CYBB/NOX2 Antibody (NL7) [PE]

Images

 
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [PE] [NBP1-41012PE] - analysis of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

CYBB/NOX2 Antibody (NL7) [PE] Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Reviewed Applications
Read 1 Review rated 5
using
NBP1-41012PE in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CYBB/NOX2 Antibody (NL7) [PE]

  • CGD
  • CGD91-phox
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.6.3
  • GP91-1
  • GP91PHOX
  • GP91-PHOX
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYBB/NOX2 Antibody (NBP1-41012PE) (0)

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Review for CYBB/NOX2 Antibody (NBP1-41012PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-41012PE:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CYBB/NOX2 NBP1-41012PE
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reviewed by:
Corey.
Flow Human 10/08/2013
View

Summary

ApplicationFlow Cytometry
Sample TestedPRIMARY HUMAN AML SPECIMEN FROM PERIPHERAL BLOOD
SpeciesHuman
Lot0309
CommentsI HIGHLY APPROVE OF THE ANTIBODY. MY STAINING OBSERVATIONS WERE POSITIVE. CONTROL: UNSTAINED CONTROL WITH DAPI

Primary Anitbody

Dilution RatioFACS BUFFER; 15 MINUTES AT 4 DEGREES, 30 MIN ON ICE BOTH IN THE DARK; DILUTIONS TESTED: 1:100,1:200,1:300,1:400,1:500

Details

Detection NotesBECKMAN COULTER GALLIOS
Wash DescriptionFACS BUFFER I.E. PBS+0.5% FETAL BOVINE SERUM, 5 MIN AT 1X REPETITION

Comments

CommentsI HIGHLY APPROVE OF THE ANTIBODY. MY STAINING OBSERVATIONS WERE POSITIVE. CONTROL: UNSTAINED CONTROL WITH DAPI

Product General Protocols

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FAQs for CYBB/NOX2 Antibody (NBP1-41012PE) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-41012APC
DyLight 350 Labeled NBP1-41012UV
DyLight 405 Labeled NBP1-41012V
DyLight 488 Labeled NBP1-41012G
DyLight 680 Labeled NBP1-41012FR
DyLight 755 Labeled NBP1-41012IR
PerCP Labeled NBP1-41012PCP

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Bioinformatics Tool for CYBB/NOX2 Antibody (NBP1-41012PE)

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Recent Reviews

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Corey.
10/08/2013
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CYBB