunohistochemistry-Paraffin Human 04/18/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman placental membrane intact
SpeciesHuman
Lot0309

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYBB/NOX2 Antibody (NBP1-41012). (Showing 1 - 1 of 1 FAQs).

  1. I have a question about an antibody we have purchased and are using currently- NBP1-41012- CYBB/NOX2. My question is in regards to the packaging of this antibody. Is there a portion of BSA or is this antibody carrier free? I would like to proceed with con
    • I have taken a look at the buffer components of NBP1-41012 can confirm that there is no BSA in the buffer. This anitbody is carrier free and can be conjugated. The buffer contains PBS and 0.09% sodium azide.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CYBB/NOX2 Antibody (NBP1-41012)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
12/06/2016
Application: Western Blot
Species: Chicken and Mouse

Tariq Syed
04/18/2013
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol CYBB
Entrez
Uniprot