NOX1 Antibody

Images

 
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - Nox1 antibody detects Nox1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 ...read more
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Human lung carcinoma. NOX1 antibody diluted at 1:500.
Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG ...read more
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOX1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
Localization
Membrane, Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Application Notes
IP usage reported in scientific literature (PMID: 27094494). NOX1 antibody validated for FLOW from a verified customer review.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31546 in the following applications:

Publications
Read Publications using
NBP1-31546 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    9 publications

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NOX1 Antibody

  • EC 1.6.3
  • GP91-2
  • Mitogenic oxidase 1
  • MOX-1
  • MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
  • NADPH oxidase 1
  • NADPH oxidase homolog-1
  • NOH1
  • NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
  • NOH-1NADPH oxidase 1 variant NOH-1L
  • NOX1
  • NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-41291
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB110-58849
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb, Sh
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-790
Species: Hu, Mu, Po
Applications: WB, Flow, IHC, IHC-P, PEP-ELISA, IF
NBP1-59573
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Rb, Sh
Applications: WB, IHC
NB300-950
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-92197
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB1904
Species: Hu
Applications: WB, IHC, ICC
NBP1-89163
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-1343
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-13677
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF5758
Species: Hu
Applications: WB, IHC, ICC
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-52105
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
NBP1-33736
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84254
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for NOX1 Antibody (NBP1-31546)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(9)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP1-31546 Applications Species
Yoshimura Y Resveratrol slows cell growth by targeting the Warburg effect and stimulating mitochondria metabolism Thesis 2019 Jun 28 [PMID: 31304204] (WB, Human) WB Human
Diebold BA, Wilder SG, De Deken X et al. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR Methods Mol. Biol. 2019 Jun 08 [PMID: 31172474]
Kaushik DK, Bhattacharya A, Mirzaei R et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance J. Clin. Invest. May 13 2019 [PMID: 31081803] (WB, Human) WB Human
Vara D, Cifuentes-Pagano E, Pagano PJ, Pula G. A novel combinatorial technique for simultaneous quantification of oxygen radicals and aggregation reveals unexpected redox patterns in the activation of platelets by different physiopathological stimuli Haematologica 2019 Jan 24 [PMID: 30679320] (IP, Human, Mouse) IP Human, Mouse
Raut PK, Kim SH, Choi DY et al. Growth of breast cancer cells by leptin is mediated via activation of the inflammasome: Critical roles of estrogen receptor signaling and reactive oxygen species production. Biochem. Pharmacol. Jan 10 2019 [PMID: 30633869] (WB, Human) WB Human
Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. Sep 30 2018 [PMID: 30363917] (WB, Human, Mouse) WB Human, Mouse
Shin SK, Cho JH, Kim EJ et al. Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model World J. Gastroenterol. 2017 Jul 07 [PMID: 28740344] (ICC/IF, Mouse) ICC/IF Mouse
Kangawa Y, Yoshida T, Maruyama K et al. Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation. Food Chem. Toxicol. Dec 15 2016 [PMID: 27989839] (Mouse) Mouse
Wyssenbach A, Quintela T, Llavero F et al. Amyloid B-induced astrogliosis is mediated by B1-integrin via NADPH oxidase 2 in Alzheimer's disease. Aging Cell Oct 5 2016 [PMID: 27709751] (IHC-P, Mouse) IHC-P Mouse
Pekarcikova L, Knopfova L, Benes P, Smarda J c-Myb regulates NOX1/p38 to control survival of colorectal carcinoma cells. Cell Signal 2016 Aug [PMID: 27107996]
Show All 17 Publications.

Review for NOX1 Antibody (NBP1-31546) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31546:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry NOX1 NBP1-31546
Enlarge
reviewed by:
Renato Gaspar
Flow Human 01/24/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedPlatelets
SpeciesHuman
Lot42214

Comments

CommentsPlatelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOX1 Antibody (NBP1-31546) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NOX1 Products

Bioinformatics Tool for NOX1 Antibody (NBP1-31546)

Discover related pathways, diseases and genes to NOX1 Antibody (NBP1-31546). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NOX1 Antibody (NBP1-31546)

Discover more about diseases related to NOX1 Antibody (NBP1-31546).
 

Pathways for NOX1 Antibody (NBP1-31546)

View related products by pathway.

PTMs for NOX1 Antibody (NBP1-31546)

Learn more about PTMs related to NOX1 Antibody (NBP1-31546).

Blogs on NOX1.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Renato Gaspar
01/24/2019
Application: Flow
Species: Human

Bioinformatics

Gene Symbol NOX1
Entrez
Uniprot