Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: 1009 (1 ug/mL), 1012 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NBP1-77067] - HeLa cells with CXCR4 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-77067] - Human spleen tissue with CXCR4 antibody at 5 ug/mL.
Genetic Strategies: Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and ...read more
Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.
Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: ...read more
Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 ...read more
Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.
Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: 1009 (2 ug/mL) or 1012 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRPconjugate ...read more
Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: 1009 (2 ug/mL), 1 hincubation ...read more
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NBP1-77067] - HeLa cells with CXCR4 antibody at 20 ug/mL.
Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.
Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4. The immunogen is located within the first 50 amino acids of CXCR4.
Specificity
CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXCR4
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CXCR4 Antibody (NBP1-77067). (Showing 1 - 1 of 1 FAQs).
Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.
Bioinformatics Tool for CXCR4 Antibody (NBP1-77067)
Discover related pathways, diseases and genes to CXCR4 Antibody (NBP1-77067). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for CXCR4 Antibody (NBP1-77067)
Discover more about diseases related to CXCR4 Antibody (NBP1-77067).
LAMP2: Protector of the lysosome LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m... Read full blog post.
CXCR4 Studies on Neural and Stem Cells The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li... Read full blog post.
Understanding CXCR4 and SDF1 CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our CXCR4 Antibody and receive a gift card or discount.