Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected ...read more
Immunohistochemistry-Paraffin: NCF1 Antibody [NB100-790] - Staining of paraffin embedded Human Colon. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ...read more
Western Blot: NCF1 Antibody [NB100-790] - Staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), ...read more
WB: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). Use in immunoprecipitation reported in scientific literature (PMID:28725637).
NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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