NOX1 Antibody - Azide Free

Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. pSample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: NOX1 Antibody [NBP1-31546] - Blueberry polyphenol extract (BPE) did not affect the expression of NADPH oxidases (NOX) in angiotensin (Ang) II-treated human aortic endothelial cells (HAECs). HAECs were treated with 200 µg/mL of BPE for 1 h then treated with 200 nM of Ang II for 12 h. Protein expression of NOX1 (A,B), NOX2 (C,D), NOX4 (E,F), & NOX5 (G,H) were determined by Western blot. Quantification was performed using Image Lab (Bio-Rad Laboratories, Inc.). Data are expressed as mean ± SD from three (NOX1 & NOX4) & five (NOX2 & NOX5) independent experiments. Values that do not share the same letter are significantly different from each other (p ≤ 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35453301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NOX1 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit NOX1 Antibody - Azide Free (NBP1-31546) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-NOX1 Antibody: Cited in 34 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
• Localization: Membrane, Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Validated from a verified customer review.
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Reported in scientific literature (PMID: 27094494)
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for NOX1 Antibody - Azide Free

• EC 1.6.3
• GP91-2
• Mitogenic oxidase 1
• MOX-1
• MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
• NADPH oxidase 1
• NADPH oxidase homolog-1
• NOH1
• NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
• NOH-1NADPH oxidase 1 variant NOH-1L
• NOX1
• NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOX1 Antibody (NBP1-31546)(35)

