NOX1 Antibody

Images

 
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - Nox1 antibody detects Nox1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 ...read more
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Human lung carcinoma. NOX1 antibody diluted at 1:500.
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOX1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
Localization
Membrane, Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Application Notes
IP usage reported in scientific literature (PMID: 27094494).
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-31546 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NOX1 Antibody

  • EC 1.6.3
  • GP91-2
  • Mitogenic oxidase 1
  • MOX-1
  • MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
  • NADPH oxidase 1
  • NADPH oxidase homolog-1
  • NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
  • NOH-1NADPH oxidase 1 variant NOH-1L
  • NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-31546
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for NOX1 Antibody (NBP1-31546)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-31546 Applications Species
Shin SK, Cho JH, Kim EJ et al. Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model World J. Gastroenterol. 2017 Jul 07 [PMID: 28740344] (ICC/IF, Mouse) ICC/IF Mouse
Kangawa Y, Yoshida T, Maruyama K et al. Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation. Food Chem. Toxicol. Dec 15 2016 [PMID: 27989839] (Mouse) Mouse
Wyssenbach A, Quintela T, Llavero F et al. Amyloid B-induced astrogliosis is mediated by B1-integrin via NADPH oxidase 2 in Alzheimer's disease. Aging Cell Oct 5 2016 [PMID: 27709751] (IHC-P, Mouse) IHC-P Mouse
Pekarcikova L, Knopfova L, Benes P, Smarda J c-Myb regulates NOX1/p38 to control survival of colorectal carcinoma cells. Cell Signal 2016 Aug [PMID: 27107996]
Kwon J, Wang A, Burke DJ et al. Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free Radic. Biol. Med. 2016 Apr 14 [PMID: 27094494] (WB, IP, Human) WB, IP Human
Oxidase N, Fibrosis IC, Pathways S. NADPH Oxidase 4 Induces Cardiac Fibrosis andHypertrophy Through Activating Akt/mTOR and NFkBSignaling Pathways Circulation. 2014 Dec 12 [PMID: 25589557] (WB, Mouse)

Details:
NOX1 antibody was used for WB on lysates of left heart ventricle/LV tissues from Lox-Stop-lox-human Nox4 Tg mice (LSL-hNox4Tg) on a mixed genetic background of C57BL/6 (Figure 2B)
WB Mouse
Wu CH, Tang SC, Wang PH et al. Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation. J Biol Chem 2012 Jul [PMID: 22648416] (WB, Human) WB Human
Dvoriantchikova G, Grant J, Santos AR et al. Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia. Invest Ophthalmol Vis Sci 2012 May [PMID: 22467573] (WB, Human) WB Human
Musset B, Clark RA, DeCoursey TE et al. NOX5 in human spermatozoa: expression, function, and regulation. J Biol Chem 2012 Mar [PMID: 22291013] (WB, Human) WB Human
Cao JY, Mansouri S, Frappier L. Changes in the Nasopharyngeal Carcinoma Nuclear Proteome Induced by the EBNA1 Protein of Epstein-Barr Virus Reveal Potential Roles for EBNA1 in Metastasis and Oxidative Stress Responses. J Virol. 2011 Oct 19. [PMID: 22013061]
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NOX1
Entrez
Uniprot