Nox4 Antibody - BSA Free

Images

 
Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58849] - Porcine aortic endothelial cells stained with NB110-58849 (green). Nuclei were counterstained with DAPI (blue). Image from verified ...read more
Immunohistochemistry-Paraffin: Nox4 Antibody - BSA Free [NB110-58849] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of NOX4 antibody (NB110-58849). The staining was developed using HRP labeled ...read more
Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in HUVEC whole cell lysate. Lanes 1 and 2: serum-starved HUVEC lysate denatured at 95C for 5 minutes. Lanes 3: serum-starved HUVEC lysate ...read more
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in mouse brain showing positive staining in neurons in the cortex. Image provided by Rachel Reith.
Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in proximal convoluted tubules of the kidney using NB110-58849 at 5 ug/mL.
Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58849] - AT1-KO mice are resistant to alcohol-induced oxidative stress. Oxidative stress was examined by immunohistochemical staining of NOX4 (D) expression was ...read more
Biological Strategies: Knockdown Validated: Nox4 Antibody - BSA Free [NB110-58849] - DSC inhibits Nox4-mediated redox imbalance in BMDM. BMDM was treated with or without DSC (50 umol/L) or Nox4 siRNA. ...read more
Knockdown Validated: Nox4 Antibody - BSA Free [NB110-58849] - Nox4 Antibody [NB110-58849] - Silencing of NOX4 in H4-II-C3 rat hepatoma cell line. Image from verified customer review.
Presence of NOX4 in human ovarian tissue. Immunohistochemistry using human ovarian sections and an anti-NOX4 antibody from ProSci showed positive staining for NOX4 in granulosa (GC) and theca cells (TC) of a secondary ...read more
AT1-KO mice are resistant to alcohol-induced oxidative stress. Oxidative stress was examined by immunohistochemical staining of 3-NT (A) and 4-HNE (B). NOX2 (C) and NOX4 (D) expression was examined by ...read more
Blockade of LTCC suppressed CaMKII-NF-kB pathway in DOX-treated hearts. (c-g) Representative immunoblotsa and quantitative analysis of ERK, phosphorylated ERK, JNK, phosphorylated JNK, Nox4, p53, and GAPDH in each group ...read more
The representative images of immunohistochemical staining (A-D; sections were counterstained with hematoxylin; original magnification, x 20), and western blot assays (E,F) showed that FLL treatment decreased Nox4 ...read more
Fbs stimulated by BMM differentiate into myofibroblasts via the Cyr61/Nox4 pathway. (A) Western blotting for Fb differentiation, involving analysis of specific factors such as FSP-1, NOX2, NOX4, collagen-1, and Cyr61. ...read more
Fasting protects against oxidative stress resulting after two weeks of unilateral ischemia-reperfusion (IR) injury. (B) Immunoblots (left) and quantification (right) of catalase (CAT), glutamate-cysteine ligase ...read more
SPA0355 decreased renal oxidative stress and regulated levels of pro-oxidant and antioxidant enzymes in LPS-treated mice. (D) Representative images of Western blotting of nicotinamide adenine dinucleotide phosphate ...read more
Diabetic atrophied muscles exhibited a state of heightened oxidative stress (HSOS). (e and f) Muscle NADPH oxidase-related isoforms including NOX2 and NOX4 were determined at the mRNA (e) and protein levels (f) using ...read more
cCK-18 is formed following activation of the UPR. A. Immunoblots for phospho-IRE1 (p-IRE1), total IRE1 (IRE1), and cleaved cytokeratin 18 (cCK-18) showing time-dependent increase of cCK-18 in A549 cells exposed to 0.5 ...read more
Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% and 30%. Total RNA was extracted and RT-qPCR was performed in control (Ctrl) and stretched (Stretch) cells for myelin genes Mag (a, ...read more
NLRP3 inhibition with MCC950 reversed myocardial fibrosis induced by glucose fluctuations. (A) Representative images of hematoxylin and eosin (HE) staining for myocardial tissue in the FBG + NaCl and FBG + MCC950 groups ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, WB, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Nox4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].
Localization
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 28152080)
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockdown Validated
  • Western Blot 2 ug/ml
Application Notes
In Western blot this antibody recognizes a band at ~67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band ~48 kDa. The observed band size may vary depending on sample type and glycosylation. In ICC/IF cytoplasmic and mitochondrial staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control Peptide
Nox4 Peptide (NB110-58849PEP)
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB110-58849 in the following applications:

