NCF1 Antibody

Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NCF1 Antibody [NB100-790] - Staining of paraffin embedded Human Colon. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: NCF1 Antibody [NB100-790] - Staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Po, Ba, Rt, Bv
• Applications: WB, ELISA, Flow, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NCF1 Antibody Summary

• Immunogen: Peptide with sequence C-SESTKRKLASAV corresponding to C-Terminus according to NP_000256.3.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:20000
  • Western Blot 0.01 - 1 ug/mL
• Application Notes: WB: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). Use in immunoprecipitation reported in scientific literature (PMID:28725637).
• Control:

  NCF1 Overexpression Lysate

Reactivity Notes

Bacteria reactivity reported in scientific literature (PMID: 28725637).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCF1 Antibody

• 47 kDa neutrophil oxidase factor
• FLJ79451
• NADPH oxidase organizer 2
• NCF-1
• NCF1A
• neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein
• neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1)
• neutrophil cytosolic factor 1
• neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
• Neutrophil NADPH oxidase factor 1
• Nox organizer 2
• NOXO2NCF-47K
• Nox-organizing protein 2
• p47phox
• SH3 and PX domain-containing protein 1A
• SH3PXD1Ap47-phox

Background

NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCF1 Antibody (NB100-790)(4)

Publications using NB100-790

• Johnson JL, Ramadass M, Rahman F et al. The atypical small GTPase GEM/Kir is a negative regulator of the NADPH oxidase and NETs production through macroautophagy Journal of leukocyte biology 2021-06-04 [PMID: 34085299] (IF/IHC)
• Zhou L, Tang J et al. Five Constituents in Psoralea corylifolia L. Attenuate Palmitic Acid-Induced Hepatocyte Injury via Inhibiting the Protein Kinase C-alpha/Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Pathway. Front Pharmacol 2020-03-03 [PMID: 32116659] (ICC/IF, Mouse)
• Roberts JoAnn S, Atanasova Kalina R, Lee Jungnam et al. Opportunistic Pathogen Porphyromonas gingivalis Modulates Danger Signal ATP-Mediated Antibacterial NOX2 Pathways in Primary Epithelial Cells. Frontiers in Cellular and Infection Microbiology 2017-07-04 [PMID: 28725637] (IP, Bacteria)
• Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002-04-01 [PMID: 11903052]

Bioinformatics

• Gene Symbol: NCF1
• Entrez:
  • 653361(Human)
  • 17969(Mouse)
  • 114553(Rat)
• Uniprot:
  • NP_000256.3(Human)