Collagen III alpha 1/COL3A1 Antibody [Biotin] (NB120-6580)

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Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [Biotin] [NB120-6580] - Strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Collagen III alpha 1/COL3A1 Antibody [Biotin] Summary

Immunogen
Collagen Type III from human and bovine placenta.
Localization
Extracellular matrix
Specificity
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1:100
Readout System
Publications
Read Publication using NB120-6580.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21479341). Rat reactivity reported in scientific literature (PMID: 23370982).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for Collagen III alpha 1/COL3A1 Antibody [Biotin]

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NB120-6580)(1)

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Bioinformatics

Gene Symbol COL3A1
Entrez