Publications
Read Publications using
NB110-58849 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32853822). Porcine reactivity reported in scientific literature (PMID: 24403605).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nox4 Antibody - BSA Free

  • EC 1.6.3
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX
  • KOX-1
  • KOX-1Renal NAD(P)H-oxidase
  • NADPH oxidase 4
  • Nox4
  • RENOX
  • RENOXEC 1.6.3.-

Background

NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which complex with CYBA/p22phox for generating superoxide intracellularly and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatases inhibition and function as oxygen sensor regulating the KCNK3/TASK-1 potassium channel as well as HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF- beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather thanO2 and this difference in the species generated underlie NOX-specific actions in cell signaling. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, and cardiovascular and renal complications of diabetes and in remodeling of pulmonary arteries in hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nox4 Antibody (NB110-58849)(108)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Rabbit.

We have publications tested in 10 applications: B/N, ELISA, IB, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
B/N
(1)
ELISA
(1)
IB
(1)
ICC/IF
(7)
IF
(2)
IF/IHC
(6)
IHC
(2)
IHC-Fr
(2)
IHC-P
(6)
WB
(71)
All Applications
Filter By Species
Human
(33)
Mouse
(39)
Rat
(15)
Bovine
(1)
Porcine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using NB110-58849 Applications Species
Bonanni R, Abbondante L, Cariati I et al. Metallosis after Hip Arthroplasty Damages Skeletal Muscle: A Case Report Geriatrics (Basel) 2023-09-15 [PMID: 37736892] (IHC) IHC
Milara J, MartInez-ExpOsito F, Montero P et al. N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro International Journal of Molecular Sciences 2022-11-22 [PMID: 36498845] (IHC-P, Human)

Details:
differentiated 937 macrophages were stimulated
IHC-P Human
Jiang J, Huang K, Xu S et al. Targeting NOX4 alleviates sepsis-induced acute lung injury via attenuation of redox-sensitive activation of CaMKII/ERK1/2/MLCK and endothelial cell barrier dysfunction Redox Biol
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 2023-07-01 [PMID: 37457144]
Pandey J, Larson-Casey JL, Patil MH et al. NOX4-TIM23 interaction regulates NOX4 mitochondrial import and metabolic reprogramming The Journal of biological chemistry 2023-04-10 [PMID: 37044213] (WB, Human, Mouse) WB Human, Mouse
Jo J, Kim JY, Leem J Protective Effects of Orexin A in a Murine Model of Cisplatin-Induced Acute Kidney Injury Journal of clinical medicine 2022-12-03 [PMID: 36498769] (WB, Mouse) WB Mouse
Jiang Z, Wu L, van der Leeden B et al. NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients International Journal of Cardiology 2022-11-01 [PMID: 36332749] (IHC-P, Human)

Details:
Diltuion used for IHC 1:100
IHC-P Human
Piao SG, Ding J, Lin XJ et al. Inhibition of RIP1-RIP3-mediated necroptosis attenuates renal fibrosis via Wnt3alpha/beta-catenin/GSK-3beta signaling in unilateral ureteral obstruction PloS one 2022-10-12 [PMID: 36223414]
Chen YY, Wu TT, Ho CY et al. Blocking of SGLT2 to Eliminate NADPH-Induced Oxidative Stress in Lenses of Animals with Fructose-Induced Diabetes Mellitus International journal of molecular sciences 2022-06-27 [PMID: 35806147]
Show All 108 Publications.

