Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by ...read more
Western Blot: NOX1 Antibody [NBP1-31546] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody diluted at 1:2000. The ...read more
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Mouse kidney. NOX1 stained by NOX1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG ...read more
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.
Western Blot: NOX1 Antibody [NBP1-31546] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - Nox1 antibody detects Nox1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 ...read more
IP usage reported in scientific literature (PMID: 27094494). NOX1 antibody validated for FLOW from a verified customer review.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-31546 in the following applications:
The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.
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