Elastin Antibody (BA-4)

Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human renal cell carcinoma stained with Elastin antibody (NB100-2076)

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original magnification X100.

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin and GAGs following decellularization. Porcine vena cavas were decellularized and analyzed for ECM proteins. Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin and vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0220743), licensed under a CC-BY license.

• Reactivity: Hu, Bv, Fe, Rb
• Applications: WB, ICC/IF, IHC
• Clone: BA-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Elastin Antibody (BA-4) Summary

• Immunogen: Bovine alpha-Elastin.
• Localization: Connective tissue.
• Specificity: This recognizes insoluble elastin, alpha-elastin, soluble non-cross linked precursor of elastin (tropoelastin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELN
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30214669).. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:33091431).

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 30214669). Feline reactivity reported in scientific literature (PMID:33091431

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for Elastin Antibody (BA-4)

• elastin
• FLJ38671
• FLJ43523
• SVAS
• tropoelastin
• WBS
• WS

Background

Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Elastin Antibody (NB100-2076)(11)

Publications using NB100-2076

• Krasinski Z, Biskupski P, Dzieciuchowicz L et al. The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction. Eur J Vasc Endovasc Surg 2010-08-01 [PMID: 20570188] (IF/IHC, Human)
• Liu J, Feng Z, Liu P et al. Transcriptome Analysis of Human Vascular Smooth Muscle Cells Cultured on a Polyglycolic Acid Mesh Scaffold Journal of Tissue Engineering and Regenerative Medicine 2023-06-22 (WB, Human)
• Manon J, Evrard R, Fiev� L et al. A New Osteogenic Membrane to Enhance Bone Healing: At the Crossroads between the Periosteum, the Induced Membrane, and the Diamond Concept Bioengineering (Basel) 2023-01-21 [PMID: 36829637] (ICC/IF, IHC)
• Manon J, Evrard R, Maistriaux L et al. Periosteum and fascia lata: Are they so different? Frontiers in Bioengineering and Biotechnology 2022-10-19 [PMID: 36338112] (ICC/IF, IHC)
• Gonzalez BA, Herrera A, Ponce C et al. Stem Cell-Secreted Allogeneic Elastin-Rich Matrix with Subsequent Decellularization for the Treatment of Critical Valve Diseases in the Young Bioengineering (Basel, Switzerland) 2022-10-20 [PMID: 36290556] (IHC-Fr, Human)
• Gong H, Chen L, He Y et al. Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin Frontiers in cellular and infection microbiology 2022-08-11 [PMID: 36034702] (IHC-P, Porcine)
• Xiao W, Chen W, Wang Y et al. Recombinant DT beta 4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization Science advances 2022-07-15 [PMID: 35857526] (ICC/IF, Rat)
• Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)
• Simsa R, Vila XM, Salzer E et al. Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels PLoS ONE 2019-08-05 [PMID: 31381614] (IHC-P, Porcine)
• Phinikaridou A, Lacerda S, Lavin B. Tropoelastin: a novel marker for plaque progression and instability Circulation: Cardiovascular Imaging 1905-07-10 [PMID: 30214669] (IHC-Fr, Rabbit)
• Wrenn DS, Griffin GL, Senior RM, Mecham RP. Characterization of biologically active domains on elastin: identification of a monoclonal antibody to a cell recognition site. Biochemistry. 1986-09-09 [PMID: 2429696]

Reviews for Elastin Antibody (NB100-2076) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Other.

reviewed by: Verified Customer IHC-Fr Mouse 11/04/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult small intestine
• Species: Mouse
• Lot: H379

reviewed by: Verified Customer IHC-P Other 07/07/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pig testis FFPE
• Species: Other

Bioinformatics

• Gene Symbol: ELN
• Entrez:
  • 2006(Human)
• Uniprot:
  • P15502(Human)