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Elastin Antibody (BA-4)

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 (2 Reviews)
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Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.
Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: ...read more
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin and GAGs following decellularization. Porcine vena cavas were decellularized and ...read more
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original ...read more
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.

Product Details

Summary
Reactivity Hu, Po, Ca, Fe, Gp, Gt, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
BA-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Elastin Antibody (BA-4) Summary

Immunogen
Bovine alpha-Elastin.
Localization
Connective tissue.
Specificity
This recognizes insoluble elastin, alpha-elastin, soluble non-cross linked precursor of elastin (tropoelastin).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELN
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30214669).. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:33091431).
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB100-2076 in the following applications:
Publications
Read Publications using
NB100-2076 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 30214669). Feline reactivity reported in scientific literature (PMID:33091431

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Ascites
Preservative
0.09% Sodium Azide
Purity
Ascites

Alternate Names for Elastin Antibody (BA-4)

  • elastin
  • FLJ38671
  • FLJ43523
  • SVAS
  • tropoelastin
  • WBS
  • WS

Background

Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - Expression of Lox and Loxl1-Loxl4 mRNAs in orthotopic rat prostate tumours and in the surrounding normal rat prostate tissue. Representative sections from a 10-day orthotopic AT-1 rat prostate tumour (T), tumour-adjacent normal rat prostate tissue (N), and tumour-free control rat prostate tissue stained for LOX (inserts show LOX staining in higher magnifications). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep19608), licensed under a CC-BY licence.
Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
(11 Reviews)
(32 Publications)
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left).
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Western Blot: Fibulin 5/DANCE Antibody [NBP2-76929] - Analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
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Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: THP-1
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Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
(14 Publications)
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Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
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Western Blot: Cathepsin G Antibody [NBP2-92713] - Analysis of extracts of various cell lines, using Cathepsin G at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-92713] - Paraffin-embedded mouse spleen using Cathepsin G.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-92713] - Paraffin-embedded human appendix using Cathepsin G.
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Western Blot: AAAS Antibody (5A1) [H00008086-M02] - AAAS monoclonal antibody (M02), clone 5A1. Analysis of AAAS expression in NIH/3T3.
Immunohistochemistry-Paraffin: AAAS Antibody (5A1) [H00008086-M02] - Analysis of monoclonal antibody to AAAS on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
Western Blot: AAAS Antibody (5A1) [H00008086-M02] - Western Blot analysis of AAAS expression in HeLa ( Cat # L013V1 ).
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Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.
(5 Publications)
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Applications: WB, IHC, IHC-P

Publications for Elastin Antibody (NB100-2076)(5)

We have publications tested in 4 confirmed species: Human, Feline, Porcine, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


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(1)
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(1)
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(1)
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(1)
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Reviews for Elastin Antibody (NB100-2076) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NB100-2076:
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IHC-Fr
(1)
IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Elastin NB100-2076
Enlarge 		3
reviewed by:
Anonymous
 IHC-Fr Mouse 11/04/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult small intestine
SpeciesMouse
LotH379
Immunohistochemistry-Paraffin Elastin NB100-2076
Enlarge 		4
reviewed by:
Anonymous
 IHC-P Other 07/07/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPig testis FFPE
SpeciesOther
CommentsAlso worked well with fluorescent secondary. Required overnight incubation with primary.

Primary Anitbody

Dilution Ratio1:50

Details

Fixation DetailsFFPE HIER

Comments

CommentsAlso worked well with fluorescent secondary. Required overnight incubation with primary.

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WB Video Protocol
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Recent Reviews

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Immunohistochemistry-Frozen Elastin NB100-2076
Enlarge
 Anonymous
11/04/2017
Application: IHC-Fr
Species: Mouse
read this review
Immunohistochemistry-Paraffin Elastin NB100-2076
Enlarge
 Anonymous
07/07/2014
Application: IHC-P
Species: Other
read this review

Bioinformatics

Gene Symbol ELN
Entrez
Uniprot