Nox4 Antibody - BSA Free

Images

 
Immunohistochemistry: Nox4 Antibody [NB110-58849] - AT1-KO mice are resistant to alcohol-induced oxidative stress. Oxidative stress was examined by immunohistochemical staining of NOX4 (D) expression was examined by ...read more
Biological Strategies: Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.
Biological Strategies: Knockdown Validated: Nox4 Antibody [NB110-58849] - Silencing of NOX4 in H4-II-C3 rat hepatoma cell line. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody [NB110-58849] - Porcine aortic endothelial cells stained with NB110-58849 (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.
Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 in HUVEC whole cell lysate. Lanes 1 and 2: serum-starved HUVEC lysate denatured at 95C for 5 minutes. Lanes 3: serum-starved HUVEC lysate denatured at room ...read more
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody [NB110-58849] - Detection of NOX4 in mouse brain showing positive staining in neurons in the cortex. Image provided by Rachel Reith.
Immunohistochemistry: Nox4 Antibody [NB110-58849] - Detection of NOX4 in proximal convoluted tubules of the kidney using NB110-58849 at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, Rb, ShSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Nox4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].
Localization
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockdown Validated
  • Western Blot 2 ug/ml
Application Notes
Immunohistochemistry-Frozen was reported in scientific literature. In Western blot this antibody recognizes a band at ~67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band ~48 kDa. The observed band size may vary depending on sample type and glycosylation. In ICC/IF cytoplasmic and mitochondrial staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunoblotting reported in scientific literature (PMID 28152080).
Control Peptide
Nox4 Peptide (NB110-58849PEP)
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB110-58849 in the following applications:

Publications
Read Publications using
NB110-58849 in the following applications:

  • IB
    1 publication
  • 7 publications
  • IF
    2 publications
  • IHC
    6 publications
  • 2 publications
  • 4 publications
  • KD
    1 publication
  • WB
    67 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32853822). Porcine reactivity reported in scientific literature (PMID: 24403605).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nox4 Antibody - BSA Free

  • EC 1.6.3
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX
  • KOX-1
  • KOX-1Renal NAD(P)H-oxidase
  • NADPH oxidase 4
  • Nox4
  • RENOX
  • RENOXEC 1.6.3.-

Background

NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which complex with CYBA/p22phox for generating superoxide intracellularly and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatases inhibition and function as oxygen sensor regulating the KCNK3/TASK-1 potassium channel as well as HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF- beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather thanO2 and this difference in the species generated underlie NOX-specific actions in cell signaling. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, and cardiovascular and renal complications of diabetes and in remodeling of pulmonary arteries in hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nox4 Antibody (NB110-58849)(95)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Rabbit.

We have publications tested in 8 applications: IB, ICC/IF, IF, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
IB
(1)
ICC/IF
(7)
IF
(2)
IHC
(6)
IHC-Fr
(2)
IHC-P
(4)
KD
(1)
WB
(67)
All Applications
Filter By Species
Human
(30)
Mouse
(38)
Rat
(15)
Bovine
(1)
Porcine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 95. Show All 95 Publications.
Publications using NB110-58849 Applications Species
Baylan U, Korn A, Emmens RW et al. Liraglutide treatment attenuates inflammation markers in the cardiac, cerebral and renal microvasculature in streptozotocin-induced diabetic rats European journal of clinical investigation [PMID: 35488737] (IHC-P, Rat) IHC-P Rat
Fukatsu M, Ohkawara H, Wang X et al. The suppressive effects of Mer inhibition on inflammatory responses in the pathogenesis of LPS-induced ALI/ARDS Science signaling Mar 8 2022 [PMID: 35258998]
Xuan MY, Piao SG, Ding J et al. Dapagliflozin Alleviates Renal Fibrosis by Inhibiting RIP1-RIP3-MLKL-Mediated Necroinflammation in Unilateral Ureteral Obstruction Frontiers in pharmacology Jan 7 2022 [PMID: 35069210] (WB, Rat) WB Rat
Ding J, Jin J, Na Lei Y et al. Exogenous pancreatic kininogenase protects against tacrolimus-induced renal injury by inhibiting PI3K/AKT signaling: The role of bradykinin receptors International immunopharmacology Jan 20 2022 [PMID: 35066448] (WB, Rat) WB Rat
Youn J, Zhang Y, Wu Y Et al. Therapeutic application of estrogen for COVID-19: Attenuation of IL-6 and SARS-CoV-2 spike protein stimulated NOX2 activation and ROS production in endothelial cells Redox Biology Aug 1 2021 [PMID: 34509916] (WB, Human) WB Human
Chiou JT, Shi YJ, Lee YC et al. Carboxyl group-modified alpha-lactalbumin induces TNF-alpha-mediated apoptosis in leukemia and breast cancer cells through the NOX4/p38 MAPK/PP2A axis International journal of biological macromolecules Jul 24 2021 [PMID: 34310992] (WB) WB
Li X, Chen X, Liu Y et al. The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase Front. Genet. Oct 2 2020 [PMID: 33133132] (WB, Human, Mouse) WB Human, Mouse
Jiang J, Huang K, Xu S et al. Targeting NOX4 alleviates sepsis-induced acute lung injury via attenuation of redox-sensitive activation of CaMKII/ERK1/2/MLCK and endothelial cell barrier dysfunction Redox Biology Sep 1 2020 (WB, KD, Mouse) WB, KD Mouse
Larson-Casey JL, Gu L, Kang J et al. NOX4 regulates macrophage apoptosis resistance to induce fibrotic progression The Journal of biological chemistry May 20 2021 [PMID: 34023385] (WB, Human, Mouse) WB Human, Mouse
Chiou JT, Huang NC, Huang CH et al. NOXA-mediated degradation of MCL1 and BCL2L1 causes apoptosis of daunorubicin-treated human acute myeloid leukemia cells Journal of cellular physiology May 13 2021 [PMID: 33982799]
Show All 95 Publications.

Reviews for Nox4 Antibody (NB110-58849) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB110-58849:
Filter by Applications
Western Blot
(6)
Immunofluorescence
(1)
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Nox4 NB110-58849
Enlarge
5
reviewed by:
Wenliang Zhang
Western Blot Rat 03/01/2018
View

Summary

ApplicationWestern Blot
Sample TestedH4-II-E-C3 rat hepatoma cell line
SpeciesRat
Lotn
Immunofluorescence Nox4 NB110-58849
Enlarge
4
reviewed by:
silvia agostini
Immunofluorescence Other 07/25/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedporcine aortic endothelial cells
SpeciesOther
LotL
Western Blot Nox4 NB110-58849
Enlarge
4
reviewed by:
silvia agostini
Western Blot Other 07/22/2016
View

Summary

ApplicationWestern Blot
Sample TestedPAOEC cell lysate
SpeciesOther
LotL
  5
reviewed by:
Verified Customer
Western Blot Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
LotD
  5
reviewed by:
Ning Wang
Western Blot Other 06/13/2014
View

Summary

ApplicationWestern Blot
Sample TestedSH-SY5Y whole cell lyste
SpeciesOther
LotD
Immunohistochemistry-Paraffin Nox4 NB110-58849
Enlarge
4
reviewed by:
Rachel Reith
Immunohistochemistry-Paraffin Mouse 01/30/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse
Western Blot Nox4 NB110-58849
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 12/10/2012
View

Summary

ApplicationWestern Blot
Sample TestedHUVEC whole cell lysate
SpeciesHuman
LotE
Western Blot Nox4 NB110-58849
Enlarge
5
reviewed by:
Naomi Logsdon
Western Blot Human 11/15/2012
View

Summary

ApplicationWestern Blot
Sample TestedIMR90 fibroblast, +/- TGF beta, whole cell lysate
SpeciesHuman
LotE4, E6, E7

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nox4 Antibody (NB110-58849). (Showing 1 - 3 of 3 FAQs).

  1. Do you have an alternative product for NOX4 Antibody (NB110-58851). I will be using it for porcine.
    • NB110-58849 is validated in the same species (apart from porcine) and applications as NB110-58851 and should be a suitable alternative for you. NB110-58851 was raised to a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein, whilst NB110-58849 was raised to a synthetic peptide made to an internal region (between residues 100-200) of the human NOX4 protein. Both antibodies are rabbit polyclonals. As NB110-58849 has not yet been tested in porcine samples, you may be interested in our Innovator's Reward programme. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply need to go to the antibody’s webpage and complete an online review with an image, detailing the positive or negative results of your study. In return you will receive a discount voucher for 100% of the purchase price of the reviewed product. More details of this programme can be found here: Innovator's Reward Program. I ran an alignment for you between the human NOX4 sequence and an unreviewed porcine NOX4 sequence from Uniprot (accession number F1STQ7.). The homology is high between the two proteins. Please do, however, be aware that this porcine sequence is unreviewed.
  2. Is NB110-58849PEP the exact peptide sequence you raised your polyclonal antibody against? And, do you have any Westerns or IHC images with your NOX4 antibody combined with this as a blocking peptide and showing very little background?
    • We have used this blocking peptide in our testing in the past; however, I am unable to find any available images on our system. NOX4 does tend to be difficult to work with in WB, as there are 9 different isoforms of the protein. However, we will guarantee that the blocking peptide and the antibody will perform together.
  3. What is the molecular weight of NOX4 (for western blot)?
    • The MW of this protein is Length:578 Mass (Da):66,932 on Uniprot. There are Application Notes for this antibody: In Western blot this antibody recognizes a band at ~67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band ~48 kDa. The observed band size may vary depending on sample type and glycosylation.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nox4 Antibody (NB110-58849)

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Blogs on Nox4.

NOX4 Antibodies: Don't NOX them until you've tried them
NOX4 is an NADPH oxidase that generates superoxide within the cell. It is primarily found in vascular cells, fibroblasts, and osteoclasts, with abundant expression in the kidney. Unlike its family members NOX1 and NOX2, NOX4 is constitutively active, ...  Read full blog post.

NOX4 Antibodies in Diabetic Nephropathy Research
Diabetic nephropathy (DN) is one of the leading complications resulting from chronic diabetes. It manifests as progressive renal failure caused by mesangial cell hyperplasia and fibrosis, and is one of the leading causes of terminal kidney disease (1)...  Read full blog post.

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Recent Reviews

4.5
5
4
4
4
3
0
2
0
1
0

Wenliang Zhang
03/01/2018
Application: Western Blot
Species: Rat

silvia agostini
07/25/2016
Application: Immunofluorescence
Species: Other

silvia agostini
07/22/2016
Application: Western Blot
Species: Other

Bioinformatics

Gene Symbol NOX4