Reviews for Nox4 Antibody (NB110-58849) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB110-58849:
Filter by Applications
WB
(6)
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Nox4 NB110-58849
Enlarge
5
reviewed by:
Verified Customer
WB Rat 03/01/2018
View

Summary

ApplicationWestern Blot
Sample TestedH4-II-E-C3 rat hepatoma cell line
SpeciesRat
Lotn
Immunofluorescence Nox4 NB110-58849
Enlarge
4
reviewed by:
Verified Customer
IF Other 07/25/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedporcine aortic endothelial cells
SpeciesOther
LotL
Western Blot Nox4 NB110-58849
Enlarge
4
reviewed by:
Verified Customer
WB Other 07/22/2016
View

Summary

ApplicationWestern Blot
Sample TestedPAOEC cell lysate
SpeciesOther
LotL
  5
reviewed by:
Paramananda Saikia
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
LotD
  5
reviewed by:
Verified Customer
WB Other 06/13/2014
View

Summary

ApplicationWestern Blot
Sample TestedSH-SY5Y whole cell lyste
SpeciesOther
LotD
Immunohistochemistry-Paraffin Nox4 NB110-58849
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 01/30/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse
Western Blot Nox4 NB110-58849
Enlarge
4
reviewed by:
Vivian Lin
WB Human 12/10/2012
View

Summary

ApplicationWestern Blot
Sample TestedHUVEC whole cell lysate
SpeciesHuman
LotE
Western Blot Nox4 NB110-58849
Enlarge
5
reviewed by:
Verified Customer
WB Human 11/15/2012
View

Summary

ApplicationWestern Blot
Sample TestedIMR90 fibroblast, +/- TGF beta, whole cell lysate
SpeciesHuman
LotE4, E6, E7

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nox4 Antibody (NB110-58849). (Showing 1 - 3 of 3 FAQs).

  1. Do you have an alternative product for NOX4 Antibody (NB110-58851). I will be using it for porcine.
    • NB110-58849 is validated in the same species (apart from porcine) and applications as NB110-58851 and should be a suitable alternative for you. NB110-58851 was raised to a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein, whilst NB110-58849 was raised to a synthetic peptide made to an internal region (between residues 100-200) of the human NOX4 protein. Both antibodies are rabbit polyclonals. As NB110-58849 has not yet been tested in porcine samples, you may be interested in our Innovator's Reward programme. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply need to go to the antibody’s webpage and complete an online review with an image, detailing the positive or negative results of your study. In return you will receive a discount voucher for 100% of the purchase price of the reviewed product. More details of this programme can be found here: Innovator's Reward Program. I ran an alignment for you between the human NOX4 sequence and an unreviewed porcine NOX4 sequence from Uniprot (accession number F1STQ7.). The homology is high between the two proteins. Please do, however, be aware that this porcine sequence is unreviewed.
  2. Is NB110-58849PEP the exact peptide sequence you raised your polyclonal antibody against? And, do you have any Westerns or IHC images with your NOX4 antibody combined with this as a blocking peptide and showing very little background?
    • We have used this blocking peptide in our testing in the past; however, I am unable to find any available images on our system. NOX4 does tend to be difficult to work with in WB, as there are 9 different isoforms of the protein. However, we will guarantee that the blocking peptide and the antibody will perform together.
  3. What is the molecular weight of NOX4 (for western blot)?
    • The MW of this protein is Length:578 Mass (Da):66,932 on Uniprot. There are Application Notes for this antibody: In Western blot this antibody recognizes a band at ~67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band ~48 kDa. The observed band size may vary depending on sample type and glycosylation.

Secondary Antibodies

 

Isotype Controls

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Blogs on Nox4.

NOX4 Antibodies: Don't NOX them until you've tried them
NOX4 is an NADPH oxidase that generates superoxide within the cell. It is primarily found in vascular cells, fibroblasts, and osteoclasts, with abundant expression in the kidney. Unlike its family members NOX1 and NOX2, NOX4 is constitutively active, ...  Read full blog post.

NOX4 Antibodies in Diabetic Nephropathy Research
Diabetic nephropathy (DN) is one of the leading complications resulting from chronic diabetes. It manifests as progressive renal failure caused by mesangial cell hyperplasia and fibrosis, and is one of the leading causes of terminal kidney disease (1)...  Read full blog post.

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Recent Reviews

4.5
5
4
4
4
3
0
2
0
1
0

Verified Customer
03/01/2018
Application: WB
Species: Rat

Verified Customer
07/25/2016
Application: IF
Species: Other

Verified Customer
07/22/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol NOX4