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AKAP13 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: AKAP13 Antibody [NBP1-89163] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human heart shows moderate to strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AKAP13 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ARELKEQLHQKDQKILLLLEEKEMIFRDMAECSTPLPEDCSPTHSPRVLFRSNTEEALKGGPLMKSAINEVEILQGLVSGNLGGTLGPTVSSPIEQDVVGPVSLPR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
AKAP13 Protein (NBP1-89163PEP)
Publications
Read Publication using
NBP1-89163 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AKAP13 Antibody

  • A kinase (PRKA) anchor protein 13
  • AKAP-LbcAKAP-13
  • A-kinase anchor protein 13
  • A-kinase anchoring protein
  • ARHGEF13
  • Breast cancer nuclear receptor-binding auxiliary protein
  • BRXNon-oncogenic Rho GTPase-specific GTP exchange factor
  • c-lbc
  • FLJ11952
  • FLJ43341
  • Guanine nucleotide exchange factor Lbc
  • HA-3
  • HT31
  • Human thyroid-anchoring protein 31
  • LBCLBC oncogene
  • Lymphoid blast crisis oncogeneProtein kinase A-anchoring protein 13
  • p47
  • PROTO-LB
  • PROTO-LBC

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in at least 3 transcript variants encoding different isoforms containing a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and, in addition, function as protein kinase A-anchoring proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP13 Antibody (NBP1-89163)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKAP13