CYBB/NOX2 Antibody (NL7)

Images

 
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched ...read more
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CYBB/NOX2 Antibody (NL7) Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-41012 in the following applications:

Publications
Read Publications using
NBP1-41012 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CYBB/NOX2 Antibody (NL7)

  • CGD
  • CGD91-phox
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.6.3
  • GP91-1
  • GP91PHOX
  • GP91-PHOX
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Background

Cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYBB/NOX2 Antibody (NBP1-41012)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, Flow.


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FLOW
(1)
Flow
(1)
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Filter By Species
Human
(1)
All Species

Reviews for CYBB/NOX2 Antibody (NBP1-41012) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Chicken and Mouse.

Reviews using NBP1-41012:
Filter by Applications
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Chicken and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CYBB/NOX2 NBP1-41012
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reviewed by:
James Petitte
WB Chicken and Mouse 12/06/2016
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines,Mouse macrophages
SpeciesChicken and Mouse
Lot0309

Comments

CommentsNSCS16

I do have a full experimental report that can be emailed.
It works for mouse, but not chicken. We were hoping it would work for chicken.

Immunohistochemistry-Paraffin CYBB/NOX2 NBP1-41012
Enlarge
reviewed by:
Anonymous
IHC-P Human 04/18/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman placental membrane intact
SpeciesHuman
Lot0309
CommentsPrior to immunohistochemistry, some sections were treated with antigen retrieval to facilitate antibody binding to antigen. Briefly, slides were incubated for 20 minutes in Target Retrieval Solution (Dako Corporation, Carpinteria, CA. Cat. #S1699) preheated to 99*C. They were then taken out of the steamer and placed on an open counter to let cool down for 20 minutes in the solution. The slides were then rinsed in three changes of distilled water and placed into a container of Tris Buffered Saline with tween 20 for five minutes to decrease the surface tension of the slides, and facilitate coating by the following reagents.

Blocking

Blocking DetailsBlocking with Avidin/Biotin each for 15 min then Casein solution for 30 min

Primary Anitbody

Dilution Ratio1:200 at room temperature for 1 hour

Secondary Antibody

Secondary DescriptionLSAB2 Streptavidin-HRP
Secondary Manufacturer Cat#Dako cat #K0675
Secondary Concentration1:200

Details

Detection NotesDako - LSAB 2 Streptavidin-HRP Ready-to-use cat #K1016
Fixation DetailsFormalin fixed - Paraffin embedded intact placental tissue
Wash DescriptionTris Buffered Saline with tween 20

Comments

CommentsPrior to immunohistochemistry, some sections were treated with antigen retrieval to facilitate antibody binding to antigen. Briefly, slides were incubated for 20 minutes in Target Retrieval Solution (Dako Corporation, Carpinteria, CA. Cat. #S1699) preheated to 99*C. They were then taken out of the steamer and placed on an open counter to let cool down for 20 minutes in the solution. The slides were then rinsed in three changes of distilled water and placed into a container of Tris Buffered Saline with tween 20 for five minutes to decrease the surface tension of the slides, and facilitate coating by the following reagents.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYBB/NOX2 Antibody (NBP1-41012). (Showing 1 - 1 of 1 FAQs).

  1. I have a question about an antibody we have purchased and are using currently- NBP1-41012- CYBB/NOX2. My question is in regards to the packaging of this antibody. Is there a portion of BSA or is this antibody carrier free? I would like to proceed with con
    • I have taken a look at the buffer components of NBP1-41012 can confirm that there is no BSA in the buffer. This anitbody is carrier free and can be conjugated. The buffer contains PBS and 0.09% sodium azide.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-41012AF350
Alexa Fluor 405 NBP1-41012AF405
Alexa Fluor 488 NBP1-41012AF488
Alexa Fluor 532 NBP1-41012AF532
Alexa Fluor 594 NBP1-41012AF594
Alexa Fluor 647 NBP1-41012AF647
Alexa Fluor 700 NBP1-41012AF700
Alexa Fluor 750 NBP1-41012AF750
Allophycocyanin NBP1-41012APC
Allophycocyanin/Cy7 NBP1-41012APCCY7
DyLight 350 NBP1-41012UV
DyLight 405 NBP1-41012V
DyLight 488 NBP1-41012G
DyLight 550 NBP1-41012R
DyLight 594 NBP1-41012DL594
DyLight 650 NBP1-41012C
DyLight 680 NBP1-41012FR
DyLight 755 NBP1-41012IR
Janelia Fluor 549 NBP1-41012JF549
Janelia Fluor 646 NBP1-41012JF646
PE NBP1-41012PE
PE/Atto594 NBP1-41012PEATT594
PE/Cy5.5 NBP1-41012PECY55
PE/Cy7 NBP1-41012PECY7
PerCP NBP1-41012PCP

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Bioinformatics Tool for CYBB/NOX2 Antibody (NBP1-41012)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

James Petitte
12/06/2016
Application: WB
Species: Chicken and Mouse

Anonymous
04/18/2013
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CYBB
Entrez
Uniprot