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Elastin Antibody (BA-4)

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 (2 Reviews)
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Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.
Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: ...read more
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original ...read more

Product Details

Summary
Reactivity Hu, Po, Ca, Fe, GP, Gt, Rb, ShSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
BA-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Elastin Antibody (BA-4) Summary

Immunogen
Bovine alpha-Elastin.
Localization
Connective tissue.
Specificity
This recognizes insoluble elastin, alpha-elastin, soluble non-cross linked precursor of elastin (tropoelastin).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELN
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30214669).
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB100-2076 in the following applications:
Publications
Read Publications using
NB100-2076 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 30214669).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Ascites
Preservative
0.09% Sodium Azide
Purity
Ascites

Alternate Names for Elastin Antibody (BA-4)

  • elastin
  • FLJ38671
  • FLJ43523
  • SVAS
  • tropoelastin
  • WBS
  • WS

Background

Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Frozen: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Adult eye (blue) and muscle (red) tissue. An image was captured using epifluorescent microscope detecting signals from Alexa fluor 568 conjugated secondary antibody. Used at 1:100 dilution. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).
(1 Review)
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Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.
(2 Publications)
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Elastin Antibody
(BA-4)
NB100-2076
Elastin Antibody (BA-4)
Species: Hu, Po, Ca, Fe, GP, Gt, Rb, Sh
Applications: WB, IHC, IHC-Fr, IHC-P

Publications for Elastin Antibody (NB100-2076)(4)

We have publications tested in 3 confirmed species: Human, Porcine, Rabbit.

We have publications tested in 3 applications: IHC, IHC-Fr, IHC-P.


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IHC
(1)
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(1)
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(1)
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Reviews for Elastin Antibody (NB100-2076) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NB100-2076:
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IHC-Fr
(1)
IHC-P
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Elastin NB100-2076
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reviewed by:
Anonymous
 IHC-Fr Mouse 11/04/2017
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult small intestine
SpeciesMouse
LotH379
Immunohistochemistry-Paraffin Elastin NB100-2076
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reviewed by:
Anonymous
 IHC-P Other 07/07/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPig testis FFPE
SpeciesOther
CommentsAlso worked well with fluorescent secondary. Required overnight incubation with primary.

Primary Anitbody

Dilution Ratio1:50

Details

Fixation DetailsFFPE HIER

Comments

CommentsAlso worked well with fluorescent secondary. Required overnight incubation with primary.

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Immunohistochemistry-Frozen Elastin NB100-2076
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 Anonymous
11/04/2017
Application: IHC-Fr
Species: Mouse
read this review
Immunohistochemistry-Paraffin Elastin NB100-2076
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 Anonymous
07/07/2014
Application: IHC-P
Species: Other
read this review

Bioinformatics

Gene Symbol ELN
Entrez
Uniprot
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