Publications using NBP1-31546

• Scharmacher J, Wartenberg M, Sauer H The pro-inflammatory signature of lipopolysaccharide in spontaneous contracting embryoid bodies differentiated from mouse embryonic stem cells Journal of cellular and molecular medicine 2023-06-14 [PMID: 37315183] (WB, Mouse)
• Yao G, Li H, Zuo X et al. Oscillatory shear stress promotes vein graft intimal hyperplasia via NADPH oxidase-related pathways Frontiers in Surgery 2023-02-13 [PMID: 36860953]
• Hwang S, Kim SH, Yoo KH et al. Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes BMC molecular and cell biology 2022-12-14 [PMID: 36517746]
• Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Mouse, Human)
• Sevilla-Montero J, Munar-Rubert O, Pino-Fadón J et Al. Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase Free Radic Biol Med 2022-12-06 [PMID: 36174878] (Western Blot)
• Najjar R, Mu S, Feresin R Blueberry Polyphenols Increase Nitric Oxide and Attenuate Angiotensin II-Induced Oxidative Stress and Inflammatory Signaling in Human Aortic Endothelial Cells Antioxidants 2022-03-23 [PMID: 35453301] (WB, Human)
• Tarafdar A, Wolska N, Krisp C et al. The amyloid peptide beta disrupts intercellular junctions and increases endothelial permeability in a NADPH oxidase 1-dependent manner Redox Biology 2022-06-01 [PMID: 35358850] (WB, Human)
• Oh S, Yang JY, Park CH Et al. Dieckol Reduces Muscle Atrophy by Modulating Angiotensin Type II Type 1 Receptor and NADPH Oxidase in Spontaneously Hypertensive Rats Antioxidants (Basel, Switzerland) 2021-09-30 [PMID: 34679696] (WB, Rat)
• Gaspar R, Sage T, Little G et al. Protein Disulphide Isomerase and NADPH Oxidase 1 Cooperate to Control Platelet Function and Are Associated with Cardiometabolic Disease Risk Factors Antioxidants 2021-03-23 [PMID: 33806982] (ICC/IF, Human)
• Gaspar RS, Ferreira PM, Mitchell JL et al. Platelet-derived extracellular vesicles express NADPH oxidase-1 (Nox-1), generate superoxide and modulate platelet function Free Radical Biology and Medicine 2021-03-01 [PMID: 33548451]
• Pankaj Attri, Jae-Hyun Park, Joey De Backer, Myeongkyu Kim, Ji-Hye Yun, Yunseok Heo, Sylvia Dewilde, Masaharu Shiratani, Eun Ha Choi, Weontae Lee, Annemie Bogaerts Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study. International journal of biological macromolecules 2021-04-01 [PMID: 32961197]
• Hong G, Davies C, Omole Z et al. Campylobacter jejuni modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells bioRxiv 2022-03-07 (WB, Human)
• Kumar Raut P, Park P Globular adiponectin antagonizes leptin-induced growth of cancer cells by modulating inflammasomes activation: Critical role of HO-1 signaling Biochemical Pharmacology 2020-07-01 [PMID: 32745467]
• Wang X, Zhang S, Ding Y et al. p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis Redox Biol 2020-05-11 [PMID: 32422541] (WB, Mouse)
• Wang C, Zhu L, Yuan W et al. Diabetes aggravates myocardial ischaemia reperfusion injury via activating Nox2-related programmed cell death in an AMPK-dependent manner J. Cell. Mol. Med. 2020-04-29 [PMID: 32351005] (WB, Rat)
• Cao L, Li X, Xu R et al. DUOX2, a common modulator in preventive effects of monoamine-based antidepressants on water immersion restraint stress- and indomethacin- induced gastric mucosal damage European Journal of Pharmacology 2020-03-01 [PMID: 32131022] (WB, Rat)
• Shin SK, Kim KO, Kim SH et al. Exogenous 8-hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1-NADPH oxidase signaling J. Gastroenterol. Hepatol. 2020-01-06 [PMID: 31907970] (IHC-P WB, Human, Rat)
• Yoshimura Y Resveratrol slows cell growth by targeting the Warburg effect and stimulating mitochondria metabolism Thesis 2019-06-28 [PMID: 31304204] (WB, Human)
• Diebold BA, Wilder SG, De Deken X et al. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR Methods Mol. Biol. 2019-06-08 [PMID: 31172474]
• Kaushik DK, Bhattacharya A, Mirzaei R et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance J. Clin. Invest. 2019-05-13 [PMID: 31081803] (WB, Human)
• Vara D, Cifuentes-Pagano E, Pagano PJ, Pula G. A novel combinatorial technique for simultaneous quantification of oxygen radicals and aggregation reveals unexpected redox patterns in the activation of platelets by different physiopathological stimuli Haematologica 2019-01-24 [PMID: 30679320] (IP, Mouse, Human)
• Raut PK, Kim SH, Choi DY et al. Growth of breast cancer cells by leptin is mediated via activation of the inflammasome: Critical roles of estrogen receptor signaling and reactive oxygen species production. Biochem. Pharmacol. 2019-01-10 [PMID: 30633869] (WB, Human)
• Tian HP, Sun YH, He L et al. Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II. Int Heart J. 2018-10-25 [PMID: 30369577]
• Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. 2018-09-30 [PMID: 30363917] (WB, Mouse, Human)
• Shin SK, Cho JH, Kim EJ et al. Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model World J. Gastroenterol. 2017-07-07 [PMID: 28740344] (ICC/IF, Mouse)
• Kangawa Y, Yoshida T, Maruyama K et al. Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation. Food Chem. Toxicol. 2016-12-15 [PMID: 27989839] (Mouse)
• Wyssenbach A, Quintela T, Llavero F et al. Amyloid B-induced astrogliosis is mediated by B1-integrin via NADPH oxidase 2 in Alzheimer's disease. Aging Cell 2016-10-05 [PMID: 27709751] (IHC-P, Mouse)
• Pekarcikova L, Knopfova L, Benes P, Smarda J c-Myb regulates NOX1/p38 to control survival of colorectal carcinoma cells. Cell Signal 2016-08-01 [PMID: 27107996]
• Kwon J, Wang A, Burke DJ et al. Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free Radic. Biol. Med. 2016-04-14 [PMID: 27094494] (IP, WB, Human)
• Oxidase N, Fibrosis IC, Pathways S. NADPH Oxidase 4 Induces Cardiac Fibrosis andHypertrophy Through Activating Akt/mTOR and NFkBSignaling Pathways Circulation. 2014-12-12 [PMID: 25589557] (WB, Mouse)
• Wu CH, Tang SC, Wang PH et al. Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation. J Biol Chem 2012-07-01 [PMID: 22648416] (WB, Human)
• Dvoriantchikova G, Grant J, Santos AR et al. Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia. Invest Ophthalmol Vis Sci 2012-05-01 [PMID: 22467573] (WB, Human)
• Musset B, Clark RA, DeCoursey TE et al. NOX5 in human spermatozoa: expression, function, and regulation. J Biol Chem 2012-03-01 [PMID: 22291013] (WB, Human)
• Cao JY, Mansouri S, Frappier L. Changes in the Nasopharyngeal Carcinoma Nuclear Proteome Induced by the EBNA1 Protein of Epstein-Barr Virus Reveal Potential Roles for EBNA1 in Metastasis and Oxidative Stress Responses. J Virol. 2011-10-19 [PMID: 22013061]
• Balasubramaniyan V, Wright G, Sharma V et al. Ammonia reduction with Ornithine phenylacetate restores brain eNOS activity via the DDAH-ADMA pathway in bile duct ligated cirrhotic rats. American Journal of Physiology, Gastrointestinal and Liver Physiology. 2011-09-08 [PMID: 21903766]

Review for NOX1 Antibody (NBP1-31546) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Renato Gaspar Flow Human 01/24/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Platelets
• Species: Human
• Lot: 42214

Comments

• Comments: Platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.

Bioinformatics

• Gene Symbol: NOX1
• Entrez:
  • 27305(Human)
• Uniprot:
  • Q9Y5S8(Human)