Rac1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-91266] - Mouse Brain (cerebral cortex), Image is courtesy of customer review.
Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely ...read more
Immunocytochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord using Rabbit antibody to RAC1, RAC2 (100-150) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the ...read more
Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord (ventral horn) using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Rac1 Antibody Summary

Immunogen
A synthetic peptide from aa region 100-150 as a part of human RAC1, RAC2 (Ras-like protein TC25, p21-Rac2) conjugated to blue carrier protein was used as the antigen.
Specificity
Detects both RAC1 (Gene ID: 5879, UniProt: P63000) and RAC2 (Gene ID: 5880, UniProt: P15153).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAC1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:300-1:2000
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Immunocytochemistry
Control
Rac1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-91266 in the following applications:

Publications
Read Publications using
NB100-91266 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Predicted cross-reactivity based on antigen identity: Guinea Pig, Bovine, Xenopus, Dog, Zebrafish, Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Rac1 Antibody

  • Cell migration-inducing gene 5 protein
  • MGC111543
  • p21-Rac1
  • p21-Rac1migration-inducing gene 5
  • Rac1
  • Ras-like protein TC25
  • ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1)
  • ras-related C3 botulinum toxin substrate 1
  • rho family, small GTP binding protein Rac1
  • TC25
  • TC-25

Background

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. It is regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Rac 1: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Rac 2 is Hematopoietic specific. Defects in Rac 2 are the cause of neutrophil immunodeficiency syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF

Publications for Rac1 Antibody (NB100-91266)(3)

Review for Rac1 Antibody (NB100-91266) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-91266:
Filter by Applications
IHC
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry Rac1 NB100-91266
Enlarge
reviewed by:
Chansol Hurr
IHC Mouse 05/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain,Brain (cerebral cortex)
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Rac1 Antibody (NB100-91266) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Rac1 Antibody (NB100-91266)

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Blogs on Rac1.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

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Recent Reviews

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Chansol Hurr
05/11/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol RAC1
Entrez
Uniprot