NOX1 Antibody

Images

 
Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by ...read more
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Human lung carcinoma. NOX1 antibody diluted at 1:500.
Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG ...read more
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] - Nox1 antibody detects Nox1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOX1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
Localization
Membrane, Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Application Notes
IP usage reported in scientific literature (PMID: 27094494). NOX1 antibody validated for FLOW from a verified customer review.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31546 in the following applications:

Publications
Read Publications using
NBP1-31546 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NOX1 Antibody

  • EC 1.6.3
  • GP91-2
  • Mitogenic oxidase 1
  • MOX-1
  • MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
  • NADPH oxidase 1
  • NADPH oxidase homolog-1
  • NOH1
  • NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
  • NOH-1NADPH oxidase 1 variant NOH-1L
  • NOX1
  • NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOX1 Antibody (NBP1-31546)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC-P, IHC-P WB, IP, WB.


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ICC/IF
(1)
IHC-P
(1)
IHC-P WB
(1)
IP
(2)
WB
(12)
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Human
(10)
Mouse
(7)
Rat
(3)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-31546 Applications Species
Kumar Raut P, Park P Globular adiponectin antagonizes leptin-induced growth of cancer cells by modulating inflammasomes activation: Critical role of HO-1 signaling Biochemical Pharmacology Jul 1 2020 [PMID: 32745467]
Wang X, Zhang S, Ding Y et al. p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis Redox Biol May 11 2020 [PMID: 32422541] (WB, Mouse) WB Mouse
Wang C, Zhu L, Yuan W et al. Diabetes aggravates myocardial ischaemia reperfusion injury via activating Nox2-related programmed cell death in an AMPK-dependent manner J. Cell. Mol. Med. Apr 29 2020 [PMID: 32351005] (WB, Rat) WB Rat
Cao L, Li X, Xu R et al. DUOX2, a common modulator in preventive effects of monoamine-based antidepressants on water immersion restraint stress- and indomethacin- induced gastric mucosal damage European Journal of Pharmacology Mar 1 2020 [PMID: 32131022] (WB, Rat) WB Rat
Shin SK, Kim KO, Kim SH et al. Exogenous 8-hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1-NADPH oxidase signaling J. Gastroenterol. Hepatol. Jan 6 2020 [PMID: 31907970] (IHC-P WB, Human, Rat) IHC-P WB Human, Rat
Yoshimura Y Resveratrol slows cell growth by targeting the Warburg effect and stimulating mitochondria metabolism Thesis 2019 Jun 28 [PMID: 31304204] (WB, Human) WB Human
Diebold BA, Wilder SG, De Deken X et al. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR Methods Mol. Biol. 2019 Jun 08 [PMID: 31172474]
Kaushik DK, Bhattacharya A, Mirzaei R et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance J. Clin. Invest. May 13 2019 [PMID: 31081803] (WB, Human) WB Human
Vara D, Cifuentes-Pagano E, Pagano PJ, Pula G. A novel combinatorial technique for simultaneous quantification of oxygen radicals and aggregation reveals unexpected redox patterns in the activation of platelets by different physiopathological stimuli Haematologica 2019 Jan 24 [PMID: 30679320] (IP, Human, Mouse) IP Human, Mouse
Raut PK, Kim SH, Choi DY et al. Growth of breast cancer cells by leptin is mediated via activation of the inflammasome: Critical roles of estrogen receptor signaling and reactive oxygen species production. Biochem. Pharmacol. Jan 10 2019 [PMID: 30633869] (WB, Human) WB Human
Show All 22 Publications.

Review for NOX1 Antibody (NBP1-31546) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31546:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry NOX1 NBP1-31546
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reviewed by:
Renato Gaspar
Flow Human 01/24/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedPlatelets
SpeciesHuman
Lot42214

Comments

CommentsPlatelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOX1 Antibody (NBP1-31546) (0)

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Secondary Antibodies

 

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Recent Reviews

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Renato Gaspar
01/24/2019
Application: Flow
Species: Human

Bioinformatics

Gene Symbol NOX1
Entrez
Uniprot